Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1980 Oct;12(4):541–545. doi: 10.1128/jcm.12.4.541-545.1980

Serogrouping single colonies of beta-hemolytic streptococci from primary throat culture plates with nitrous acid extraction and Phadebact streptococcal reagents.

M Slifkin, G Interval
PMCID: PMC273632  PMID: 7419707

Abstract

The serogrouping of isolated beta-hemolytic streptococcal colonies from throat cultures by a micronitrous acid extraction method employing Phadebact Streptotoccus Test reagents was compared with results obtained with the direct-plate Phadebact procedure and the autoclave and Streptomyces albus enzyme-lysozyme extraction methods. These data were compared with those from the Lancefield grouping obtained with a capillary precipitin test. The micronitrous acid extraction method was modified to yield an uncomplicated method that provides specific coagglutination responses from one beta-hemolytic streptococcal colony that may be collected from a primary blood agar plate either on the end of an applicator stick or from a sweep of an inoculating loop from mixed growth.

Full text

PDF
541

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carlson J. R., McCarthy L. R. Modified coagglutination procedure for the serological grouping of streptococci. J Clin Microbiol. 1979 Mar;9(3):329–332. doi: 10.1128/jcm.9.3.329-332.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Facklam R. R., Cooksey R. C., Wortham E. C. Evaluation of commercial latex agglutination reagents for grouping streptococci. J Clin Microbiol. 1979 Nov;10(5):641–646. doi: 10.1128/jcm.10.5.641-646.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Facklam R. R., Padula J. F., Thacker L. G., Wortham E. C., Sconyers B. J. Presumptive identification of group A, B, and D streptococci. Appl Microbiol. 1974 Jan;27(1):107–113. doi: 10.1128/am.27.1.107-113.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Finch R. G., Phillips I. Serological grouping of streptococci by a slide coagglutination method. J Clin Pathol. 1977 Feb;30(2):168–170. doi: 10.1136/jcp.30.2.168. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kholy A. E., Facklam R., Sabri G., Rotta J. Serological identification of group A streptococci from throat scrapings before culture. J Clin Microbiol. 1978 Dec;8(6):725–728. doi: 10.1128/jcm.8.6.725-728.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lue Y. A., Howit I. P., Ellner P. D. Rapid grouping of beta-hemolytic streptococci by latex agglutination. J Clin Microbiol. 1978 Sep;8(3):326–328. doi: 10.1128/jcm.8.3.326-328.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Matthieu D. E., Jr, Wasilauskas B. L., Stallings R. A. A rapid staphylococcal coagglutination technic to differentiate group A from other streptococcal groups. Am J Clin Pathol. 1979 Sep;72(3):463–467. doi: 10.1093/ajcp/72.3.463. [DOI] [PubMed] [Google Scholar]
  8. RANTZ L. A., RANDALL E. Use of autoclaved extracts of hemolytic streptococci for serological grouping. Stanford Med Bull. 1955 May;13(2):290–291. [PubMed] [Google Scholar]
  9. Slifkin M., Engwall C., Pouchet G. R. Direct-plate serological grouping of beta-hemolytic streptococci from primary isolation plates with the Phadebact streptococcus test. J Clin Microbiol. 1978 Apr;7(4):356–360. doi: 10.1128/jcm.7.4.356-360.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Slifkin M., Pouchet-Melvin G. R. Evaluation of three commercially available test products for serogrouping beta-hemolytic streptococci. J Clin Microbiol. 1980 Mar;11(3):249–255. doi: 10.1128/jcm.11.3.249-255.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Swanson J., Hsu K. C., Gotschlich E. C. Electron microscopic studies on streptococci. I. M antigen. J Exp Med. 1969 Nov 1;130(5):1063–1091. doi: 10.1084/jem.130.5.1063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Watson B. K., Moellering R. C., Jr, Kunz L. J. Identification of streptococci: use of lysozyme and Streptomyces albus filtrate in the preparation of extracts for Lancefield grouping. J Clin Microbiol. 1975 Mar;1(3):274–278. doi: 10.1128/jcm.1.3.274-278.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. el Kholy A., Wannamaker L. W., Krause R. M. Simplified extraction procedure for serological grouping of beta-hemolytic streptococci. Appl Microbiol. 1974 Nov;28(5):836–839. doi: 10.1128/am.28.5.836-839.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES