Table 1.
Amino Acid Composition of mfp- 1s from M. californianus, M. galloprovincialis (13), and M. edulis (19), respectively, in Mole % (res/100 res)a
| Mcfp-1 |
Mgfp-1 |
Mefp-1 |
||||
|---|---|---|---|---|---|---|
| amino acid | actual | virtual | actual | virtual | actual | virtual |
| di-Hyp | 5.9 | 4.1 | 3.0 | |||
| Hyp | 18.2 | 10.7 | 10.2 | |||
| Asx | 2.6 | 0.7 | 3.8 | 1.1 | 2.3 | 0.8 |
| Thr | 9.7 | 11.4 | 10.9 | 10.5 | 11.7 | 12.2 |
| Ser | 9.7 | 11.2 | 8.4 | 12.3 | 10.2 | 9.9 |
| Glx | 1.8 | 0.6 | 2.3 | 0.3 | 0.9 | 0.2 |
| Pro | 3.8 | 24.4 | 6.7 | 24.8 | 8.1 | 25.6 |
| Gly | 2.4 | 1.0 | 3.3 | 1.0 | 3.1 | 0.1 |
| Ala | 4.2 | 3.4 | 7.5 | 7.0 | 8.1 | 7.5 |
| Cys | 0 | 0 | 0.0 | 0.0 | 0.0 | 0.0 |
| Val | 1.3 | 0.7 | 1.7 | 0.5 | 0.8 | 0.5 |
| Met | 0 | 0.1 | 0 | 0.1 | 0.1 | 0.1 |
| Ile | 3.2 | 3.7 | 1.0 | 1.1 | 0.8 | 0.7 |
| Leu | 1.1 | 0.8 | 1.1 | 1.1 | 1.1 | 1.0 |
| Dopa | 13.0 | 10.6 | 10.5 | |||
| Tyr | 5.8 | 19.4 | 7.2 | 19.6 | 6.6 | 19.2 |
| Phe | 0.4 | 0.1 | 0.4 | 0.1 | 0.1 | 0.0 |
| His | 0.4 | 0.6 | 0.6 | 0.4 | 0.8 | 0.2 |
| Hyl | 0.3 | 0.7 | 0.1 | |||
| Lys | 19.4 | 20.9 | 19.6 | 19.6 | 21.1 | 21.7 |
| Arg | 1.0 | 1.0 | 1.2 | 0.5 | 0.7 | 0.2 |
| total | 100 | 100 | 100 | 100 | 100 | 100 |
a
Actual represents measured composition after protein hydrolysis and amino acid analysis (n = 3, sd ±15%), whereas virtual denotes composition calculated from the cDNA deduced sequences of mcfp-1 variant 1, mgfp-1, and mefp-1.