Abstract
The diffusion-in-gel enzyme-linked immunosorbent assay (DIG-ELISA) is a new and simple method for quantitation of antibodies, based on the ability of antibodies to diffuse from wells in gel and adsorb to antigen which is bound to a polystyrene surface. The antigen-antibody reaction is visualized with a color reaction caused by horseradish peroxidase-conjugated class-specific anti-immunoglobins. This method was used to study the immunoglobulin G, A, and M immune response to Salmonella typhi O antigen in individuals immunized with a monovalent heat-inactivated typhoid vaccine. The antibody values obtained by the DIG-ELISA method correlated with those evaluated by conventional direct agglutination (Widal) and indirect hemagglutination methods. The DIG-ELISA method was also found to be sensitive, specific, and economical, as well as suitable for handling large numbers of sera while requiring very simple equipment.
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Selected References
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- Brash J. L., Lyman D. J. Adsorption of plasma proteins in solution to uncharged, hydrophobic polymer surfaces. J Biomed Mater Res. 1969 Mar;3(1):175–189. doi: 10.1002/jbm.820030114. [DOI] [PubMed] [Google Scholar]
- Elwing H., Nilsson L. A., Ouchterlony O. A simple spot technique for thin layer immunoassays (TIA) on plastic surfaces. J Immunol Methods. 1977;17(1-2):131–145. doi: 10.1016/0022-1759(77)90084-9. [DOI] [PubMed] [Google Scholar]
- Elwing H., Nilsson L., Ouchterlony O. Visualization principles in thin-layer immunoassays (TIA) on plastic surfaces. Int Arch Allergy Appl Immunol. 1976;51(6):757–762. doi: 10.1159/000231654. [DOI] [PubMed] [Google Scholar]
- Elwing H., Nygren H. Diffusion in gel-enzyme linked immunosorbent assay (DIG-ELISA): a simple method for quantitation of class-specific antibodies. J Immunol Methods. 1979;31(1-2):101–107. doi: 10.1016/0022-1759(79)90290-4. [DOI] [PubMed] [Google Scholar]
- Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
- Holmgren J., Svennerholm A. M. Enzyme-linked immunosorbent assays for cholera serology. Infect Immun. 1973 May;7(5):759–763. doi: 10.1128/iai.7.5.759-763.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Iwarson S., Larsson P. Intradermal versus subcutaneous immunization with typhoid vaccine. J Hyg (Lond) 1980 Feb;84(1):11–16. doi: 10.1017/s0022172400026462. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jeansson S., Elwing H., Nilsson L. A. Thin-layer immunoassay for determination of antibodies to herpes simplex virus. J Clin Microbiol. 1979 Mar;9(3):317–322. doi: 10.1128/jcm.9.3.317-322.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jodal U. The immune response to urinary tract infections in childhood. I. Serological diagnosis of primary symptomatic infection in girls by indirect hemagglutination. Acta Paediatr Scand. 1975 Jan;64(1):96–104. [PubMed] [Google Scholar]
- NETER E. Bacterial hemagglutination and hemolysis. Bacteriol Rev. 1956 Sep;20(3):166–188. doi: 10.1128/br.20.3.166-188.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nygren H., Hansson H. A., Lange S. Studies on the conjugation of horseradish peroxidase to immunoglobulin G via glutaraldehyde. Med Biol. 1979 Jun;57(3):187–191. [PubMed] [Google Scholar]
- Schroeder S. A. Interpretation of serologic tests for typhoid fever. JAMA. 1968 Oct 21;206(4):839–840. [PubMed] [Google Scholar]