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. 1980 Dec;12(6):748–752. doi: 10.1128/jcm.12.6.748-752.1980

Comparison of modified New York City medium with Martin-Lewis Medium for recovery of Neisseria gonorrhoeae from clinical specimens.

P A Granato, J L Paepke, L B Weiner
PMCID: PMC273690  PMID: 6796599

Abstract

A modified formulation of New York City medium was comparatively evaluated with Martin-Lewis medium for the recovery of Neisseria gonorrhoeae from clinical specimens. A total of 240 strains of gonococci were recovered from 1,250 specimens collected from walk-in patients attending a sexually transmitted disease clinic. N. gonorrhoeae was cultivated on both of these media from 182 clinical specimens with an additional 58 gonococcal strains isolated on either of the media. Of these discrepant gonococcal isolates, 27 strains were recovered on only modified New York City medium, whereas the remaining 31 strains were recovered on only Martin-Lewis agar. The differences in these isolation rates were not statistically significant. The overall results showed that modified New York City and Martin-Lewis media were comparable in their ability to grow gonococci from clinical material. Since modified New York City medium is capable of supporting the growth of N. gonorrhoeae, Mycoplasma pneumoniae, and urogenital mycoplasmas and inhibiting the growth of commensal microorganisms, it is possible that it may have considerable application as a multifunctional plating medium within the clinical laboratory.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brorson J. E., Holmberg I., Nygren B., Seeberg S. Vancomycin-sensitive strains of Neisseria gonorrhoeae. A problem for the diagnostic laboratory. Br J Vener Dis. 1973 Oct;49(5):452–453. doi: 10.1136/sti.49.5.452. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chen N. C., Hipp S. S., Lawton W. D., Gaafar H. A. Gonogrow, an improved, selective medium for the isolation of Neisseria gonorrhoeae. Health Lab Sci. 1974 Jul;11(3):173–177. [PubMed] [Google Scholar]
  3. Cross R. C., Hoger M. B., Neibaur R., Pasternack B., Brady F. J. VCN--inhibited strains of Neisseria gonorrhoeae. HSMHA Health Rep. 1971 Nov;86(11):990–992. [PMC free article] [PubMed] [Google Scholar]
  4. Faur Y. C., Weisburd M. H., Wilson M. E. A new medium for the isolation of pathogenic Neisseria (NYC medium). II. Effect of amphotericin B and trimethoprim lactate on selectivity. Health Lab Sci. 1973 Apr;10(2):55–60. [PubMed] [Google Scholar]
  5. Faur Y. C., Weisburd M. H., Wilson M. E., May P. S. A new medium for the isolation of pathogenic Neisseria (NYC medium). I. Formulation and comparisons with standard media. Health Lab Sci. 1973 Apr;10(2):44–54. [PubMed] [Google Scholar]
  6. Faur Y. C., Weisburd M. H., Wilson M. E. The selectivity of vancomycin and lincomycin in NYC medium for the recovery of N. gonorrhoeae from clinical specimens. Health Lab Sci. 1978 Jan;15(1):22–27. [PubMed] [Google Scholar]
  7. Faur Y. C., Weisburd M. H., Wlson M. E., May P. S. NYC medium for simultaneous isolation of Neisseria gonorrhoeae, large-colony mycoplasmas, and T-mycoplasmas. Appl Microbiol. 1974 Jun;27(6):1041–1045. doi: 10.1128/am.27.6.1041-1045.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Granato P. A., Poe L., Weiner L. B. Use of modified New York City medium for growth of Mycoplasma pneumoniae. A preliminary report. Am J Clin Pathol. 1980 May;73(5):702–705. doi: 10.1093/ajcp/73.5.702. [DOI] [PubMed] [Google Scholar]
  9. Martin J. E., Armstrong J. H., Smith P. B. New system for cultivation of Neisseria gonorrhoeae. Appl Microbiol. 1974 Apr;27(4):802–805. doi: 10.1128/am.27.4.802-805.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Martin J. E., Jr, Lester A. Transgrow, a medium for transport and growth of Neisseria gonorrhoeae and Neisseria meningitidis. HSMHA Health Rep. 1971 Jan;86(1):30–33. [PMC free article] [PubMed] [Google Scholar]
  11. Odegaard K., Solberg O., Lind J., Myhre G., Nyland B. Lincomycin in selective medium for the isolation of Neisseria gonorrhoeae. Acta Pathol Microbiol Scand B. 1975 Jun;83(3):301–304. doi: 10.1111/j.1699-0463.1975.tb00106.x. [DOI] [PubMed] [Google Scholar]
  12. Odegaard K. Trimethoprim for the prevention of overgrowth by swarming Proteus in the cultivation of gonococci. Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;79(4):545–548. [PubMed] [Google Scholar]
  13. Reyn A., Bentzon M. W. Comparison of a selective and a non-selective medium in the diagnosis of gonorrhoea to ascertain the sensitivity of Neisseria gonorrhoeae to vancomycin. Br J Vener Dis. 1972 Oct;48(5):363–368. doi: 10.1136/sti.48.5.363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Reyn A. Recent developments in the laboratory diagnosis of gonococcal infections. Bull World Health Organ. 1969;40(2):245–255. [PMC free article] [PubMed] [Google Scholar]
  15. Riddell R. H., Buck A. C. Trimethoprim as an additional selective agent in media for the isolation of N. gonorrhoeae. J Clin Pathol. 1970 Sep;23(6):481–483. doi: 10.1136/jcp.23.6.481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Seth A. Use of trimethoprim to prevent overgrowth by Proteus in the cultivation of N. gonorrhoeae. Br J Vener Dis. 1970 Jun;46(3):201–202. doi: 10.1136/sti.46.3.201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Symington D. A. An evaluation of New York City transport medium for the detection of Neisseria gonorrhoeae in clinical specimens. Health Lab Sci. 1975 Apr;12(2):69–75. [PubMed] [Google Scholar]
  18. Symington D. A. Improved transport system for Neisseria gonorrhoeae in clinical specimens. J Clin Microbiol. 1975 Dec;2(6):498–503. doi: 10.1128/jcm.2.6.498-503.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. THAYER J. D., MARTIN J. E., Jr A SELECTIVE MEDIUM FOR THE CULTIVATION OF N. GONORRHOEAE AND N. MENINGITIDIS. Public Health Rep. 1964 Jan;79:49–57. [PMC free article] [PubMed] [Google Scholar]
  20. Thayer J. D., Martin J. E., Jr Improved medium selective for cultivation of N. gonorrhoeae and N. meningitidis. Public Health Rep. 1966 Jun;81(6):559–562. [PMC free article] [PubMed] [Google Scholar]
  21. Whitbeck E., Shemonsky N., Levison M. E. Superiority of amphotericin over nystatin in Thayer-Martin medium. Antimicrob Agents Chemother. 1975 May;7(5):658–660. doi: 10.1128/aac.7.5.658. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Young H. Cultural diagnosis of gonorrhoea with modified New York City (MNYC) medium. Br J Vener Dis. 1978 Feb;54(1):36–40. doi: 10.1136/sti.54.1.36. [DOI] [PMC free article] [PubMed] [Google Scholar]

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