Abstract
An immunofluorescent slide method incorporating 0.1% (wt/vol) Evans blue as a counterstain was developed and compared with a recently described tube method. Seventy-one urine specimens were tested concurrently by both methods. Results of the two methods agreed in 70 specimens and disagreed in only 1. We found the slide method to be less costly and time consuming than the tube method.
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Selected References
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- Closs O., Aarli J. A. Evans blue as counterstain in the demonstration of muscle antibodies by immunofluorescence in myasthenia gravis. J Clin Pathol. 1974 Feb;27(2):162–167. doi: 10.1136/jcp.27.2.162. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Everett E. D., Eickhoff T. C., Ehret J. M. Immunofluorescence of yeast in urine. J Clin Microbiol. 1976 Aug;2(2):142–143. [PMC free article] [PubMed] [Google Scholar]
- Forsum U., Hjelm E., Jonsell G. Antibody-coated bacteria in the urine of children with urinary tract infections. Acta Paediatr Scand. 1976 Sep;65(5):639–642. doi: 10.1111/j.1651-2227.1976.tb04944.x. [DOI] [PubMed] [Google Scholar]
- Gonick P., Falkner B., Schwartz A., Pariser R. Bacteriuria in the catheterized patient. Cystitis or pyelonephritis? JAMA. 1975 Jul 21;233(3):253–255. [PubMed] [Google Scholar]
- Harding G. K., Marrie T. J., Ronald A. R., Hoban S., Muir P. Urinary tract infection localization in women. JAMA. 1978 Sep 8;240(11):1147–1151. [PubMed] [Google Scholar]
- Harding S. A., Merz W. G. Evaluation of antibody coating of yeasts in urine as an indicator of the site of urinary tract infection. J Clin Microbiol. 1975 Sep;2(3):222–225. doi: 10.1128/jcm.2.3.222-225.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harris R. E. Infections of the urinary tract during pregnancy: use of fluorescent antibody technic as an aid in patient evaluation. South Med J. 1976 Nov;69(11):1429–1432. doi: 10.1097/00007611-197611000-00010. [DOI] [PubMed] [Google Scholar]
- Hellerstein S., Kennedy E., Nussbaum L., Rice K. Localization of the site of urinary tract infections by means of antibody-coated bacteria in the urinary sediments. J Pediatr. 1978 Feb;92(2):188–193. doi: 10.1016/s0022-3476(78)80002-x. [DOI] [PubMed] [Google Scholar]
- Jones S. R. Antibody-coated bacteria in urine. N Engl J Med. 1976 Dec 9;295(24):1380–1380. doi: 10.1056/NEJM197612092952414. [DOI] [PubMed] [Google Scholar]
- Jones S. R., Johnson J. Further evaluation of the test for detection of antibody-coated bacteria in urine sediment. J Clin Microbiol. 1977 May;5(5):510–513. doi: 10.1128/jcm.5.5.510-513.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jones S. R. Letter: Prostatitis as cause of antibody-coated bacteria in urine. N Engl J Med. 1974 Aug 15;291(7):365–365. doi: 10.1056/NEJM197408152910714. [DOI] [PubMed] [Google Scholar]
- Jones S. R., Smith J. W., Sanford J. P. Localization of urinary-tract infections by detection of antibody-coated bacteria in urine sediment. N Engl J Med. 1974 Mar 14;290(11):591–593. doi: 10.1056/NEJM197403142901103. [DOI] [PubMed] [Google Scholar]
- Kulasinghe H. P., Cushing A. H., Reed W. P. Role of antibody-coated bacteria in the management of urinary tract infections. South Med J. 1977 Nov;70(11):1270–1272. doi: 10.1097/00007611-197711000-00006. [DOI] [PubMed] [Google Scholar]
- NICHOLS R. L., McCOMB D. E. Immunofluorescent studies with trachoma and related antigens. J Immunol. 1962 Oct;89:545–554. [PubMed] [Google Scholar]
- Pujol A., Linares F., Muñoz J., Rusconi A., Orejas V., Serrallach N., Acomar P. A new method for detection and localization of urinary infection. The fluorescence antibody test. Urol Int. 1976;31(6):501–507. doi: 10.1159/000280085. [DOI] [PubMed] [Google Scholar]
- RODRIGUEZ J., DEINHARDT F. Preparation of a semipermanent mounting medium for fluorescent antibody studies. Virology. 1960 Oct;12:316–317. doi: 10.1016/0042-6822(60)90205-1. [DOI] [PubMed] [Google Scholar]
- Thomas V., Shelokov A., Forland M. Antibody-coated bacteria in the urine and the site of urinary-tract infection. N Engl J Med. 1974 Mar 14;290(11):588–590. doi: 10.1056/NEJM197403142901102. [DOI] [PubMed] [Google Scholar]
