Abstract
The method of passive immune hemolysis of Evans and Evans (Infect. Immun. 16:604-609, 1977) for detection of heat-labile enterotoxin produced by enterotoxigenic Escherichia coli was modified. A total of 373 strains of E. coli were tested by this method using materials obtained by treating the cells with polymyxin B and rabbit antiserum against cholera enterotoxin, purified by affinity gel column coupled with purified cholera enterotoxin, in N-hydroxyethylpiperazine-N'-2-ethanesulfonic acid buffer (pH 6.7). The results correlated very well with those obtained in an assay with Chinese hamster ovary cells. It is concluded that passive immune hemolysis is useful as a routine clinical method for identifying E. coli strains that produce heat-labile enterotoxin.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Clements J. D., Finkelstein R. A. Isolation and characterization of homogeneous heat-labile enterotoxins with high specific activity from Escherichia coli cultures. Infect Immun. 1979 Jun;24(3):760–769. doi: 10.1128/iai.24.3.760-769.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Evans D. J., Jr, Evans D. G. Direct serological assay for the heat-labile enterotoxin of Escherichia coli, using passive immune hemolysis. Infect Immun. 1977 May;16(2):604–609. doi: 10.1128/iai.16.2.604-609.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Evans D. J., Jr, Evans D. G., Gorbach S. L. Production of vascular permeability factor by enterotoxigenic Escherichia coli isolated from man. Infect Immun. 1973 Nov;8(5):725–730. doi: 10.1128/iai.8.5.725-730.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Greenberg H. B., Sack D. A., Rodriguez W., Sack R. B., Wyatt R. G., Kalica A. R., Horswood R. L., Chanock R. M., Kapikian A. Z. Microtiter solid-phase radioimmunoassay for detection of Escherichia coli heat-labile enterotoxin. Infect Immun. 1977 Sep;17(3):541–545. doi: 10.1128/iai.17.3.541-545.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Guerrant R. L., Brunton L. L., Schnaitman T. C., Rebhun L. I., Gilman A. G. Cyclic adenosine monophosphate and alteration of Chinese hamster ovary cell morphology: a rapid, sensitive in vitro assay for the enterotoxins of Vibrio cholerae and Escherichia coli. Infect Immun. 1974 Aug;10(2):320–327. doi: 10.1128/iai.10.2.320-327.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Honda T., Shimizu M., Takeda Y., Miwatani T. Isolation of a factor causing morphological changes of chinese hamster ovary cells from the culture filtrate of Vibrio parahaemolyticus. Infect Immun. 1976 Oct;14(4):1028–1033. doi: 10.1128/iai.14.4.1028-1033.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moon H. W., Whipp S. C., Engstrom G. W., Baetz A. L. Response of the rabbit ileal loop to cell-free products from Escherichia coli enteropathogenic for swine. J Infect Dis. 1970 Feb;121(2):182–187. doi: 10.1093/infdis/121.2.182. [DOI] [PubMed] [Google Scholar]
- Mundell D. H., Anselmo C. R., Wishnow R. M. Factors influencing heat-labile Escherichia coli enterotoxin activity. Infect Immun. 1976 Aug;14(2):383–388. doi: 10.1128/iai.14.2.383-388.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sack D. A., Sack R. B. Test for enterotoxigenic Escherichia coli using Y-1 adrenal cells in miniculture. Infect Immun. 1975 Feb;11(2):334–336. doi: 10.1128/iai.11.2.334-336.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Serafim M. B., Pestana de Castro A. F., Lemos dos Reis M. H., Trabulsi L. R. Passive immune hemolysis for detection of heat-labile enterotoxin produced by Escherichia coli isolated from different sources. Infect Immun. 1979 Jun;24(3):606–610. doi: 10.1128/iai.24.3.606-610.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]