Abstract
A total of 1,013 isolates of Enterobacteriaceae were identified in parallel by the Repliscan (Cathra International, Ontario, Canada) and API 20E (Analytab Products, Plainview, N.Y.) systems. There was a 62% agreement at the genus level between the two systems. Of the 38% discrepant results, Repliscan classified 22% as "biochemical pattern not on file," 8% as a multiple-genus group which included the API 20E identification, and 8% as a genus other than that designated by API 20E. Relative to the various genera, Repliscan agreed with API 20E as follows: Escherichia coli, 80%; Klebsiella spp., 76%; Citrobacter spp., 75%; Proteus spp., 69%; Providencia spp., 54%; Serratia spp., 49%; Enterobacter spp., 25%; Shigella spp., 4%; and Salmonella spp., 0%. Repliscan identified 35% of Enterobacter spp. isolates as Citrobacter spp., 91% of Shigella spp. isolates as a multiple-choice-genus group, and 67% of Salmonella spp. isolates as "biochemical pattern not on file." Repliscan agreed with API 20E at the species level as follows: E. coli, 80%; Klebsiella spp., 56%; Citrobacter spp., 66%; Proteus spp., 55%; Providencia spp., 46%; Serratia spp., 39%; Enterobacter spp., 18%; Shigella spp., 4%; and Salmonella spp., 0%. These findings indicate that the Repliscan system in its present stage of development does not reliably identify the Enterobacteriaceae.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Aldridge K. E., Gardner B. B., Clark S. J., Matsen J. M. Comparison of micro-ID, API 20E, and conventional media systems in identification of Enterobacteriaceae. J Clin Microbiol. 1978 Jun;7(6):507–513. doi: 10.1128/jcm.7.6.507-513.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Barry A. L., Badal R. E. Rapid identification of Enterobacteriaceae with the micro-ID system versus API 20E and conventional media. J Clin Microbiol. 1979 Sep;10(3):293–298. doi: 10.1128/jcm.10.3.293-298.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Blazevic D. J., Mackay D. L., Warwood N. M. Comparison of Micro-ID and API 20E systems for identification of Enterobacteriaceae. J Clin Microbiol. 1979 May;9(5):605–608. doi: 10.1128/jcm.9.5.605-608.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brown S. D., Washington J. A., 2nd Evaluation of the Repliscan system for identification of Enterobacteriaceae. J Clin Microbiol. 1978 Dec;8(6):695–699. doi: 10.1128/jcm.8.6.695-699.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Butler D. A., Lobregat C. M., Gavan T. L. Reproducibility of the analytab (API 20E) system. J Clin Microbiol. 1975 Oct;2(4):322–326. doi: 10.1128/jcm.2.4.322-326.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Edberg S. C., Atkinson B., Chambers C., Moore M. H., Palumbo L., Zorzon C. F., Singer J. M. Clinical evaluation of the MICRO-ID, API 20E, and conventional media systems for identification of Enterobacteriacea. J Clin Microbiol. 1979 Aug;10(2):161–167. doi: 10.1128/jcm.10.2.161-167.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hayek L. J., Willis G. W. A comparison of two commerical methods for the identification of the Enterobacteriaceae--API 20E and the Enterotube--with conventional methods. J Clin Pathol. 1976 Feb;29(2):158–161. doi: 10.1136/jcp.29.2.158. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Marymont J. H., 3rd, Marymont J. H., Jr, Gavan T. L. Performance of enterobacteriaceae identification systems. An analysis of College of American Pathologists Survey data. Am J Clin Pathol. 1978 Sep;70(3 Suppl):539–547. [PubMed] [Google Scholar]
- Rutherford I., Moody V., Gavan T. L., Ayers L. W., Taylor D. L. Comparative study of three methods of identification of Enterobacteriaceae. J Clin Microbiol. 1977 Apr;5(4):458–464. doi: 10.1128/jcm.5.4.458-464.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tomfohrde K. M., Rhoden D. L., Smith P. B., Balows A. Evaluation of the redesigned enterotube--a system for the identification of Enterobacteriaceae. Appl Microbiol. 1973 Feb;25(2):301–304. doi: 10.1128/am.25.2.301-304.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Washington J. A., 2nd, Yu P. K., Martin W. J. Evaluation of accuracy of multitest micromethod system for identification of Enterobacteriaceae. Appl Microbiol. 1971 Sep;22(3):267–269. doi: 10.1128/am.22.3.267-269.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]