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. 1981 Jan;13(1):62–65. doi: 10.1128/jcm.13.1.62-65.1981

Analysis of tests used to differentiate Trichophyton rubrum from Trichophyton mentagrophytes.

J T Sinski, D Van Avermaete, L M Kelley
PMCID: PMC273722  PMID: 7007431

Abstract

Differential identification of Trichophyton rubrum and Trichophyton mentagrophytes was evaluated by using the standard in vitro hair perforation test as a criterion for identification and comparing its effectiveness with that of nine other tests used to differentiate these two taxa. Correlation and multiple regression analysis showed that no single test or combination of these tests was able to make the differentiation as effectively as the standard in vitro hair perforation test. Using relative error analysis, we suggest an identification scheme based upon selective utilization of potato-carrot agar for sporulation and the standard in vitro hair perforation test. Using potato-carrot agar for development of typical microconidia on each of the nonsporulating primary isolates and subsequently performing the standard in vitro hair perforation test only on those isolates that failed to produce spores on potato-carrot medium, we showed that this combination scheme provides more expedient results, without any sacrifice in reliability, than the standard in vitro hair perforation test alone.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AJELLO L., GEORG L. K. In vitro hair cultures for differentiating between atypical isolates of Trichophyton mentagrophytes and Trichophyton rubrum. Mycopathol Mycol Appl. 1957 Mar 25;8(1):3–17. doi: 10.1007/BF02053114. [DOI] [PubMed] [Google Scholar]
  2. Barry A. L., Fay G. D. A review of some common sources of error in the preparation of agar media. Am J Med Technol. 1972 Jul;38(7):241–245. [PubMed] [Google Scholar]
  3. Christensen W. B. Urea Decomposition as a Means of Differentiating Proteus and Paracolon Cultures from Each Other and from Salmonella and Shigella Types. J Bacteriol. 1946 Oct;52(4):461–466. doi: 10.1128/jb.52.4.461-466.1946. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DYSON J. E., Jr, LANDAY M. E. DIFFERENTIATION OF TRICHOPHYTON RUBRUM FROM TRICHOPHYTON MENTAGROPHYTES. Mycopathol Mycol Appl. 1963 Aug 31;20:81–97. doi: 10.1007/BF02054880. [DOI] [PubMed] [Google Scholar]
  5. FRANKS A. C., MASKIN I. L., TASCHDJIAN C. L. The etiology of dermatophytosis; shift from Trichophyton mentagrophytes to Trichophyton rubrum, 1935-1954. AMA Arch Derm. 1957 Jan;75(1):66–69. [PubMed] [Google Scholar]
  6. GEORG L. K., CAMP L. B. Routine nutritional tests for the identification of dermatophytes. J Bacteriol. 1957 Aug;74(2):113–121. doi: 10.1128/jb.74.2.113-121.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kane J., Fischer J. B. The differentiation of Trichophyton rubrum and T. mentagrophytes by use of Christensen's urea broth. Can J Microbiol. 1971 Jul;17(7):911–913. doi: 10.1139/m71-145. [DOI] [PubMed] [Google Scholar]
  8. LU Y. C. A new method for the study of hair digestion by dermatophytes. Mycopathol Mycol Appl. 1962 Jul 30;17:225–235. doi: 10.1007/BF02279295. [DOI] [PubMed] [Google Scholar]
  9. Littman M. L. A Culture Medium for the Primary Isolation of Fungi. Science. 1947 Aug 1;106(2744):109–111. doi: 10.1126/science.106.2744.109-a. [DOI] [PubMed] [Google Scholar]
  10. Philpot C. M. The use of nutritional tests for the differentiation of dermatophytes. Sabouraudia. 1977 Jul;15(2):141–150. doi: 10.1080/00362177785190221. [DOI] [PubMed] [Google Scholar]
  11. Rosenthal S. A., Fine H. L., Baer R. L. Present-day incidence of superficially infecting pathogenic fungi. Species identified in a diagnostic laboratory of mycology between 1964 and 1966. Arch Dermatol. 1967 Jul;96(1):51–52. [PubMed] [Google Scholar]
  12. SUDMAN M. S., SCHMITT J. A. DIFFERENTIATION OF TRICHOPHYTON RUBRUM AND TRICHOPHYTON MENTAGROPHYTES BY PIGMENT PRODUCTION. Appl Microbiol. 1965 Mar;13:290–290. doi: 10.1128/am.13.2.290-290.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Turner W. E., Kaplan W. The development and evaluation of immunodiffusion, immunofluorescence and physiological tests for the differentiation of atypical Trichophyton rubrum and T. mentagrophytes isolates. Mycopathol Mycol Appl. 1974 Aug 30;53(1):183–200. doi: 10.1007/BF02127208. [DOI] [PubMed] [Google Scholar]

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