Abstract
The efficiency of embryonated eggs and primary monkey kidney cell culture for isolation of contemporary influenza viruses was compared over three seasons by parallel inoculation of clinical materials. For the type A (H1N1) strains of 1977-1978 and for type B strains eggs were very inefficient, and nearly all viruses were isolated in cell culture alone. For the type A (H1N1) strains of 1978-1979 and for type A (H3N2) strains, isolations were more frequently made in eggs, but even here eggs were not as efficient for primary isolation as were cell cultures. It is concluded that primary monkey kidney cell cultures can be used as the single system for primary isolation of current type A and B influenza viruses.
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- Frank A. L., Couch R. B., Griffis C. A., Baxter B. D. Comparison of different tissue cultures for isolation and quantitation of influenza and parainfluenza viruses. J Clin Microbiol. 1979 Jul;10(1):32–36. doi: 10.1128/jcm.10.1.32-36.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KALTER S. S., CASEY H. L., JENSEN K. E., ROBINSON R. Q., GORRIE R. H. Evaluation of laboratory diagnostic procedures with A/Asian influenza. Proc Soc Exp Biol Med. 1959 Feb;100(2):367–370. doi: 10.3181/00379727-100-24629. [DOI] [PubMed] [Google Scholar]
- LENNETTE E. H. Laboratory diagnosis of influenza by virus isolation. Am Rev Respir Dis. 1961 Feb;83(2):116–119. doi: 10.1164/arrd.1961.83.2P2.116. [DOI] [PubMed] [Google Scholar]
- Meguro H., Bryant J. D., Torrence A. E., Wright P. F. Canine kidney cell line for isolation of respiratory viruses. J Clin Microbiol. 1979 Feb;9(2):175–179. doi: 10.1128/jcm.9.2.175-179.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Monto A. S., Cavallaro J. J. The Tecumseh study of respiratory illness. II. Patterns of occurrence of infection with respiratory pathogens, 1965-1969. Am J Epidemiol. 1971 Sep;94(3):280–289. doi: 10.1093/oxfordjournals.aje.a121321. [DOI] [PubMed] [Google Scholar]
- Monto A. S., Napier J. A., Metzner H. L. The Tecumseh study of respiratory illness. I. Plan of study and observations on syndromes of acute respiratory disease. Am J Epidemiol. 1971 Sep;94(3):269–279. doi: 10.1093/oxfordjournals.aje.a121320. [DOI] [PubMed] [Google Scholar]