Abstract
An antimicrobial susceptibility test, a tow-tube broth dilution and disk elution method for Ureaplasma urealyticum, was modified to incorporate some of the standard procedures followed in traditional antimicrobial testing. The susceptibility pattern of the species was reevaluated by determining the effect of various antimicrobial agents on 21 vaginal isolates. All isolates were inhibited by tetracycline congeners (1 to 6 micrograms/ml) and killed by methenamine mandelate (0.6 mg/ml). All but one isolates were inhibited by erythromycin (0.4 to 3 micrograms/ml). Only eight isolates were inhibited by nalidixic acid (1 to 6 micrograms/ml), and seven were inhibited by nitrofurantoin (20 to 60 micrograms/ml), whereas all isolates were resistant to rifampin (1 microgram/ml) and trimethoprim-sulfamethoxazole (5 micrograms/ml). The in vitro technique described can readily be performed on individual patient isolates before the initiation of antimicrobial therapy.
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- Bowie W. R., Floyd J. F., Miller Y., Alexander E. R., Holmes J., Holmes K. K. Differential response of chlamydial and ureaplasma-associated urethritis to sulphafurazole (sulfisoxazole) and aminocyclitols. Lancet. 1976 Dec 11;2(7998):1276–1278. doi: 10.1016/s0140-6736(76)92034-1. [DOI] [PubMed] [Google Scholar]
- Braun P., Klein J. O., Kass E. H. Susceptibility of genital mycoplasmas to antimicrobial agents. Appl Microbiol. 1970 Jan;19(1):62–70. doi: 10.1128/am.19.1.62-70.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Coufalik E. D., Taylor-Robinson D., Csonka G. W. Treatment of nongonococcal urethritis with rifampicin as a means of defining the role of Ureaplasma urealyticum. Br J Vener Dis. 1979 Feb;55(1):36–43. doi: 10.1136/sti.55.1.36. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Evans R. T., Taylor-Robinson D. The incidence of tetracycline-resistant strains of Ureaplasma urealyticum. J Antimicrob Chemother. 1978 Jan;4(1):57–63. doi: 10.1093/jac/4.1.57. [DOI] [PubMed] [Google Scholar]
- Ford D. K., Smith J. R. Non-specific urethritis associated with a tetracycline-resistant T-mycoplasma. Br J Vener Dis. 1974 Oct;50(5):373–374. doi: 10.1136/sti.50.5.373. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kundsin R. B., Parreno A., Poulin S. Significance of appropriate techniques and media for isolation and identification of Ureaplasma urealyticum from clinical specimens. J Clin Microbiol. 1978 Oct;8(4):445–453. doi: 10.1128/jcm.8.4.445-453.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robertson J. A. Bromothymol blue broth: improved medium for detection of Ureaplasma urealyticum (T-strain mycoplasma). J Clin Microbiol. 1978 Feb;7(2):127–132. doi: 10.1128/jcm.7.2.127-132.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SCHNEIERSON S. S., AMSTERDAM D. A simplified tube procedure for the routine determination of bacterial sensitivity to antibiotics. Am J Clin Pathol. 1959 Jan;31(1):81–86. doi: 10.1093/ajcp/31.1_ts.81. [DOI] [PubMed] [Google Scholar]
- Shepard M. C., Combs R. S. Enhancement of Ureaplasma urealyticum growth on a differential agar medium (A7B) by a polyamine, putrescine. J Clin Microbiol. 1979 Dec;10(6):931–933. doi: 10.1128/jcm.10.6.931-933.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shepard M. C., Lunceford C. D., Baker R. L. T-strain Mycoplasma. Selective inhibition by erythromycin in vitro. Br J Vener Dis. 1966 Mar;42(1):21–24. doi: 10.1136/sti.42.1.21. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shepard M. C., Lunceford C. D. Differential agar medium (A7) for identification of Ureaplasma urealyticum (human T mycoplasmas) in primary cultures of clinical material. J Clin Microbiol. 1976 Jun;3(6):613–625. doi: 10.1128/jcm.3.6.613-625.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shepard M. C., Lunceford C. D. Serological typing of Ureaplasma urealyticum isolates from urethritis patients by an agar growth inhibition method. J Clin Microbiol. 1978 Nov;8(5):566–574. doi: 10.1128/jcm.8.5.566-574.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spaepen M. S., Kundsin R. B., Horne H. W. Tetracycline-resistant T-mycoplasmas (Ureaplasma urealyticum) from patients with a history of reproductive failure. Antimicrob Agents Chemother. 1976 Jun;9(6):1012–1018. doi: 10.1128/aac.9.6.1012. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spaepen M. S., Kundsin R. B. Simple, direct broth-disk method for antibiotic susceptibility testing of Ureaplasma urealyticum. Antimicrob Agents Chemother. 1977 Feb;11(2):267–270. doi: 10.1128/aac.11.2.267. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taylor-Robinson D., Csonka G. W., Prentice M. J. Human intra-urethral inoculation of ureplasmas. Q J Med. 1977 Jul;46(183):309–326. [PubMed] [Google Scholar]
- Taylor-Robinson D., McCormack W. M. The genital mycoplasmas (first of two parts). N Engl J Med. 1980 May 1;302(18):1003–1010. doi: 10.1056/NEJM198005013021805. [DOI] [PubMed] [Google Scholar]