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. 1981 Feb;13(2):369–379. doi: 10.1128/jcm.13.2.369-379.1981

Relationships between serological groups and deoxyribonucleic acid homology groups in Bacteroides fragilis and related species.

J L Babb, C S Cummings
PMCID: PMC273791  PMID: 6162856

Abstract

The serological properties of antigens extracted from strains of Bacteroides fragilis and related species belonging to several different deoxyribonucleic acid homology groups were investigated. Antisera prepared against Formalin-treated whole cell suspensions of representative strains were tested against cell suspensions, cell wall preparations, and extracts of homologous and heterologous strains by using agglutination, immunodiffusion, and hemagglutination techniques. Serological results indicated that the species were antigenically distinct, although minor cross-reactions were observed. Homology groups, including the two B. fragilis subgroups, were relatively homogeneous, although the presence of serotypes within each homology group was suggested. Immunodiffusion tests demonstrated, however, that each possessed a mosaic antigen composition; at least 6 antigenic determinants could be demonstrated in B. fragilis 2553, and up to 10 were found in B. fragilis 2393. Hemagglutination tests using antigen extracts also indicate a mosaic of antigens in each strain.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abshire R. L., Dowell V. R., Jr, Lombard G. L. Serological study of trichloroacetic acid extracts of Bacteroides fragilis. J Clin Microbiol. 1979 Feb;9(2):274–279. doi: 10.1128/jcm.9.2.274-279.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Babb J. L., Cummins C. S. Encapsulation of Bacteroides species. Infect Immun. 1978 Mar;19(3):1088–1091. doi: 10.1128/iai.19.3.1088-1091.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Beerens H., Wattre P., Shinjo T., Romond C. Premiers résultats d'un essai de classification sérologique de 131 souches de Bacteroïdes du groupe fragilis (eggerthella. Ann Inst Pasteur (Paris) 1971 Aug;121(2):187–198. [PubMed] [Google Scholar]
  4. Brenner D. J., Falkow S. Genetics of the Enterobacteriaceae. C. Molecular relationships among members of the Enterobacteriaceae. Adv Genet. 1971;16:81–118. doi: 10.1016/s0065-2660(08)60355-7. [DOI] [PubMed] [Google Scholar]
  5. Cherniak R., Lombard G. L., Dowell V. R., Jr Immunochemical evidence for multiple serotypes of Bacteroides fragilis. J Clin Microbiol. 1979 Jun;9(6):699–704. doi: 10.1128/jcm.9.6.699-704.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cummins C. S. Identification of Propionibacterium acnes and related organisms by precipitin tests with trichloroacetic acid extracts. J Clin Microbiol. 1976 Aug;2(2):104–110. [PMC free article] [PubMed] [Google Scholar]
  7. Elhag K. M., Bettelheim K. A., Tabaqchali S. Serological studies of Bacteroides fragilis. J Hyg (Lond) 1977 Oct;79(2):233–241. doi: 10.1017/s0022172400053043. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Elhag K. M., Tabaqchali S. A study of the surface and somatic antigens of Bacteroides fragilis. J Hyg (Lond) 1978 Jun;80(3):439–449. doi: 10.1017/s0022172400024918. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hartwigk H. Uber das Vorkommen von Bacteroides thetaiotaomicron beim Tier. Zentralbl Bakteriol Orig A. 1973 Feb;223(2):211–219. [PubMed] [Google Scholar]
  10. Hofstad T. O-antigenic specificity of lipopolysaccharides from Bacteroides fragilis ss. fragilis. Acta Pathol Microbiol Scand B. 1975 Oct;83(5):477–481. doi: 10.1111/j.1699-0463.1975.tb00128.x. [DOI] [PubMed] [Google Scholar]
  11. Hofstad T. Serological responses to antigens of Bacteroidaceae. Microbiol Rev. 1979 Mar;43(1):103–115. doi: 10.1128/mr.43.1.103-115.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kasper D. L. Chemical and biological characterization of the lipopolysaccharide of Bacteroides fragilis subspecies fragilis. J Infect Dis. 1976 Jul;134(1):59–66. doi: 10.1093/infdis/134.1.59. [DOI] [PubMed] [Google Scholar]
  13. Kasper D. L., Hayes M. E., Reinap B. G., Craft F. O., Onderdonk A. B., Polk B. F. Isolation and identification of encapsulated strains of Bacteroides fragilis. J Infect Dis. 1977 Jul;136(1):75–81. doi: 10.1093/infdis/136.1.75. [DOI] [PubMed] [Google Scholar]
  14. Kasper D. L., Seiler M. W. Immunochemical characterization of the outer membrane complex of Bacteroides fragilis subspecies fragilis. J Infect Dis. 1975 Oct;132(4):440–450. doi: 10.1093/infdis/132.4.440. [DOI] [PubMed] [Google Scholar]
  15. Kasper D. L. The polysaccharide capsule of Bacteroides fragilis subspecies fragilis: immunochemical and morphologic definition. J Infect Dis. 1976 Jan;133(1):79–87. doi: 10.1093/infdis/133.1.79. [DOI] [PubMed] [Google Scholar]
  16. Lambe D. W., Jr, Moroz D. A. Serogrouping of Bacteroides fragilis subsp. fragilis by the agglutination test. J Clin Microbiol. 1976 Jun;3(6):586–592. doi: 10.1128/jcm.3.6.586-592.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Landay M. E., Baldwin W. Comparative serology of two clinical isolates of Bacteroides fragilis and Bacteroides thetaiotaomicron. J Clin Microbiol. 1979 May;9(5):643–644. doi: 10.1128/jcm.9.5.643-644.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Quick J. D., Goldberg H. S., Sonnenwirth A. C. Human antibody to Bacteroidaceae. Am J Clin Nutr. 1972 Dec;25(12):1351–1356. doi: 10.1093/ajcn/25.12.1351. [DOI] [PubMed] [Google Scholar]
  19. Reinhold L. Serologische Untersuchungen an Stämmen von Bacteroides thetaiotaomicron und Bacteroides fragilis im Agargelpräzipitationstest. Zentralbl Bakteriol Orig. 1971;216(2):219–227. [PubMed] [Google Scholar]
  20. Romond C., Beerens H., Wattre P. Identification sérologique des bactéroïdes en relation avec leur pouvoir pathogène. Arch Roum Pathol Exp Microbiol. 1972 Sep;31(3):351–355. [PubMed] [Google Scholar]
  21. Sharpe M. E. Serology of rumen bacteroides. J Gen Microbiol. 1971 Aug;67(3):273–288. doi: 10.1099/00221287-67-3-273. [DOI] [PubMed] [Google Scholar]
  22. Werner H. Das serologische Verhalten von Stämmen der Species Bacteroides convexus, B. thetaiotaomicron, B. vulgatus und B. distasonis. Zentralbl Bakteriol Orig. 1969 Jun;210(2):192–201. [PubMed] [Google Scholar]

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