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. 1981 Mar;13(3):405–409. doi: 10.1128/jcm.13.3.405-409.1981

Enzyme-linked immunosorbent assay for detection of immunoglobulin M antibody to hepatitis B core antigen.

P Kryger, L R Mathiesen, A M Møller, J Aldershvile, B G Hansson, J O Nielsen
PMCID: PMC273804  PMID: 7240384

Abstract

An enzyme-linked immunosorbent assay for detection of specific immunoglobulin M (IgM) antibodies against the core antigen of the hepatitis B virus (anti-HBc IgM) is described. The interference of IgM rheumatoid factor was evaluated quantitatively. In the anti-HBc IgM test, the rheumatoid factor gave false-positive results when the concentration exceeded 20 IU/ml. The rheumatoid-positive sera were disclosed by a control and retested for anti-HBc IgM after absorption of rheumatoid factor with latex particles aggregated with human IgG. In five of seven selected patients with acute hepatitis B followed to biochemical and clinical recovery, anti-HBc IgM was present transiently until antibodies against hepatitis B surface antigen (anti-HBs) appeared. Two patients had persistent anti-HBc IgM during the follow-up period. Four patients with hepatitis B surface antigenemia and progression to chronic liver disease did not clear their anti-HBc IgM in the period of observation (11 to 24 months). Anti-HBc IgM could not be demonstrated in 223 of 225 Danish blood donors. The two donors found positive for anti-HBc IgM also had anti-HBs. Twenty patients with acute A or non-A non-B hepatitis were negative for anti-HBc IgM. The enzyme-linked immunosorbent assay for anti-HBc IgM described here has a high specificity and sensitivity. The diagnostic relevance needs further evaluation, including quantitation of anti-HBc IgM, but the results presented indicate that anti-HBc IgM may be helpful in differentiating between prior and recent or ongoing hepatitis B infection.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cohen B. J. The IgM antibody responses to the core antigen of hepatitis B virus. J Med Virol. 1978;3(2):141–149. doi: 10.1002/jmv.1890030208. [DOI] [PubMed] [Google Scholar]
  2. Duermeyer W., Wielaard F., van der Veen J. A new principle for the detection of specific IgM antibodies applied in an ELISA for hepatitis A. J Med Virol. 1979;4(1):25–32. doi: 10.1002/jmv.1890040104. [DOI] [PubMed] [Google Scholar]
  3. Gerlich W. H., Lüer W. Selective detection of IgM-antibody against core antigen of the hepatitis B virus by a modified enzyme immune assay. J Med Virol. 1979;4(3):227–238. doi: 10.1002/jmv.1890040308. [DOI] [PubMed] [Google Scholar]
  4. Hansson B. G. Age- and sex-related distribution of antibodies to hepatitis B surface and core antigens in a Swedish population. Acta Pathol Microbiol Scand B. 1976 Dec;84B(6):342–346. doi: 10.1111/j.1699-0463.1976.tb01951.x. [DOI] [PubMed] [Google Scholar]
  5. Hoofnagle J. H., Gerety R. J., Ni L. Y., Barker L. F. Antibody to hepatitis B core antigen. A sensitive indicator of hepatitis B virus replication. N Engl J Med. 1974 Jun 13;290(24):1336–1340. doi: 10.1056/NEJM197406132902402. [DOI] [PubMed] [Google Scholar]
  6. Hoofnagle J. H., Seeff L. B., Bales Z. B., Zimmerman H. J. Type B hepatitis after transfusion with blood containing antibody to hepatitis B core antigen. N Engl J Med. 1978 Jun 22;298(25):1379–1383. doi: 10.1056/NEJM197806222982502. [DOI] [PubMed] [Google Scholar]
  7. Locarnini S. A., Coulepis A. G., Stratton A. M., Kaldor J., Gust I. D. Solid-phase enzyme-linked immunosorbent assay for detection of hepatitis A-specific immunoglobulin M. J Clin Microbiol. 1979 Apr;9(4):459–465. doi: 10.1128/jcm.9.4.459-465.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mathiesen L. R., Feinstone S. M., Wong D. C., Skinhoej P., Purcell R. H. Enzyme-linked immunosorbent assay for detection of hepatitis A antigen in stool and antibody to hepatitis A antigen in sera: comparison with solid-phase radioimmunoassay, immune electron microscopy, and immune adherence hemagglutination assay. J Clin Microbiol. 1978 Feb;7(2):184–193. doi: 10.1128/jcm.7.2.184-193.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Møller A. M., Mathiesen L. R. Detection of immunoglobulin M antibodies to hepatitis A virus by enzyme-linked immunosorbent assay. J Clin Microbiol. 1979 Nov;10(5):628–632. doi: 10.1128/jcm.10.5.628-632.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Niermeiier P., Gips C. H., Huizenga J. R., Ringers J., Verkerk S., Houthuff H. J., Houwen B., Snijder J. A., Nielsen J. O. IgM-anti-HBc as a marker of persistent and IgG-anti-HBc as a marker of past hepatitis B infection. A longitudinal study over 5 years. Acta Hepatogastroenterol (Stuttg) 1978 Oct;25(5):360–364. [PubMed] [Google Scholar]
  11. Omata M., Afroudakis A., Liew C. T., Ashcavai M., Peters R. L. Comparison of serum hepatitis B surface antigen (HBsAg) and serum anticore with tissue HBsAg and hepatitis B core antigen (HBcAg). Gastroenterology. 1978 Dec;75(6):1003–1009. [PubMed] [Google Scholar]
  12. Schrohenloher R. E. Copper-catalysed reoxidation of human monoclonal IgM. Scand J Immunol. 1978;8(5):443–449. doi: 10.1111/j.1365-3083.1978.tb00540.x. [DOI] [PubMed] [Google Scholar]
  13. Vejtorp M., Fanøe E., Leerhoy J. Diagnosis of postnatal rubella by the enzyme-linked immunosorbent assay for rubella IgM and IgG antibodies. Acta Pathol Microbiol Scand B. 1979 Jun;87B(3):155–160. doi: 10.1111/j.1699-0463.1979.tb02419.x. [DOI] [PubMed] [Google Scholar]
  14. Vejtorp M., Høier-Madsen M., Halberg P. Enzyme-linked immunosorbent assay for determination of IgM rheumatoid factor. Scand J Rheumatol. 1979;8(2):65–70. doi: 10.3109/03009747909105338. [DOI] [PubMed] [Google Scholar]
  15. Yolken R. H., Wyatt R. G., Kim H. W., Kapikian A. Z., Chanock R. M. Immunological response to infection with human reovirus-like agent: measurement of anti-human reovirus-like agent immunoglobulin G and M levels by the method of enzyme-linked immunosorbent assay. Infect Immun. 1978 Feb;19(2):540–546. doi: 10.1128/iai.19.2.540-546.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

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