Abstract
A newly developed commercial serological test (Iatron Laboratories, Inc., Tokyo, Japan) for the rapid identification of medically important species of Candida was evaluated against the API 20C (Analytab Products, Plainview, N.Y.) and the standard Wickerham assimilation and fermentation procedures. Our results indicated that the Iatron and the API 20C methods are 95% accurate since both permitted identification of 78 of 82 Candida isolates, representing eight medically important species. None of the tests on nine Cryptococcus, six Trichosporon, three Geotrichum, three Saccharomyces, and one Rhodotorula species yielded false-positive reactions. False-positive serological tests occurred with a species of Pichia and Candida rugosa. The API 20C procedure correctly identified C. rugosa but not the Pichia sp. The Iatron method permitted reliable identification of the Candida species in 10 min to 5 h, whereas the API 20C procedure required 48 to 72 h. Neither method could properly identify sucrose-negative Candida tropicalis or Candida lusitaniae isolates. In addition, Candida albicans isolates could be serotyped by the Iatron method.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ahearn D. G., Meyer S. A., Mitchell G., Nicholson M. A., Ibrahim A. I. Sucrose-negative variants of Candida tropicalis. J Clin Microbiol. 1977 Apr;5(4):494–496. doi: 10.1128/jcm.5.4.494-496.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bowman P. I., Ahearn D. G. Evaluation of commercial systems for the identification of clinical yeast isolates. J Clin Microbiol. 1976 Jul;4(1):49–53. doi: 10.1128/jcm.4.1.49-53.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bowman P. I., Ahearn D. G. Evaluation of the Uni-Yeast-Tek kit for the identification of medically important yeasts. J Clin Microbiol. 1975 Oct;2(4):354–358. doi: 10.1128/jcm.2.4.354-358.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Buesching W. J., Kurek K., Roberts G. D. Evaluation of the modified API 20C system for identification of clinically important yeasts. J Clin Microbiol. 1979 May;9(5):565–569. doi: 10.1128/jcm.9.5.565-569.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fukazawa Y., Shinoda T., Tsuchiya T. Response and specificity of antibodies for Candida albicans. J Bacteriol. 1968 Mar;95(3):754–763. doi: 10.1128/jb.95.3.754-763.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Holzschu D. L., Presley H. L., Miranda M., Phaff H. J. Identification of Candida lusitaniae as an opportunistic yeast in humans. J Clin Microbiol. 1979 Aug;10(2):202–205. doi: 10.1128/jcm.10.2.202-205.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Huppert M., Harper G., Sun S. H., Delanerolle V. Rapid methods for identification of yeasts. J Clin Microbiol. 1975 Jul;2(1):21–34. doi: 10.1128/jcm.2.1.21-34.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Land G. A., Harrison B. A., Hulme K. L., Cooper B. H., Byrd J. C. Evaluation of the new API 20C strip for yeast identification against a conventional method. J Clin Microbiol. 1979 Sep;10(3):357–364. doi: 10.1128/jcm.10.3.357-364.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TSUCHIYA T., FUKAZAWA Y., KAWAKITA S. A method for the rapid identification of the genus Candida. Mycopathol Mycol Appl. 1959 Mar 31;10(3):191–206. doi: 10.1007/BF02053014. [DOI] [PubMed] [Google Scholar]
- Tsuchiya T., Fukazawa Y., Kawakita S. Significance of serological studies on yeasts. Mycopathol Mycol Appl. 1965 Jun 15;26(1):1–15. doi: 10.1007/BF02098585. [DOI] [PubMed] [Google Scholar]
- Tsuchiya T., Fukazawa Y., Taguchi M., Nakase T., Shinoda T. Serologic aspects on yeast classification. Mycopathol Mycol Appl. 1974 Aug 30;53(1):77–91. doi: 10.1007/BF02127199. [DOI] [PubMed] [Google Scholar]