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. 1981 Apr;13(4):618–626. doi: 10.1128/jcm.13.4.618-626.1981

Immunoglobulin M antibodies to hepatitis B core antigen: evaluation of enzyme immunoassay for diagnosis of hepatitis B virus infection.

M Roggendorf, F Deinhardt, G G Frösner, R Scheid, B Bayerl, R Zachoval
PMCID: PMC273847  PMID: 6164690

Abstract

In acute and chronic hepatitis B, antibodies of the immunoglobulin M (IgM) class against the hepatitis B core antigen (anti-HBc IgM) have been demonstrated. For the determination of anti-HBc IgM, a sensitive enzyme immunoassay with anti-mu-coated flat-bottomed microtiter plates is described and evaluated. The specificity of the anti-HBc IgM test system was proven by pretreatment of presumed anti-HBc IgM-positive samples with anti-mu to block anti-HBc IgM. The test system was highly sensitive. In the acute stage of hepatitis B, anti-HBc IgM could be demonstrated in serum dilutions up to 10(-7) (mean titer, 10(-5)), and in sera from patients with chronic hepatitis B, the mean titer was 10(-3). In a study of unselected patients whose sera were sent at irregular intervals for testing, anti-HBc IgM persisted in a high percentage (52%) for at least 13 to 18 months after onset of illness despite the fact that these patients eliminated hepatitis B surface antigen (HBsAg) and produced antibodies to HBsAg (anti-HBs). By using the anti-HBc IgM test as an additional aid in the diagnosis of acute HBsAg-negative hepatitis, the hepatitis B etiology could be established in 13 of 42 patients (31.4%). Investigations of the prevalence of anti-HBc IgM in different groups of patients with chronic hepatitis B infection showed 89.4% anti-HBc IgM-positive results in patients with chronic active hepatitis B, 60% in patients with HBsAg-negative chronic active hepatitis, 58.2% in patients with primary liver carcinoma and markers of hepatitis B infections, and 34.9% in healthy carriers of HBsAg.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cohen B. J. The IgM antibody responses to the core antigen of hepatitis B virus. J Med Virol. 1978;3(2):141–149. doi: 10.1002/jmv.1890030208. [DOI] [PubMed] [Google Scholar]
  2. Dissanayake S., Hay F. C., Roitt I. M. The binding constants of IgM rheumatoid factors and their univalent fragments for native and aggregated human IgG;. Immunology. 1977 Mar;32(3):309–318. [PMC free article] [PubMed] [Google Scholar]
  3. Duermeyer W., van der Veen J. Specific detection of IgM-antibodies by ELISA, applied in hepatitis-A. Lancet. 1978 Sep 23;2(8091):684–685. doi: 10.1016/s0140-6736(78)92802-7. [DOI] [PubMed] [Google Scholar]
  4. Frösner G. G., Brodersen M., Papaevangelou G., Sugg U., Haas H., Mushahwar I. K., Ling C. M., Overby L. R., Deinhardt F. Detection of HBeAg and anti-HBe in acute hepatitis B by a sensitive radioimmunoassay. J Med Virol. 1978;3(1):67–76. doi: 10.1002/jmv.1890030114. [DOI] [PubMed] [Google Scholar]
  5. Gerlich W. H., Lüer W. Selective detection of IgM-antibody against core antigen of the hepatitis B virus by a modified enzyme immune assay. J Med Virol. 1979;4(3):227–238. doi: 10.1002/jmv.1890040308. [DOI] [PubMed] [Google Scholar]
  6. Hawkes R. A., Boughton C. R., Ferguson V., Lehmann N. I. Use of immunoglobulin M antibody to hepatitis B core antigen in diagnosis of viral hepatitis. J Clin Microbiol. 1980 Jun;11(6):581–583. doi: 10.1128/jcm.11.6.581-583.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Krech U., Wilhelm J. A. A solid-phase immunosorbent technique for the rapid detection of rubella IgM by haemagglutination inhibition. J Gen Virol. 1979 Aug;44(2):281–286. doi: 10.1099/0022-1317-44-2-281. [DOI] [PubMed] [Google Scholar]
  8. Pastore G., Zanetti A. R., Ferroni P., Dentico P., Angarano G., Schiraldi O. Radioimmunoassay in the detection of the hepatitis B e antigen/antibody system in asymptomatic carriers of hepatitis B surface antigen. Correlation with serum Dane particle associated DNA polymerase activity. Infection. 1979;7(6):279–282. doi: 10.1007/BF01642149. [DOI] [PubMed] [Google Scholar]
  9. Roggendorf M., Frösner G. G., Deinhardt F., Scheid R. Comparison of solid phase test systems for demonstrating antibodies against hepatitis A virus (anti-Hav) of the IgM-class. J Med Virol. 1980;5(1):47–62. doi: 10.1002/jmv.1890050107. [DOI] [PubMed] [Google Scholar]
  10. Schmitz H., von Deimling U., Flehmig B. Detection of IgM antibodies to cytomegalovirus (CMV) using an enzyme-labelled antigen (ELA). J Gen Virol. 1980 Sep;50(1):59–68. doi: 10.1099/0022-1317-50-1-59. [DOI] [PubMed] [Google Scholar]
  11. Siegert W., Grunst J., Wilmanns W., Frösner G. G., Deinhardt F. Quantitative correlation between the Dane particle-associated DNA polymerase and the hepatitis B e antigen. Infection. 1979;7(5):220–222. doi: 10.1007/BF01648929. [DOI] [PubMed] [Google Scholar]
  12. Szmuness W. Hepatocellular carcinoma and the hepatitis B virus: evidence for a causal association. Prog Med Virol. 1978;24:40–69. [PubMed] [Google Scholar]

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