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. 2009 Jun 29;114(9):1919–1928. doi: 10.1182/blood-2008-12-195180

Table 2.

Components of glycophorin/band 3 macrocomplexes identified and quantified by iTRAQ™

No. % Sequence Coverage Accession Name 115:114
116:114
117:114
Ratio P Ratio P Ratio P
1 33.83 P02549 SPTA1_HUMAN Spectrin α chain, erythrocyte 1.09 < .005 1.06 .003 1.11 < .005
2 30.93 P11277 SPTB1_HUMAN Spectrin β chain, erythrocyte 1.15 < .005 1.08 < .005 1.12 < .005
3 25.57 P16157 ANK1_HUMAN Ankyrin-1 (erythrocyte ankyrin) 1.08 .006 1.15 < .005 1.22 < .005
4 29.42 P02730 B3AT_HUMAN Band 3 anion transport protein (anion exchange protein 1) 0.97 .361 1.67 < .005 1.25 < .005
5 31.40 P16452 EPB42_HUMAN Erythrocyte membrane protein band 4.2 1.02 .797 1.14 .009 1.32 < .005
6 32.00 P60709 ACTB_HUMAN Actin, cytoplasmic* 1.21 .000 1.08 .101 1.26 < .005
7 27.16 Q08495 DEMA_HUMAN Dematin (erythrocyte membrane protein band 4.9) 1.20 .158 1.11 .211 1.00 > .999
8 24.07 P11171 41_HUMAN Protein 4.1 (band 4.1) 0.87 .002 1.08 .018 1.13 .016
9 29.51 P27105 STOM_HUMAN Stomatin (protein 7.2b) 0.98 .689 0.94 .141 1.10 .014
10 34.30 Q14254 FLOT2_HUMAN Flotillin-2 1.27 .026 1.02 .730 1.08 .372
11 9.35 P11166 GTR1_HUMAN Solute carrier family 2, facilitated glucose transporter member 1 0.83 .009 1.07 .060 1.26 .000
12 47.57 P62805 H4_HUMAN Histone H4 0.96 .520 0.51 .067 0.65 .021
13 13.73 P04406 G3P_HUMAN Glyceraldehyde-3-phosphate dehydrogenase 0.90 .420 0.94 .261 1.07 .158
14 26.46 O75955 FLOT1_HUMAN Flotillin-1 0.98 .794 0.89 .313 1.02 .689
15 22.45 P68871 HBB_HUMAN Hemoglobin 1.06 .859 1.06 .448 0.89 .289
16 27.78 Q99877 H2B1N_HUMAN Histone H2B 1.17 .302 0.33 .011 0.57 .020
17 56.58 P62988 UBIQ_HUMAN Ubiquitin 1.27 .151 1.22 .159 1.16 .200
18 30.77 Q6FI13 H2A2A_HUMAN Histone H2A 0.25 0.19 0.40
19 6.71 Q02161 RHD_HUMAN Blood group Rh§ 1.23 .659 1.00 > .999 1.54 .032
20 50.00 Q71DI3 H32_HUMAN Histone H3 1.17 .147 0.53 .003 0.60 .004
21 25.00 P06753 TPM3_HUMAN Tropomyosin α 1.00 > .999 0.97 .677 0.89 .356
22 6.51 P35611 ADDA_HUMAN α-Adducin 0.76 .127 1.20 .141 0.91 .419
23 2.60 P29972 AQP1_HUMAN AQP1 0.78 0.84 1.41
*

The β and γ isoforms could not be differentiated.

Multiple isoforms could not be differentiated.

The β and δ isoforms could not be differentiated.

§

Rh polypeptide 1 and Rh polypeptide 2 could not be differentiated.

The α-3 and α-4 isoforms could not be differentiated.