Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1981 Apr;13(4):677–680. doi: 10.1128/jcm.13.4.677-680.1981

Evaluation and optimization of urine screening by Autobac.

M T Kelly, L C Balfour
PMCID: PMC273858  PMID: 7014617

Abstract

The purpose of this investigation was to evaluate the effectiveness of the Autobac (Pfizer Inc., New York, N.Y.) urine screen for detection of bacteriuria in 3,026 urine specimens and to establish the optimum procedure for the Autobac system. Overall, 97% of urine specimens having greater than 10(5) colony-forming units (CFU) per ml were detected within 5 h by the Autobac system. The system detected 66, 90, and 94% of such specimens after 2, 3, and 4 h of incubation, respectively. Of specimens having 10(4) to 10(5) CFU/ml, the Autobac system detected 10, 45, 53, and 95% after 2, 3, 4, and 5 h of incubation, respectively. The rate of false-positive results increased from 0% after 2 h to 2% after 3 h to 6% after 4 h and 25% after 5 h of incubation. The specificity of the urine screening results also varied with the incubation time. Percentages of specimens having greater than 10(5) CFU/ml that gave positive urine screening results at various times were as follows: 96% at 2 h, 74% at 3 h, 29% at 4 h, and 9% at 5 h. These findings suggest that a 3- or 4-h urine screening procedure will effectively detect bacteriuria of greater than 10(5) CFU/ml, with few false-positive results. However, a 5-h procedure, which gives more false-positive results, may be needed for detection of lower levels of bacteriuria.

Full text

PDF
677

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Heinze P. A., Thrupp L. D., Anselmo C. R. A rapid (4--6-hour) urine-culture system for direct identification and direct antimicrobial susceptibility testing. Am J Clin Pathol. 1979 Feb;71(2):177–183. doi: 10.1093/ajcp/71.2.177. [DOI] [PubMed] [Google Scholar]
  2. Isenberg H. D., Gavan T. L., Sonnenwirth A., Taylor W. I., Washington J. A., 2nd Clinical laboratory evaluation of automated microbial detection/identification system in analysis of clinical urine specimens. J Clin Microbiol. 1979 Aug;10(2):226–230. doi: 10.1128/jcm.10.2.226-230.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Jenkins R. D., Hale D. C., Matsen J. M. Rapid semiautomated screening and processing of urine specimens. J Clin Microbiol. 1980 Mar;11(3):220–225. doi: 10.1128/jcm.11.3.220-225.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Nicholson D. P., Koepke J. A. The Automicrobic System for urines. J Clin Microbiol. 1979 Dec;10(6):823–833. doi: 10.1128/jcm.10.6.823-833.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Stamm W. E., Wagner K. F., Amsel R., Alexander E. R., Turck M., Counts G. W., Holmes K. K. Causes of the acute urethral syndrome in women. N Engl J Med. 1980 Aug 21;303(8):409–415. doi: 10.1056/NEJM198008213030801. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES