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. 1981 Apr;13(4):698–704. doi: 10.1128/jcm.13.4.698-704.1981

Diagnosis of recent rubella virus infection by demonstration of specific immunoglobulin M antibodies: comparison of solid-phase reverse immunosorbent test with sucrose density gradient centrifugation.

G A Denoyel, A Gaspar, D Peyramond
PMCID: PMC273862  PMID: 7014619

Abstract

A solid-phase reverse immunosorbent test (SPRIST) based on the addition of an excess of rubella virus hemagglutinin was evaluated for the demonstration of rubella-specific immunoglobulin M (IgM), and the results were compared with those of the density gradient centrifugation technique. In a retrospective study in which 157 sera were tested, the two techniques yielded identical results (55 IgM-positive and 102 IgM-negative samples). In a prospective study, 592 sere were examined; 8 IgM-positive results by SPRIST corresponded to a recent rubella infection or vaccination. Neither rheumatoid factor nor heterophil antibody seemed to interfere with the results of SPRIST. This test would be a useful and rapid routine technique for demonstration of the presence of virus-specific IgM in serum samples, particularly for viruses with a hemagglutinin. Except for anti-human IgM, no more reagents are needed than for widely used hemagglutination inhibition procedures.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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