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. 1981 Apr;13(4):801–804. doi: 10.1128/jcm.13.4.801-804.1981

Rapid bead counting and dispensing device for radioimmunoassays and enzyme-linked immunosorbent assays.

G W Hui
PMCID: PMC273884  PMID: 7014629

Abstract

A simple time- and labor-saving device is described for counting and dispensing beads used in solid-phase radioimmunoassay and enzyme-linked immunosorbent assay. In parallel radioimmunoassays, the data obtained with beads prepared by this device indicate an equivalent or superior sensitivity as compared with beads handled in the more tedious, conventional manner. The rapid handling of beads not only facilitates the processing of larger numbers of specimens, but also has the advantage of exposing up to 60 beads simultaneously to reactants in the same reaction tray.

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Selected References

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  1. Ball E. M. Solid phase radioimmunoassay for plant viruses. Virology. 1973 Oct;55(2):516–520. doi: 10.1016/0042-6822(73)90193-1. [DOI] [PubMed] [Google Scholar]
  2. Berson S. A., Yalow R. S. General principles of radioimmunoassay. Clin Chim Acta. 1968 Sep;22(1):51–69. doi: 10.1016/0009-8981(68)90247-7. [DOI] [PubMed] [Google Scholar]
  3. Daugharty H., Warfield D. T., Davis M. L. Solid-phase radioimmunoassay of total and influenza-specific immunoglobulin G. Appl Microbiol. 1972 Feb;23(2):360–367. doi: 10.1128/am.23.2.360-367.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Engvall E., Jonsson K., Perlmann P. Enzyme-linked immunosorbent assay. II. Quantitative assay of protein antigen, immunoglobulin G, by means of enzyme-labelled antigen and antibody-coated tubes. Biochim Biophys Acta. 1971 Dec 28;251(3):427–434. doi: 10.1016/0005-2795(71)90132-2. [DOI] [PubMed] [Google Scholar]
  5. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry. 1971 Sep;8(9):871–874. doi: 10.1016/0019-2791(71)90454-x. [DOI] [PubMed] [Google Scholar]
  6. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
  7. Farshy C. E., Klein G. C., Feeley J. C. Detection of antibodies to legionnaires disease organism by microagglutination and micro-enzyme-linked immunosorbent assay tests. J Clin Microbiol. 1978 Apr;7(4):327–331. doi: 10.1128/jcm.7.4.327-331.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hollinger F. B., Vorndam V., Dreesman G. R. Assay of Australia antigen and antibody employing double-antibody and solid-phase radioimmunoassay techniques and comparison with the passive hemagglutination methods. J Immunol. 1971 Oct;107(4):1099–1111. [PubMed] [Google Scholar]
  9. Irwin G. R., Allen A. M., Bancroft W. H., Willhight M., Russell P. K. Specificity and sensitivity of radioimmunoassay for hepatitis B antigen. Appl Microbiol. 1974 Oct;28(4):600–604. doi: 10.1128/am.28.4.600-604.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McLaren M. L., Mahgoub E. S., Georgakopoulos E. Preliminary investigation of the use of the enzyme linked immunosorbent assay (ELISA) in the serodiagnosis of mycetoma. Sabouraudia. 1978 Sep;16(3):225–228. doi: 10.1080/00362177885380301. [DOI] [PubMed] [Google Scholar]
  11. Salmon S. E., Mackey G., Fudenberg H. H. "Sandwich" solid phase radioimmunoassay for the quantitative deterimination of human immunoglobulins. J Immunol. 1969 Jul;103(1):129–137. [PubMed] [Google Scholar]
  12. Sanford B. A., Thomas V. L., Forland M., Carson S., Shelokov A. Immune response in urinary tract infection determined by radioimmunoassay and immunofluorescence: serum antibody levels against infecting bacterium and Enterobacteriaceae common antigen. J Clin Microbiol. 1978 Nov;8(5):575–579. doi: 10.1128/jcm.8.5.575-579.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Smith K. O., Gehle W. D. Magnetic transfer devices for use in solid-phase radioimmunoassays and enzyme-linked immunosorbent assays. J Infect Dis. 1977 Oct;136 (Suppl):S329–S336. doi: 10.1093/infdis/136.supplement_2.s329. [DOI] [PubMed] [Google Scholar]
  14. Stiffler-Rosenberg G., Fey H. Simple assay for staphylococcal enterotoxins A, B, and C: modification of enzyme-linked immunosorbent assay. J Clin Microbiol. 1978 Nov;8(5):473–479. doi: 10.1128/jcm.8.5.473-479.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Van Weemen B. K., Schuurs A. H.W.M. Immunoassay using antigen-enzyme conjugates. FEBS Lett. 1971 Jun 24;15(3):232–236. doi: 10.1016/0014-5793(71)80319-8. [DOI] [PubMed] [Google Scholar]
  16. Voller A., Bartlett A., Bidwell D. E., Clark M. F., Adams A. N. The detection of viruses by enzyme-linked immunosorbent assay (ELISA). J Gen Virol. 1976 Oct;33(1):165–167. doi: 10.1099/0022-1317-33-1-165. [DOI] [PubMed] [Google Scholar]
  17. Voller A., Bidwell D. E., Bartlett A. Enzyme immunoassays in diagnostic medicine. Theory and practice. Bull World Health Organ. 1976;53(1):55–65. [PMC free article] [PubMed] [Google Scholar]
  18. Voller A., Bidwell D. E. Enzyme-immunoassays for antibodies in measles, cytomegalovirus infections and after rubella vaccination. Br J Exp Pathol. 1976 Apr;57(2):243–247. [PMC free article] [PubMed] [Google Scholar]
  19. van Weemen B. K., Schuurs A. H. Immunoassay using hapten--enzyme conjugates. FEBS Lett. 1972 Jul 15;24(1):77–81. doi: 10.1016/0014-5793(72)80830-5. [DOI] [PubMed] [Google Scholar]

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