Abstract
The incorporation of hyperimmune serum into cell culture medium to control endogenous viral infections of primary cells can have a significant effect on the replication of other viruses. When commercial simian virus 5 or simian virus 40 antiserum was used with primary monkey kidney cell cultures, we found a significant inhibition (greater than 90%) of the replication of parainfluenza virus types 2 and 3 and reovirus type 1. In the viral diagnostic laboratory, the use of hyperimmune serum with primary monkey kidney cells may result in failure to isolate certain viruses if these cells are not first washed free of hyperimmune serum.
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Selected References
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- Barron A. L., Olshevsky C., Cohen M. M. Characteristics of the BGM line of cells from African green monkey kidney. Brief report. Arch Gesamte Virusforsch. 1970;32(4):389–392. doi: 10.1007/BF01250067. [DOI] [PubMed] [Google Scholar]
- Hughes J. H., Gnau J. M., Hilty M. D., Chema S., Ottolenghi A. C., Hamparian V. V. Picornaviruses: rapid differentiation and identification by immune electronmicroscopy and immunodiffusion. J Med Microbiol. 1977 May;10(2):203–212. doi: 10.1099/00222615-10-2-203. [DOI] [PubMed] [Google Scholar]
- Matsuno S., Inouye S., Kono R. Antigenic relationship between human and bovine rotaviruses as determined by neutralization, immune adherence hemagglutination, and complement fixation tests. Infect Immun. 1977 Sep;17(3):661–662. doi: 10.1128/iai.17.3.661-662.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Matsuno S., Inouye S., Kono R. Plaque assay of neonatal calf diarrhea virus and the neutralizing antibody in human sera. J Clin Microbiol. 1977 Jan;5(1):1–4. doi: 10.1128/jcm.5.1.1-4.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Petric M., Middleton P. J., Grant C., Tam J. S., Hewitt C. M. Lapine rotavirus: preliminary studies on epizoology and transmission. Can J Comp Med. 1978 Jan;42(1):143–147. [PMC free article] [PubMed] [Google Scholar]
- SWEET B. H., HILLEMAN M. R. The vacuolating virus, S.V. 40. Proc Soc Exp Biol Med. 1960 Nov;105:420–427. doi: 10.3181/00379727-105-26128. [DOI] [PubMed] [Google Scholar]