Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1981 May;13(5):895–898. doi: 10.1128/jcm.13.5.895-898.1981

Laboratory evaluation of the AutoMicrobic system for identification of Enterobacteriaceae.

J W Freeman, R W Rowland, S B Overman, N L Goodman
PMCID: PMC273913  PMID: 7016913

Abstract

The Enterobacteriaceae Biochemical Card used with the AutoMicrobic system (Vitek Systems, Inc., Hazelwood, Mo.) was compared with the API 20E system (Analytab Products, Plainview, N.Y.) for identifying members of the family Enterobacteriaceae. A total of 1,401 clinical isolates representing 18 species were identified by the AutoMicrobic and API 20E systems over a 5-month period. Discrepancies between these systems were resolved by conventional methods. Overall, 98.3% of the isolates were identified correctly by the AutoMicrobic system in 8 h, with 94.2% having an AutoMicrobic system confidence level greater than or equal to 90%; 92.9% of the isolates were identified correctly by the 20E system after 24 h of incubation. Discrepancies between the two systems occurred in 3.1% of the isolates. Of these, 40.9 and 59.1% proved to be correct by the AutoMicrobic and API 20E systems, respectively.

Full text

PDF
895

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aldridge C., Jones P. W., Gibson S., Lanham J., Meyer M., Vannest R., Charles R. Automated microbiological detection/identification system. J Clin Microbiol. 1977 Oct;6(4):406–413. doi: 10.1128/jcm.6.4.406-413.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Isenberg H. D., Gavan T. L., Smith P. B., Sonnenwirth A., Taylor W., Martin W. J., Rhoden D., Balows A. Collaborative investigation of the AutoMicrobic System Enterobacteriaceae biochemical card. J Clin Microbiol. 1980 Jun;11(6):694–702. doi: 10.1128/jcm.11.6.694-702.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Isenberg H. D., Gavan T. L., Sonnenwirth A., Taylor W. I., Washington J. A., 2nd Clinical laboratory evaluation of automated microbial detection/identification system in analysis of clinical urine specimens. J Clin Microbiol. 1979 Aug;10(2):226–230. doi: 10.1128/jcm.10.2.226-230.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Smith P. B., Gavan T. L., Isenberg H. D., Sonnenwirth A., Taylor W. I., Washington J. A., 2nd, Balows A. Multi-laboratory evaluation of an automated microbial detection/identification system. J Clin Microbiol. 1978 Dec;8(6):657–666. doi: 10.1128/jcm.8.6.657-666.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Smith P. B., Tomfohrde K. M., Rhoden D. L., Balows A. API system: a multitube micromethod for identification of Enterobacteriaceae. Appl Microbiol. 1972 Sep;24(3):449–452. doi: 10.1128/am.24.3.449-452.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES