Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1981 May;13(5):969–975. doi: 10.1128/jcm.13.5.969-975.1981

Improved technique for isolation of Mycobacterium kansasii from water.

B L Powell Jr, J E Steadham
PMCID: PMC273925  PMID: 7016916

Abstract

A new concentration procedure, together with a new isolation medium, resulted in a 10- to 100-fold increase in the possibility of identifying Mycobacterium kansasii from water samples in comparison to a previously used procedure. In a survey which included both potable and natural water samples from many sites within the state of Texas, nine isolations of high-catalase strains of M. kansasii were obtained from 232 water samples tested. Acid-fast smear results were compared with mycobacterial isolations. An isolate from a river in central Texas is the first high-catalase strain of M. kansasii encountered in a natural water supply. Surveys of water samples from two Texas towns indicate that chlorine levels may influence the number of recoverable mycobacteria in water supplies.

Full text

PDF
969

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bailey R. K., Wyles S., Dingley M., Hesse F., Kent G. W. The isolation of high catalase Mycobacterium kansasii from tap water. Am Rev Respir Dis. 1970 Mar;101(3):430–431. doi: 10.1164/arrd.1970.101.3.430. [DOI] [PubMed] [Google Scholar]
  2. Engel H. W., Berwald L. G., Havelaar A. H. The occurrence of Mycobacterium kansasii in tapwater. Tubercle. 1980 Mar;61(1):21–26. doi: 10.1016/0041-3879(80)90055-0. [DOI] [PubMed] [Google Scholar]
  3. Goslee S., Wolinsky E. Water as a source of potentially pathogenic mycobacteria. Am Rev Respir Dis. 1976 Mar;113(3):287–292. doi: 10.1164/arrd.1976.113.3.287. [DOI] [PubMed] [Google Scholar]
  4. Gruft H., Loder A., Osterhout M., Parker B. D., Falkinham J. O., 3rd Postulated sources of Mycobacterium intracellulare and Mycobacterium scrofulaceum infection: isolation of mycobacteria from estuaries and ocean waters. Am Rev Respir Dis. 1979 Dec;120(6):1385–1388. doi: 10.1164/arrd.1979.120.6.1385. [DOI] [PubMed] [Google Scholar]
  5. KUBICA G. P., BEAM R. E., PALMER J. W. A METHOD FOR THE ISOLATION OF UNCLASSIFIED ACID-FAST BACILLI FROM SOIL AND WATER. Am Rev Respir Dis. 1963 Nov;88:718–720. doi: 10.1164/arrd.1963.88.5.718. [DOI] [PubMed] [Google Scholar]
  6. Kubica G. P., Kim T. H. Preservation of mycobacteria at -70 degrees C: survival of unfrozen suspensions in transit. Tubercle. 1979 Mar;60(1):37–43. doi: 10.1016/0041-3879(79)90054-0. [DOI] [PubMed] [Google Scholar]
  7. MIDDLEBROOK G., COHN M. L. Bacteriology of tuberculosis: laboratory methods. Am J Public Health Nations Health. 1958 Jul;48(7):844–853. doi: 10.2105/ajph.48.7.844. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. McSwiggan D. A., Collins C. H. The isolation of M. kansasii and M. xenopi from water systems. Tubercle. 1974 Dec;55(4):291–297. doi: 10.1016/0041-3879(74)90038-5. [DOI] [PubMed] [Google Scholar]
  9. Medek B., Kubín M., Hudec V., Chobot S., Olsovsky Z., Pelikán M., Richtrová S., Svandovă E., Malis J. Endemický výskyt onemocnení vyvolaných mycobacterium kansasii v karvinské průmyslové aglomeraci. Cas Lek Cesk. 1979 Mar 9;118(10):307–314. [PubMed] [Google Scholar]
  10. Steadham J. E. High-catalase strains of Mycobacterium kansasii isolated from water in Texas. J Clin Microbiol. 1980 May;11(5):496–498. doi: 10.1128/jcm.11.5.496-498.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Tison F., Tacquet A., Devulder B. Recherche des mycobactéries dans les eaux de piscines et les eaux usées de la région du nord. Techniques. Résultats. Ann Inst Pasteur Lille. 1967;18:167–176. [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES