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. 1981 May;13(5):982–988. doi: 10.1128/jcm.13.5.982-988.1981

Frequency of preclumped virus in routine fecal specimens from patients with acute nonbacterial gastroenteritis.

H K Narang, A A Codd
PMCID: PMC273927  PMID: 6263948

Abstract

A low-speed centrifugation technique for the preparation of grids after minimal purification of fecal extracts is described for examination of viruses by direct electron microscopy using negative staining. Results showed that adenovirus, astrovirus, rotavirus, and "small round" viruses were frequently shed into the gastrointestinal tract in clumps of variable size. Differential centrifugation study showed that a substantial proportion of the virus in the sample was lost in the initial pellet at the first step of clarification; this finding casts doubt on the validity of immune electron microscopy for direct typing of strains of these viruses from stools. In addition, particle counts based on conventional specimen processing are likely to grossly underestimate the true value.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Banatvala J. E., Totterdell B., Chrystie I. L., Woode G. N. In-vitro detection of human rotaviruses. Lancet. 1975 Oct 25;2(7939):821–821. doi: 10.1016/s0140-6736(75)80057-2. [DOI] [PubMed] [Google Scholar]
  2. Bishop R. F., Davidson G. P., Holmes I. H., Ruck B. J. Detection of a new virus by electron microscopy of faecal extracts from children with acute gastroenteritis. Lancet. 1974 Feb 2;1(7849):149–151. doi: 10.1016/s0140-6736(74)92440-4. [DOI] [PubMed] [Google Scholar]
  3. Bishop R. F., Davidson G. P., Holmes I. H., Ruck B. J. Virus particles in epithelial cells of duodenal mucosa from children with acute non-bacterial gastroenteritis. Lancet. 1973 Dec 8;2(7841):1281–1283. doi: 10.1016/s0140-6736(73)92867-5. [DOI] [PubMed] [Google Scholar]
  4. Davidson G. P., Bishop R. F., Townley R. R., Holmes I. H. Importance of a new virus in acute sporadic enteritis in children. Lancet. 1975 Feb 1;1(7901):242–246. doi: 10.1016/s0140-6736(75)91140-x. [DOI] [PubMed] [Google Scholar]
  5. Flewett T. H., Bryden A. S., Davies H. Letter: Virus particles in gastroenteritis. Lancet. 1973 Dec 29;2(7844):1497–1497. doi: 10.1016/s0140-6736(73)92760-8. [DOI] [PubMed] [Google Scholar]
  6. Flewett T. H., Bryden A. S., Davies H., Woode G. N., Bridger J. C., Derrick J. M. Relation between viruses from acute gastroenteritis of children and newborn calves. Lancet. 1974 Jul 13;2(7872):61–63. doi: 10.1016/s0140-6736(74)91631-6. [DOI] [PubMed] [Google Scholar]
  7. Gary G. W., Jr, Hierholzer J. C., Black R. E. Characteristics of noncultivable adenoviruses associated with diarrhea in infants: a new subgroup of human adenoviruses. J Clin Microbiol. 1979 Jul;10(1):96–103. doi: 10.1128/jcm.10.1.96-103.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HUMMELER K., ANDERSON T. F., BROWN R. A. Identification of poliovirus particles of different antigenicity by specific agglutination as seen in the electron microscope. Virology. 1962 Jan;16:84–90. doi: 10.1016/0042-6822(62)90205-2. [DOI] [PubMed] [Google Scholar]
  9. Holmes I. H., Mathan M., Bhat P., Albert M. J., Swaminathan S. P., Maiya P. P., Pereira S. M., Baker S. J. Letter: Orbiviruses and gastroenteritis. Lancet. 1974 Sep 14;2(7881):658–659. doi: 10.1016/s0140-6736(74)91987-4. [DOI] [PubMed] [Google Scholar]
  10. Kapikian A. Z., Wyatt R. G., Dolin R., Thornhill T. S., Kalica A. R., Chanock R. M. Visualization by immune electron microscopy of a 27-nm particle associated with acute infectious nonbacterial gastroenteritis. J Virol. 1972 Nov;10(5):1075–1081. doi: 10.1128/jvi.10.5.1075-1081.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Madeley C. R., Cosgrove B. P., Bell E. J., Fallon R. J. Stool viruses in babies in Glasgow. I. Hospital admissions with diarrhoea. J Hyg (Lond) 1977 Apr;78(2):261–273. doi: 10.1017/s0022172400056151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Madeley C. R., Cosgrove B. P., Bell E. J. Stool viruses in babies in Glasgow 2. Investigation of normal newborns in hospital. J Hyg (Lond) 1978 Oct;81(2):285–294. doi: 10.1017/s0022172400025122. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Matsuno S., Inouye S., Kono R. Plaque assay of neonatal calf diarrhea virus and the neutralizing antibody in human sera. J Clin Microbiol. 1977 Jan;5(1):1–4. doi: 10.1128/jcm.5.1.1-4.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Morishima T., Nagayoshi S., Ozaki T., Isomura S., Suzuki S. Letter: Immunofluorescence of human reovirus-like agent of infantile diarrhoea. Lancet. 1976 Sep 25;2(7987):695–696. doi: 10.1016/s0140-6736(76)92517-4. [DOI] [PubMed] [Google Scholar]
  15. Narang H. K., Codd A. A. A low-speed centrifugation technique for the preparation of grids for direct virus examination by electron microscopy. J Clin Pathol. 1979 Mar;32(3):304–305. doi: 10.1136/jcp.32.3.304. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Narang H. K., Codd A. A. Enterovirus typing by immune electron microscopy using low-speed centrifugation. J Clin Pathol. 1980 Feb;33(2):191–194. doi: 10.1136/jcp.33.2.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Narang H. K., Codd A. A. Preponderance of rotavirus in clumped form in patients with acute gastroenteritis. Lancet. 1980 May 31;1(8179):1192–1193. doi: 10.1016/s0140-6736(80)91650-5. [DOI] [PubMed] [Google Scholar]
  18. Orstavik I., Figenschau K. J., Ulstrup J. C. Letter: "Rotavirus" in stored specimens of faecal extracts. Lancet. 1974 Nov 2;2(7888):1083–1083. doi: 10.1016/s0140-6736(74)92187-4. [DOI] [PubMed] [Google Scholar]
  19. Paver W. K., Ashley C. R., Caul E. O., Clarke S. K. A small virus in human faeces. Lancet. 1973 Feb 3;1(7797):237–240. doi: 10.1016/s0140-6736(73)90072-x. [DOI] [PubMed] [Google Scholar]
  20. Purdham D. R., Purdham P. A., Evans N., McNeish A. S. Letter: Isolation of human rotavirus using human embryonic gut monolayers. Lancet. 1975 Nov 15;2(7942):977–977. doi: 10.1016/s0140-6736(75)90386-4. [DOI] [PubMed] [Google Scholar]
  21. Smith E. M., Estes M. K., Graham D. Y., Gerba C. P. A plaque assay for the simian rotavirus SAII. J Gen Virol. 1979 Jun;43(3):513–519. doi: 10.1099/0022-1317-43-3-513. [DOI] [PubMed] [Google Scholar]
  22. Wyatt R. G., Gill V. W., Sereno M. M., Kalica A. R., VanKirk D. H., Chanock R. M., Kapikian A. Z. Letter: Probable in-vitro cultivation of human reovirus-like agent of infantile diarroea. Lancet. 1976 Jan 10;1(7950):98–99. doi: 10.1016/s0140-6736(76)90202-6. [DOI] [PubMed] [Google Scholar]
  23. Zissis G., Lambert J. P., De Kegel D. Routine diagnosis of human rotaviruses in stools. J Clin Pathol. 1978 Feb;31(2):175–178. doi: 10.1136/jcp.31.2.175. [DOI] [PMC free article] [PubMed] [Google Scholar]

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