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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1981 May;13(5):996–997. doi: 10.1128/jcm.13.5.996-997.1981

Improved method for trypsin digestion of Paraplast sections before immunofluorescence staining.

D Rowse-Eagle, H D Watson, G H Tignor
PMCID: PMC273931  PMID: 7016918

Abstract

Formalin-fixed, Paraplast-embedded tissue sections mounted on subbed slides were digested with crude trypsin in modified Sorensen phosphate buffer containing sodium fluoride before immunofluorescence staining. This method prevented detachment of sections from slides during processing, thereby permitting immunofluorescence and histological examinations of adjacent serial sections.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Johnson K. P., Swoveland P. T., Emmons R. W. Diagnosis of rabies by immunofluorescence in trypsin-treated histologic sections. JAMA. 1980 Jul 4;244(1):41–43. [PubMed] [Google Scholar]
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