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. 1981 Jun;13(6):1011–1016. doi: 10.1128/jcm.13.6.1011-1016.1981

Clinical manifestations of diarrhea in calves infected with rotavirus and enterotoxigenic Escherichia coli.

S R Tzipori, T J Makin, M L Smith, F L Krautil
PMCID: PMC273940  PMID: 6265493

Abstract

The susceptibility of gnotobiotic, colostrum-derived, or suckling calves to four bovine rotavirus isolates was found to be age dependent. Calves older than 7 days remained clinically normal, although they excreted virus in their feces and subsequently developed antibody against the virus, Enterotoxigenic Escherichia coli, fed to gnotobiotic, colostrum-deprived, or suckling calves ranging in age from a few hours to 26 days old, only caused diarrhea in animals younger than 24 h old. In contrast, diarrhea was consistently induced in 1- and 2-week-old calves infected with both enterotoxigenic E. coli and rotavirus. In general, diarrhea appeared after a rotavirus incubation period of approximately 3 days and was independent of the order in which the two microbial agents were given, the age of the calf, or the level of circulating rotavirus antibodies. The disease episode coincided with the excretion of rotavirus, rather than enterotoxigenic E. coli, in the feces. Infection with enterotoxigenic E. coli became established within 24 h of inoculation, and in older calves enterotoxigenic E. coli was often excreted in very small numbers and for a longer period than rotavirus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Acres S. D., Babiuk L. A. Studies on rotaviral antibody in bovine serum and lacteal secretions, using radioimmunoassay. J Am Vet Med Assoc. 1978 Sep 1;173(5 Pt 2):555–559. [PubMed] [Google Scholar]
  2. Acres S. D., Isaacson R. E., Babiuk L. A., Kapitany R. A. Immunization of calves against enterotoxigenic colibacillosis by vaccinating dams with purified K99 antigen and whole cell bacterins. Infect Immun. 1979 Jul;25(1):121–126. doi: 10.1128/iai.25.1.121-126.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Acres S. D., Radostits O. M. The efficacy of a modified live reo-like virus vaccine and an E. coli bacterin for prevention of acute undifferentiated neonatal diarrhea of beef calves. Can Vet J. 1976 Aug;17(8):197–212. [PMC free article] [PubMed] [Google Scholar]
  4. Acres S. D., Saunders J. R., Radostits O. M. Acute undifferentiated neonatal diarrhea of beef calves: the prevalence of enterotoxigenic E. coli, reo-like (rota) virus and other enteropathogens in cow-calf herds. Can Vet J. 1977 May;18(5):113–121. [PMC free article] [PubMed] [Google Scholar]
  5. Dean A. G., Ching Y. C., Williams R. G., Harden L. B. Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu. J Infect Dis. 1972 Apr;125(4):407–411. doi: 10.1093/infdis/125.4.407. [DOI] [PubMed] [Google Scholar]
  6. Gouet P., Contrepois M., Dubourguier H. C., Riou Y., Scherrer R., Laporte J., Vautherot J. F., Cohen J., L'Haridon R. The experimental production of diarrhoea in colostrum deprived axenic and gnotoxenic calves with enteropathogenic Escherichia coli, rotavirus, coronavirus and in a combined infection of rotavirus and E. coli. Ann Rech Vet. 1978;9(3):433–440. [PubMed] [Google Scholar]
  7. Logan E. F., Pearson G. R., McNulty M. S. Quantitative observations on experimental reo-like virus (rotavirus) infection in colostrum-deprived calves. Vet Rec. 1979 Mar 10;104(10):206–209. doi: 10.1136/vr.104.10.206. [DOI] [PubMed] [Google Scholar]
  8. Logan E. F., Pearson G. R., McNulty M. S. Studies on the immunity of the calf to colibacillosis--VII: the experimental reproduction of enteric colibacillosis in colostrum-fed calves. Vet Rec. 1977 Nov 26;101(22):443–446. doi: 10.1136/vr.101.22.443. [DOI] [PubMed] [Google Scholar]
  9. McNulty M. S., McFerran J. B., Bryson D. G., Logan E. F., Curran W. L. Studies on rotavirus infection and diarrhoea in young calves. Vet Rec. 1976 Sep 18;99(12):229–230. doi: 10.1136/vr.99.12.229. [DOI] [PubMed] [Google Scholar]
  10. McNulty M. S. Rotaviruses. J Gen Virol. 1978 Jul;40(1):1–18. doi: 10.1099/0022-1317-40-1-1. [DOI] [PubMed] [Google Scholar]
  11. Mebus C. A., Underdahl N. R., Rhodes M. B., Twiehaus M. J. Further studies on neonatal calf diarrhea virus. Proc Annu Meet U S Anim Health Assoc. 1969;73:97–99. [PubMed] [Google Scholar]
  12. Moon H. W., Whipp S. C., Skartvedt S. M. Etiologic diagnosis of diarrheal disease of calves: frequency and methods for detecting enterotoxin and K99 antigen production by Escherichia cola. Am J Vet Res. 1976 Sep;37(9):1025–1029. [PubMed] [Google Scholar]
  13. Pearson G. R., Logan E. F., Brennan G. P. Scanning electron microscopy of the small intestine of a normal unsuckled calf and a calf with enteric colibacillosis. Vet Pathol. 1978 May;15(3):400–406. doi: 10.1177/030098587801500316. [DOI] [PubMed] [Google Scholar]
  14. Pearson G. R., McNulty M. S., Logan E. F. Pathological changes in the small intestine of neonatal calves with enteric colibacillosis. Vet Pathol. 1978 Jan;15(1):92–101. doi: 10.1177/030098587801500111. [DOI] [PubMed] [Google Scholar]
  15. Sack D. A., Sack R. B. Test for enterotoxigenic Escherichia coli using Y-1 adrenal cells in miniculture. Infect Immun. 1975 Feb;11(2):334–336. doi: 10.1128/iai.11.2.334-336.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schlafer D. H., Scott F. W. Prevalence of neutralizing antibody to the calf rotavirus in New York cattle. Cornell Vet. 1979 Jul;69(3):262–271. [PubMed] [Google Scholar]
  17. Smith H. W., Halls S. Observations by the ligated intestinal segment and oral inoculation methods on Escherichia coli infections in pigs, calves, lambs and rabbits. J Pathol Bacteriol. 1967 Apr;93(2):499–529. doi: 10.1002/path.1700930211. [DOI] [PubMed] [Google Scholar]
  18. Tzipori S. R., Makin T. J., Smith M. L. The clinical response of gnotobiotic calves, pigs and lambs to inoculation with human, calf, pig and foal rotavirus isolates. Aust J Exp Biol Med Sci. 1980 Jun;58(3):309–318. doi: 10.1038/icb.1980.31. [DOI] [PubMed] [Google Scholar]
  19. Woode G. N., Bew M. E., Dennis M. J. Studies on cross protection induced in calves by rotaviruses of calves, children and foals. Vet Rec. 1978 Jul 8;103(2):32–34. doi: 10.1136/vr.103.2.32. [DOI] [PubMed] [Google Scholar]
  20. Woode G. N., Bridger J. C., Hall G., Dennis M. J. The isolation of a reovirus-like agent associated with diarrhoea in colostrum-deprived calves in Great Britain. Res Vet Sci. 1974 Jan;16(1):102–105. [PubMed] [Google Scholar]

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