Abstract
The results of single radial immunodiffusion assays of influenza virus hemagglutinin were found to be greatly altered by small antigenic differences between test and reference strains. When such differences were present, the precise specificity of the antiserum used had a critical effect on the measured hemagglutinin antigen content obtained.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brand C. M., Skehel J. J. Crystalline antigen from the influenza virus envelope. Nat New Biol. 1972 Aug 2;238(83):145–147. doi: 10.1038/newbio238145a0. [DOI] [PubMed] [Google Scholar]
- Gandhi S. S. The effect of formaldehyde treatment of influenza virus on the assay of its haemagglutinin antigen content by the single-radial-immunodiffusion (SRD) technique. J Biol Stand. 1978;6(2):121–126. doi: 10.1016/s0092-1157(78)80043-2. [DOI] [PubMed] [Google Scholar]
- Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
- Nash D. R., Scolari L., Heremans J. F. Further studies on single radial immunodiffusion. IV. Quantitation of heterologous antigens by means of cross-reacting antisera. Immunochemistry. 1970 Mar;7(3):265–274. [PubMed] [Google Scholar]
- Reichert E., Majer M., Mauler R. Results of the single-radial-diffusion test with formaldehyde-treated influenza virus. Dev Biol Stand. 1977 Jun 1;39:187–191. [PubMed] [Google Scholar]
- Russ G., Styk B., Poláková K. Antigenic glycopolypeptides HA1 and HA2 of influenza virus haemagglutinin. II. Reactivity with rabbit sera against intact virus and purified undissociated haemagglutinin. Acta Virol. 1978 Sep;22(5):371–382. [PubMed] [Google Scholar]
- Webster R. G., Kendal A. P., Gerhard W. Analysis of antigenic drift in recently isolated influenza A (H1N1) viruses using monoclonal antibody preparations. Virology. 1979 Jul 15;96(1):258–264. doi: 10.1016/0042-6822(79)90189-2. [DOI] [PubMed] [Google Scholar]
- Webster R. G. The immune response to influenza virus. 3. Changes in the avidity and specificity of early IgM and IgG antibodies. Immunology. 1968 Jan;14(1):39–52. [PMC free article] [PubMed] [Google Scholar]
- Wood J. M., Newman R. W., Schild G. C., Dunleavy U. Reagents for single-radial-diffusion assay of the haemagglutinin antigen of influenza virus A/USSR/92/77 (H1N1). J Biol Stand. 1979 Apr;7(2):147–150. doi: 10.1016/s0092-1157(79)80046-3. [DOI] [PubMed] [Google Scholar]
- Wood J. M., Schild G. C., Newman R. W., Seagroatt V. An improved single-radial-immunodiffusion technique for the assay of influenza haemagglutinin antigen: application for potency determinations of inactivated whole virus and subunit vaccines. J Biol Stand. 1977;5(3):237–247. doi: 10.1016/s0092-1157(77)80008-5. [DOI] [PubMed] [Google Scholar]