Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1981 Nov;14(5):530–533. doi: 10.1128/jcm.14.5.530-533.1981

Extraction of group A streptococcal M protein with nitrous acid.

K Hafez, A M El Kholy, R R Facklam
PMCID: PMC273982  PMID: 7309850

Abstract

Specific precipitating and opsonic neutralizing antigens were identified in dilute-nitrous acid extracts of group A streptococci. This was done by decreasing the temperature and time of exposure of the streptococcal cells to the nitrous acid. Ninety percent of 31 strains of group A streptococcal dilute-nitrous acid extracts formed precipitin lines of identity with conventional Lancefield extracts of the same group A streptococci.

Full text

PDF
530

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beachey E. H., Campbell G. L., Ofek I. Peptic digestion of streptococcal M protein. II. Extraction of M antigen from group A streptococci with pepsin. Infect Immun. 1974 May;9(5):891–896. doi: 10.1128/iai.9.5.891-896.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cunningham M., Beachey E. H. Immunochemical properties of streptococcal M protein purified by isoelectric focusing. J Immunol. 1975 Oct;115(4):1002–1006. [PubMed] [Google Scholar]
  3. El Kholy A. M., Hafez K., Guirguis N., Facklam R. R., Johnson D. R., Wannamaker L. Heterogeneity of group A type-specific antibodies. Infect Immun. 1980 Mar;27(3):953–959. doi: 10.1128/iai.27.3.953-959.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fischetti V. A., Gotschlich E. C., Siviglia G., Zabriskie J. B. Streptococcal M protein extracted by nonionic detergent. I. Properties of the antiphagocytic and type-specific molecules. J Exp Med. 1976 Jul 1;144(1):32–53. doi: 10.1084/jem.144.1.32. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fischetti V. A. Streptococcal M protein extracted by nonionic detergent. II. Analysis of the antibody response to the multiple antigenic determinants of the M-protein molecule. J Exp Med. 1977 Oct 1;146(4):1108–1123. doi: 10.1084/jem.146.4.1108. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fox E. N., Wittner M. K. New observations on the structure and antigenicity of the M proteins of the group A streptococcus. Immunochemistry. 1969 Jan;6(1):11–24. doi: 10.1016/0019-2791(69)90174-8. [DOI] [PubMed] [Google Scholar]
  7. Havlícek J. M protein of type 12 Strepto coccus pyogenes. Isolation by electrofocusing and some molecular weight-dependent properties. Pathol Microbiol (Basel) 1975;42(3):147–158. [PubMed] [Google Scholar]
  8. KRAUSE R. M. Studies on the bacteriophages of hemolytic streptococci. II. Antigens released from the streptococcal cell wall by a phage-associated lysin. J Exp Med. 1958 Dec 1;108(6):803–821. doi: 10.1084/jem.108.6.803. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MAXTED W. R. The active agent in nascent phage lysis of streptococci. J Gen Microbiol. 1957 Jun;16(3):584–595. doi: 10.1099/00221287-16-3-584. [DOI] [PubMed] [Google Scholar]
  10. Rotta J., Krause R. M., Lancefield R. C., Everly W., Lackland H. New approaches for the laboratory recognition of M types of group A streptococci. J Exp Med. 1971 Nov 1;134(5):1298–1315. doi: 10.1084/jem.134.5.1298. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Russell H., Facklam R. R. Guanidine extraction of streptococcal M protein. Infect Immun. 1975 Sep;12(3):679–686. doi: 10.1128/iai.12.3.679-686.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. SALTON M. R. J., HORNE R. W. Studies of the bacterial cell wall. II. Methods of preparation and some properties of cell walls. Biochim Biophys Acta. 1951 Jul;7(2):177–197. doi: 10.1016/0006-3002(51)90017-0. [DOI] [PubMed] [Google Scholar]
  13. SCHMIDT W. C. THE LYSIS OF CELL WALLS OF GROUP A STREPTOCOCCI BY STREPTOMYCES ALBUS ENZYME TREATED WITH DIISOPROPYL FLUOROPHOSPHATE. CHARACTERISTICS OF THE LYTIC REACTION AND THE SOLUBLE CELL WALL FRAGMENTS. J Exp Med. 1965 May 1;121:771–792. doi: 10.1084/jem.121.5.771. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. SLADE H. D., VETTER J. K. Studies on Streptococcus pyogenes. I. Observations on the microscopical and biological aspects of the disintegration and solubilization of a type 6 strain by sonic oscillation. J Bacteriol. 1956 Feb;71(2):236–243. doi: 10.1128/jb.71.2.236-243.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Swanson J., Hsu K. C., Gotschlich E. C. Electron microscopic studies on streptococci. I. M antigen. J Exp Med. 1969 Nov 1;130(5):1063–1091. doi: 10.1084/jem.130.5.1063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Vosti K. L., Williams W. K. Extraction of streptococcal type 12 M protein by cyanogen bromide. Infect Immun. 1978 Aug;21(2):546–555. doi: 10.1128/iai.21.2.546-555.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Widdowson J. P., Maxted W. R., Pinney A. M. An M-associated protein antigen (MAP) of group A streptococci. J Hyg (Lond) 1971 Dec;69(4):553–564. doi: 10.1017/s0022172400021823. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. el Kholy A., Wannamaker L. W., Krause R. M. Simplified extraction procedure for serological grouping of beta-hemolytic streptococci. Appl Microbiol. 1974 Nov;28(5):836–839. doi: 10.1128/am.28.5.836-839.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES