Table 1.
Electronic absorption properties for Tt H-NOX, Hb, and Mb in the reduced and CO, NO, and O2-bound forms.a
| Protein | Ligand | Soret | α/β | Ref. |
|---|---|---|---|---|
| Tt WT | reduced | 430 | 563 | (19) |
| Tt P115A | 432 | 563 | this work | |
| Tt I5L | 431 | 569 | this work | |
| Tt P115A/I5L | 431 | 566 | this work | |
| Hb | 430 | 555 | (30) | |
| Mb | 434 | 556 | (30) | |
| Tt WT | CO | 423 | 567/541 | (19) |
| Tt P115A | 424 | 568/538 | this work | |
| Tt I5L | 424 | 565/541 | this work | |
| Tt P115A/I5L | 424 | 570/542 | this work | |
| Hb | 419 | 569/540 | (30) | |
| Mb | 423 | 579/542 | (30) | |
| Tt WT | NO | 420 | 575/547 | (19) |
| Tt P115A | 418 | 573/543 | this work | |
| Tt I5L | 421 | 572/539 | this work | |
| Tt P115A/I5L | 421 | 568/535 | this work | |
| Hb | 418 | 575/545 | (30) | |
| Mb | 421 | 575/543 | (30) | |
| Tt WT | O2 | 416 | 591/556 | (19) |
| Tt P115A | 416 | 584/548 | this work | |
| Tt I5L | 418 | 587/550 | this work | |
| Tt P115A/I5L | 417 | 588/552 | this work | |
| Hb | 415 | 576/541 | (30) | |
| Mb | 418 | 580/542 | (30) | |
a
All peak positions are reported in nm.