Abstract
Biochemically atypical strains classified as Vibrio cholerae were characterized by biochemical reactions, serology, antibiotic susceptibility testing, and deoxyribonucleic acid relatedness. Strains with the following atypical reactions were shown to be V. cholerae: mannose negative, mannitol negative, lysine decarboxylase negative, no growth in the presence of 5% NaCl, salicin and cellobiose positive. Sucrose-negative strains were shown to constitute a new species, Vibrio mimicus, whose type strain is 1721-77 (ATCC 33653). In addition to its negative sucrose reaction, V mimicus was differentiated from V. cholerae by its negative Voges-Proskauer, corn oil, and Jordan tartrate reactions and by its sensitivity to polymyxin. V. mimicus was isolated from shellfish and water, as well as from human diarrheal stools and ear infections. Most strains were typable with antisera against V. cholerae. Strains from three serogroups produced either a heat-labile or a heat-stable enterotoxin.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bauer A. W., Kirby W. M., Sherris J. C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966 Apr;45(4):493–496. [PubMed] [Google Scholar]
- Brenner D. J., Fanning G. R., Johnson K. E., Citarella R. V., Falkow S. Polynucleotide sequence relationships among members of Enterobacteriaceae. J Bacteriol. 1969 May;98(2):637–650. doi: 10.1128/jb.98.2.637-650.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Citarella R. V., Colwell R. R. Polyphasic taxonomy of the genus Vibrio: polynucleotide sequence relationships among selected Vibrio species. J Bacteriol. 1970 Oct;104(1):434–442. doi: 10.1128/jb.104.1.434-442.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dean A. G., Ching Y. C., Williams R. G., Harden L. B. Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu. J Infect Dis. 1972 Apr;125(4):407–411. doi: 10.1093/infdis/125.4.407. [DOI] [PubMed] [Google Scholar]
- Donta S. T., King M., Sloper K. Induction of steroidogenesis in tissue culture by cholera enterotoxin. Nat New Biol. 1973 Jun 20;243(129):246–247. doi: 10.1038/newbio243246a0. [DOI] [PubMed] [Google Scholar]
- Hickman F. W., Farmer J. J., 3rd Salmonella typhi: identification, antibiograms, serology, and bacteriophage typing. Am J Med Technol. 1978 Dec;44(12):1149–1159. [PubMed] [Google Scholar]
- Hickman F. W., Framer J. J., 3rd, Steigerwalt A. G., Brenner D. J. Unusual groups of Morganella ("Proteus") morganii isolated from clinical specimens: lysine-positive and ornithine-negative biogroups. J Clin Microbiol. 1980 Jul;12(1):88–94. doi: 10.1128/jcm.12.1.88-94.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SHEWAN J. M., HODGKISS W. A method for the rapid differentiation of certain nonpathogenic, asporogenous bacilli. Nature. 1954 Jan 30;173(4396):208–209. doi: 10.1038/173208b0. [DOI] [PubMed] [Google Scholar]
- Sakazaki R., Shimada T. Serovars of Vibrio cholerae identified during 1970-1975. Jpn J Med Sci Biol. 1977 Oct;30(5):279–282. doi: 10.7883/yoken1952.30.279. [DOI] [PubMed] [Google Scholar]
- Smith H. L., Jr Serotyping of non-cholera vibrios. J Clin Microbiol. 1979 Jul;10(1):85–90. doi: 10.1128/jcm.10.1.85-90.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yolken R. H., Greenberg H. B., Merson M. H., Sack R. B., Kapikian A. Z. Enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin. J Clin Microbiol. 1977 Nov;6(5):439–444. doi: 10.1128/jcm.6.5.439-444.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]