Abstract
Pluronic polyols markedly improved the growth of two human lymphocyte cell lines when added to the growth medium in concentrations of 0.05 to 0.1%. The results of the current studies suggest that, in addition to the protective effect of polyols against mechanical damage of mammalian cells in submerged cultures, the pluronic compounds may also, by lowering surface tension, facilitate transport of metabolites into cells and thus increase the growth rate.
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Selected References
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- Granger G. A., Moore G. E., White J. G., Matzinger P., Sundsmo J. S., Shupe S., Kolb W. P., Kramer J., Glade P. R. Production of lymphotoxin and migration inhibitory factor by established human lymphocytic cell lines. J Immunol. 1970 Jun;104(6):1476–1485. [PubMed] [Google Scholar]
- Mizrahi A., Miller G. Role of glycols and tweens in the production of ergot alkaloids by Claviceps paspali. J Bacteriol. 1969 Mar;97(3):1155–1159. doi: 10.1128/jb.97.3.1155-1159.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mizrahi A., Moore G. E. Partial substitution of serum in hematopoietic cell line media by synthetic polymers. Appl Microbiol. 1970 Jun;19(6):906–910. doi: 10.1128/am.19.6.906-910.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moore G. E., Gerner R. E. Cancer immunity--hypothesis and clinical trial of lymphocytotherapy for malignant diseases. Ann Surg. 1970 Oct;172(4):733–739. doi: 10.1097/00000658-197010000-00017. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moore G. E., Gerner R. E., Franklin H. A. Culture of normal human leukocytes. JAMA. 1967 Feb 20;199(8):519–524. [PubMed] [Google Scholar]
- Najarian J. S., Simmons R. L., Gewurz H., Moberg A., Merkel F., Moore G. E. Anti-serum to cultured human lymphoblasts: preparation, purification and immunosuppressive properties in man. Ann Surg. 1969 Oct;170(4):617–632. doi: 10.1097/00000658-196910000-00013. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RUNYAN W. S., GEYER R. P. Growth of L cell suspensions on a Warburg apparatus. Proc Soc Exp Biol Med. 1963 Apr;112:1027–1030. doi: 10.3181/00379727-112-28243. [DOI] [PubMed] [Google Scholar]
- Radlett P. J., Telling R. C., Stone C. J., Whiteside J. P. Improvements in the growth of BHK-21 cells in submerged culture. Appl Microbiol. 1971 Oct;22(4):534–537. doi: 10.1128/am.22.4.534-537.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SWIM H. E., PARKER R. F. Effect of pluronic F68 on growth fibroblasts in suspension on rotary shaker. Proc Soc Exp Biol Med. 1960 Jan;103:252–254. doi: 10.3181/00379727-103-25477. [DOI] [PubMed] [Google Scholar]