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. 1975 Jul;2(1):55–61. doi: 10.1128/jcm.2.1.55-61.1975

A scheme for the identification of thermophilic actinomycetes associated with hypersensitivity pneumonitis.

V P Kurup, J N Fink
PMCID: PMC274125  PMID: 1225928

Abstract

A scheme has been developed for the identification of thermophilic actinomycetes associated with hypersensitivity pneumonitis. Eighty strains, 10 Micropolyspora faeni, 6 Saccharomonospora viridis, 52 Thermoactinomyces candidus, 7 T. vulgaris, 4 T. sacchari, and 1 T. dichotomica, either isolated from patients' environment or received as authentic strains, were studied. In addition to the cultural and microscopic morphology in various media, each strain was subjected to an array of biochemical tests. These tests included decomposition of tyrosine, xanthine, hypoxanthine, gelatin, casein, esculin, and arbutin. Using a rapid thin-layer chromatography method, the isomer of diaminopimelic acid and sugar in the whole cell hydrolysate were studied. The thermophilic actinomycetes can be identified in a reasonable period of time using a combination of all these tests.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BECKER B., LECHEVALIER M. P., GORDON R. E., LECHEVALIER H. A. RAPID DIFFERENTIATION BETWEEN NOCARDIA AND STREPTOMYCES BY PAPER CHROMATOGRAPHY OF WHOLE-CELL HYDROLYSATES. Appl Microbiol. 1964 Sep;12:421–423. doi: 10.1128/am.12.5.421-423.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Banaszak E. F., Thiede W. H., Fink J. N. Hypersensitivity pneumonitis due to contamination of an air conditioner. N Engl J Med. 1970 Aug 6;283(6):271–276. doi: 10.1056/NEJM197008062830601. [DOI] [PubMed] [Google Scholar]
  3. Barboriak J. J., Fink J. N., Scribner G. Immunologic cross-reactions of thermophilic actinomycetes isolated from home environments. J Allergy Clin Immunol. 1972 Feb;49(2):81–85. doi: 10.1016/0091-6749(72)90059-0. [DOI] [PubMed] [Google Scholar]
  4. Bauer A. W., Kirby W. M., Sherris J. C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Tech Bull Regist Med Technol. 1966 Mar;36(3):49–52. [PubMed] [Google Scholar]
  5. Christensen W. B. Urea Decomposition as a Means of Differentiating Proteus and Paracolon Cultures from Each Other and from Salmonella and Shigella Types. J Bacteriol. 1946 Oct;52(4):461–466. doi: 10.1128/jb.52.4.461-466.1946. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cross T., Maciver A. M., Lacey J. The thermophilic actinomycetes in mouldy hay: Micropolyspora faeni sp.nov. J Gen Microbiol. 1968 Mar;50(3):351–359. doi: 10.1099/00221287-50-3-351. [DOI] [PubMed] [Google Scholar]
  7. Fink J. N., Banaszak E. F., Thiede W. H., Barboriak J. J. Interstitial pneumonitis due to hypersensitivity to an organism contaminating a heating system. Ann Intern Med. 1971 Jan;74(1):80–83. doi: 10.7326/0003-4819-74-1-80. [DOI] [PubMed] [Google Scholar]
  8. GORDON R. E., MIHM J. M. A comparative study of some strains received as nocardiae. J Bacteriol. 1957 Jan;73(1):15–27. doi: 10.1128/jb.73.1.15-27.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. GORDON R. E., SMITH M. M. Proposed group of characters for the separation of Streptomyces and Nocardia. J Bacteriol. 1955 Feb;69(2):147–150. doi: 10.1128/jb.69.2.147-150.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gordon R. E., Horan A. C. Nocardia dassonvillei, a macroscopic replica of Streptomyces griseus. J Gen Microbiol. 1968 Feb;50(2):235–240. doi: 10.1099/00221287-50-2-235. [DOI] [PubMed] [Google Scholar]
  11. Gordon R. E. Some criteria for the recognition of Nocardia madurae (Vincent) Blanchard. J Gen Microbiol. 1966 Nov;45(2):355–364. doi: 10.1099/00221287-45-2-355. [DOI] [PubMed] [Google Scholar]
  12. Kurup P. V., Schmitt J. A. Numerical taxonomy of Nocardia. Can J Microbiol. 1973 Aug;19(8):1035–1048. doi: 10.1139/m73-164. [DOI] [PubMed] [Google Scholar]
  13. Lacey J. Thermoactinomyces sacchari sp. nov., a thermophilic actinomycete causing bagassosis. J Gen Microbiol. 1971 Jun;66(3):327–338. doi: 10.1099/00221287-66-3-327. [DOI] [PubMed] [Google Scholar]
  14. Lechevalier M. P. Identification of aerobic actinomycetes of clinical importance. J Lab Clin Med. 1968 Jun;71(6):934–944. [PubMed] [Google Scholar]
  15. PEPYS J., JENKINS P. A., FESTENSTEIN G. N., GREGORY P. H., LACEY M. E., SKINNER F. A. FARMER'S LUNG. THERMOPHILIC ACTINOMYCETES AS A SOURCE OF "FARMER'S LUNG HAY" ANTIGEN. Lancet. 1963 Sep 21;2(7308):607–611. doi: 10.1016/s0140-6736(63)90398-2. [DOI] [PubMed] [Google Scholar]
  16. PEPYS J., JENKINS P. A. PRECIPITIN (F.L.H.) TEST IN FARMER'S LUNG. Thorax. 1965 Jan;20:21–35. doi: 10.1136/thx.20.1.21. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Seabury J., Salvaggio J., Domer J., Fink J., Kawai T. Characterization of thermophilic actinomycetes isolated from residential heating and humidification systems. J Allergy Clin Immunol. 1973 Mar;51(3):161–173. doi: 10.1016/0091-6749(73)90021-3. [DOI] [PubMed] [Google Scholar]
  18. WENZEL F., EMANUEL D. A., LAWTON B. R., MAGNIN G. E. ISOLATION OF THE CAUSATIVE AGENT OF FARMER'S LUNG. Ann Allergy. 1964 Oct;22:533–540. [PubMed] [Google Scholar]

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