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Time course of inhibition and radical- and 5′-deoxyadenosine-formation.
Reaction of 5,6-LAM (50 μM), 120 μM PLP, 120 μM [adenine-14C]adenosylcobalamin at pH 8.5 (EPPS buffer) was initiated with 20 mM 4-thia-d-lysine. □, Time course for increasing radical spin (μM); ●, time course for the formation of 5′-deoxy[8-14C]adenosine (μM); inset, enzyme activity.
