Abstract
Levels of lymphocytic choriomeningitis virus antibody were assayed in 62 infected persons. The three tests used were indirect fluorescent antibody (IFA), complement fixation, and neutralization in mice. The sera first became positive by the IFA test, and IFA titers rapidly rose to a relatively high level, with the sera remaining positive long after the antibody detectable by complement fixation had disappeared. The IFA test appeared to be specific. The sera became positive last by the mouse neutralization test; with this test, antibody first appeared several weeks after infection. Virus-infected cells were stable when stored at -60 C, allowing diagnostic sera to be tested promptly by the IFA test. The IFA test for lymphocytic choriomeningitis antibody should increase the number of serological diagnoses, since it is not only rapid and specific, but detects cases not diagnosed by the other methods.
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Selected References
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- Cohen S. M., Triandaphilli I. A., Barlow J. L., Hotchin J. Immunofluorescent detection of antibody to lymphocytic choriomeningitis virus in man. J Immunol. 1966 May;96(5):777–784. [PubMed] [Google Scholar]
- Dye L. A., Feorino P. M. Evaluation of two types of infectious mononucleosis antigen slides by the indirect fluorescent-antibody technique. Appl Microbiol. 1973 Feb;25(2):315–316. doi: 10.1128/am.25.2.315-316.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hotchin J., Benson L., Sikora E. The detection of neutralizing antibody to lymphocytic choriomeningitis virus in mice. J Immunol. 1969 May;102(5):1128–1135. [PubMed] [Google Scholar]
- Hotchin J., Kinch W. Microplaque reduction: new assay for neutralizing antibody to lymphocytic choriomeningitis virus. J Infect Dis. 1975 Feb;131(2):186–188. doi: 10.1093/infdis/131.2.186. [DOI] [PubMed] [Google Scholar]
- MACPHERSON I., STOKER M. Polyoma transformation of hamster cell clones--an investigation of genetic factors affecting cell competence. Virology. 1962 Feb;16:147–151. doi: 10.1016/0042-6822(62)90290-8. [DOI] [PubMed] [Google Scholar]
- MEYER H. M., Jr, JOHNSON R. T., CRAWFORD I. P., DASCOMB H. E., ROGERS N. G. Central nervous system syndromes of "vital" etiology. A study of 713 cases. Am J Med. 1960 Aug;29:334–347. doi: 10.1016/0002-9343(60)90029-2. [DOI] [PubMed] [Google Scholar]