Abstract
A presumptive hemagglutination test for the serological diagnosis of leptospirosis in humans is described. The antigen was prepared from a soluble alcohol extract of an andamana strain sorbed to human O-negative erythrocytes and preserved by pyruvic aldehyde fixation. In this study, the overall sensitivity of the hemagglutination test was 92% in contrast to 69% for the presumptive slide agglutination test. The specificity was 95% for the hemagglutination test in comparison with 83% for the slide test.
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Selected References
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- Baker L. A., Cox C. D. Quantitative assay for genus-specific leptospiral antigen and antibody. Appl Microbiol. 1973 Apr;25(4):697–698. doi: 10.1128/am.25.4.697-698.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- COX C. D., ALEXANDER A. D., MURPHY L. C. Evaluation of the hemolytic test in the serodiagnosis of human leptospirosis. J Infect Dis. 1957 Sep-Oct;101(2):210–218. doi: 10.1093/infdis/101.2.210. [DOI] [PubMed] [Google Scholar]
- COX C. D., STOVER R. C., TREICK R. W. Serological studies on hemolytic antigen from Leptospira. Proc Soc Exp Biol Med. 1958 Jun;98(2):265–269. doi: 10.3181/00379727-98-24012. [DOI] [PubMed] [Google Scholar]
- COX C. D. Standardization and stabilization of an extract from Leptospira biflexa and its use in the hemolytic test for leptospirosis. J Infect Dis. 1957 Sep-Oct;101(2):203–209. doi: 10.1093/infdis/101.2.203. [DOI] [PubMed] [Google Scholar]
- Cole J. R., Jr, Sulzer C. R., Pursell A. R. Improved microtechnique for the leptospiral microscopic agglutination test. Appl Microbiol. 1973 Jun;25(6):976–980. doi: 10.1128/am.25.6.976-980.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Imamura S., Matsui H., Ashizawa Y. Studies on indirect hemagglutination test for leptospirosis. Jpn J Exp Med. 1972 Dec;42(6):563–568. [PubMed] [Google Scholar]
- Sulzer C. R., Jones W. L. Evaluation of a hemagglutination test for human leptospirosis. Appl Microbiol. 1973 Nov;26(5):655–657. doi: 10.1128/am.26.5.655-657.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
