Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1975 Nov;2(5):377–381. doi: 10.1128/jcm.2.5.377-381.1975

Rapid separation of immunoglobulin M from immunoglobulin G antibodies for reliable diagnosis of recent rubella infections.

W Frisch-Niggemeyer
PMCID: PMC274194  PMID: 1194404

Abstract

Chromatography on controlled pore glass was adapted for the separation of immunoglobulin M (IgM) and immunoglobulin G (IgG) rubella antibodies from 0.3-ml samples of human serum. An extremely sharp separation of IgM from IgG antibodies could be obtained within 40 min. Nonspecific inhibitors were removed before chromatography by precipitation with high-molecular-weight dextran sulfate, and the titer of rubella antibodies in the different classes of immunoglobulins were assayed with a modified hemagglutination inhibition technique. The combination of these methods is recommended for routine tests. It permits an accurate diagnosis of recent rubella infection within a few hours.

Full text

PDF
377

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Banatvala J. E., Best J. M., Bertrand J., Bowern N. A., Hudson S. M. Serological assessment of rubella during pregnancy. Br Med J. 1970 Aug 1;3(5717):247–250. doi: 10.1136/bmj.3.5717.247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burstein M., Scholnick H. R. Lipoprotein-polyanion-metal interactions. Adv Lipid Res. 1973;11(0):67–108. [PubMed] [Google Scholar]
  3. Bürgin-Wolff A., Hernandez R., Just M. Separation of rubella IgM, IgA, and IgG antibodies by gel filtration on agarose. Lancet. 1971 Dec 11;2(7737):1278–1280. doi: 10.1016/s0140-6736(71)90600-3. [DOI] [PubMed] [Google Scholar]
  4. CLARKE D. H., CASALS J. Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Am J Trop Med Hyg. 1958 Sep;7(5):561–573. doi: 10.4269/ajtmh.1958.7.561. [DOI] [PubMed] [Google Scholar]
  5. CORNWELL D. G., KRUGER F. A. Molecular complexes in the isolation and characterization of plasma lipoproteins. J Lipid Res. 1961 Apr;2:110–134. [PubMed] [Google Scholar]
  6. Freymuth F., Morel C. Mise en évidence des IgM par filtration sur gel Sephadex G 200. Application à la rubéole. Ann Biol Clin (Paris) 1972;30(6):579–583. [PubMed] [Google Scholar]
  7. Frisch-Niggemeyer W., Heinz F., Stemberger H. Eine schnelle und verlässliche methode zur Erkennung frischer bzw. älterer Infektionen mit Rubella-Virus. Immun Infekt. 1974 Nov;2(5):231–237. [PubMed] [Google Scholar]
  8. Fuccillo D. A., Sever J. L. Viral teratology. Bacteriol Rev. 1973 Mar;37(1):19–31. doi: 10.1128/br.37.1.19-31.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gschwender H. H., Haller W., Hofschneider P. H. Large-scale preparations of viruses by steric chromatography on columns of controlled pore glass. Phi-X174, M13, M12, Q-beta and T4 bacteriophages. Biochim Biophys Acta. 1969 Oct 22;190(2):460–469. doi: 10.1016/0005-2787(69)90095-1. [DOI] [PubMed] [Google Scholar]
  10. Gupta J. D., Peterson V., Stout M., Murphy A. M. Single-sample diagnosis of recent rubella by fractionation of antibody on Sephadex G-200 column. J Clin Pathol. 1971 Sep;24(6):547–550. doi: 10.1136/jcp.24.6.547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Haller W., Tympner K. D., Hannig K. Preparation of immunoglobulin concentrates from human serum by chromatography on controlled pore glass. Anal Biochem. 1970 May;35(1):23–31. doi: 10.1016/0003-2697(70)90004-7. [DOI] [PubMed] [Google Scholar]
  12. Haukenes G., Aasen J. Rubella haemagglutination inhibitors: their separation from antibodies. Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;79(5):679–685. doi: 10.1111/j.1699-0463.1971.tb00096.x. [DOI] [PubMed] [Google Scholar]
  13. Hofmann H., Kunz C. Zur serologischen Frühdiagnose von Virusinfektionen. Wien Klin Wochenschr. 1973 Jul 6;85(27):490–493. [PubMed] [Google Scholar]
  14. Horstmann D. M. Rubella: the challenge of its control. J Infect Dis. 1971 Jun;123(6):640–654. doi: 10.1093/infdis/123.6.640. [DOI] [PubMed] [Google Scholar]
  15. Iwakata S., Rhodes A. J., Labzoffsky N. A. The significance of specific IgM antibody in the diagnosis of rubella employing the immunofluorescence technique. Can Med Assoc J. 1972 Feb 19;106(4):327–330. [PMC free article] [PubMed] [Google Scholar]
  16. Kayser F. H. Erfahrungen mit dem Rubeolen-Hämmagglutinationshemmtest. Zentralbl Bakteriol Orig. 1969 Jan;209(2):275–278. [PubMed] [Google Scholar]
  17. Laufs R., Fleckenstein B. Zur serologischen Diagnostik der Rötelnerkrankung während der Schwangerschaft und der Rötelnembryopathie beim Neugeborenen. Zentralbl Bakteriol Orig A. 1972 May;220(1):235–241. [PubMed] [Google Scholar]
  18. Lennette E. H., Schmidt N. J., Magoffin R. L. The hemagglutination inhibition test for rubella: a comparison of its sensitivity to that of neutralization, complement fixation and fluorescent antibody tests for diagnosis of infection and determination of immunity status. J Immunol. 1967 Oct;99(4):785–793. [PubMed] [Google Scholar]
  19. Liebhaber H. Measurement of rubella antibody by hemagglutination inhibition. II. Characteristics of an improved HAI test employing a new method for the removal of non-immunoglobulin HA inhibitors from serum. J Immunol. 1970 Apr;104(4):826–834. [PubMed] [Google Scholar]
  20. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  21. Peetermans J., Huygelen C. L'emploi d'hérmaties de pigeons dans le test d'inhibition de l'hémagglutination de la rubéole. Presse Med. 1967 Oct 21;75(43):2177–2178. [PubMed] [Google Scholar]
  22. Prinzie A. Röteln, Rötelnembryopathie und Rötelnimpfstoff. Dtsch Med Wochenschr. 1971 Aug 20;96(34):1367–1371. doi: 10.1055/s-0028-1110144. [DOI] [PubMed] [Google Scholar]
  23. Schmitz H., Haas R. Determination of different cytomegalovirus immunoglobulins (IgG, IgA, IgM) by immunofluorescence. Arch Gesamte Virusforsch. 1972;37(1):131–140. doi: 10.1007/BF01241158. [DOI] [PubMed] [Google Scholar]
  24. Stewart G. L., Parkman P. D., Hopps H. E., Douglas R. D., Hamilton J. P., Meyer H. M., Jr Rubella-virus hemagglutination-inhibition test. N Engl J Med. 1967 Mar 9;276(10):554–557. doi: 10.1056/NEJM196703092761006. [DOI] [PubMed] [Google Scholar]
  25. TARTAKOW I. J. THE TERATOGENICITY OF MATERNAL RUBELLA. J Pediatr. 1965 Feb;66:380–391. doi: 10.1016/s0022-3476(65)80196-2. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES