Abstract
An indirect solid phase radioimmunoassay (RIA) was developed for typing antibody to herpes simplex virus (HSV) types 1 and 2 (HSV-1 and HSV-2) in human sera. The test is based upon absorption of sera with uninfected, HSV-1-infected cells and testing for residual antibody. The high sensitivity of the RIA method for detecting HSV antibody permits examination of sera at high dilutions, and thus relatively small volumes of virus-infected cells are required for cross-absorption of antibodies. Results obtained in RIA typing of HSV antibodies showed good agreement with those obtained by microneutralization and inhibition of passive hemagglutination. The HSV antibody type(s) determined by RIA also showed good correlation with the virus type isolated from the individual, either from clinical specimens or sensory nerve ganglia. The technique was very sensitive for detection and typing of HSV antibdodies in cerebrospinal fluids. The RIA method was highly suitable for detecting two types of HSV antibody in the same serum specimen, and it was possible to show that a marked, type-specific antibody response to HSV-2 does occur in individuals with a primary HSV-2 infection who have experienced a prior infection with HSV-1.
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Selected References
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- Back A. F., Schmidt N. J. Indirect hemagglutinating antibody response to Herpesvirus hominis types 1 and 2 in immunized laboratory animals and in natural infections of man. Appl Microbiol. 1974 Sep;28(3):392–399. doi: 10.1128/am.28.3.392-399.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Back A. F., Schmidt N. J. Typing Herpesvirus hominis antibodies and isolates by inhibition of the indirect hemagglutination reaction. Appl Microbiol. 1974 Sep;28(3):400–405. doi: 10.1128/am.28.3.400-405.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Baringer J. R. Recovery of herpes simplex virus from human sacral ganglions. N Engl J Med. 1974 Oct 17;291(16):828–830. doi: 10.1056/NEJM197410172911606. [DOI] [PubMed] [Google Scholar]
- Baringer J. R., Swoveland P. Recovery of herpes-simplex virus from human trigeminal ganglions. N Engl J Med. 1973 Mar 29;288(13):648–650. doi: 10.1056/NEJM197303292881303. [DOI] [PubMed] [Google Scholar]
- Bernstein M. T., Stewart J. A. Method for typing antisera to Herpesvirus hominis by indirect hemagglutination inhibition. Appl Microbiol. 1971 Apr;21(4):680–684. doi: 10.1128/am.21.4.680-684.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Forghani B., Schmidt N. J., Lennette E. H. Solid phase radioimmunoassay for identification of Herpesvirus hominis types 1 and 2 from clinical materials. Appl Microbiol. 1974 Oct;28(4):661–667. doi: 10.1128/am.28.4.661-667.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GERLOFF R. K., HOYER B. H., McLAREN L. C. Precipitation of radiolabeled poliovirus with specific antibody and antiglobulin. J Immunol. 1962 Oct;89:559–570. [PubMed] [Google Scholar]
- HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
- Hayashi K., Rosenthal J., Notkins A. L. Iodine-125-labeled antibody to viral antigens: binding to the surface of virus-infected cells. Science. 1972 May 5;176(4034):516–518. doi: 10.1126/science.176.4034.516. [DOI] [PubMed] [Google Scholar]
- Lander J. J., Alter H. J., Purcell R. H. Frequency of antibody to hepatitis-associated antigen as measured by a new radioimmunoassay technique. J Immunol. 1971 May;106(5):1166–1171. [PubMed] [Google Scholar]
- McConahey P. J., Dixon F. J. A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966;29(2):185–189. doi: 10.1159/000229699. [DOI] [PubMed] [Google Scholar]
- Nahmias A. J., Dowdle W. R., Kramer J. H., Luce C. F., Mansour S. C. Antibodies to Herpesvirus hominis types 1 and 2 in the rabbit. J Immunol. 1969 Apr;102(4):956–962. [PubMed] [Google Scholar]
- Plummer G. A review of the identification and titration of antibodies to herpes simplex viruses type 1 and type 2 in human sera. Cancer Res. 1973 Jun;33(6):1469–1476. [PubMed] [Google Scholar]
- Rawls W. E., Iwamoto K., Adam E., Melnick J. L. Measurement of antibodies to herpesvirus types 1 and 2 in human sera. J Immunol. 1970 Mar;104(3):599–606. [PubMed] [Google Scholar]
- Rosenthal J. D., Hayashi K., Notkins A. L. Rapid micro-radioimmunoassay for the measurement of antiviral antibody. J Immunol. 1972 Jul;109(1):171–173. [PubMed] [Google Scholar]
- Smith J. W., Adam E., Melnick J. L., Rawls W. E. Use of the 51 Cr release test to demonstrate patterns of antibody response in humans to herpesvirus types 1 and 2. J Immunol. 1972 Sep;109(3):554–564. [PubMed] [Google Scholar]
- Wiktor T. J., Koprowski H., Dixon F. Radioimmunoassay procedure for rabies binding antibodies. J Immunol. 1972 Sep;109(3):464–470. [PubMed] [Google Scholar]