Abstract
Acetylmethylcarbinol (acetoin) production by Neisseria gonorrhoeae and other Neisseria species was established by gas-liquid chromatography and by mass spectrometric data. Sixty-nine isolates of Neisseria were tested by incubating them in a chemically defined fluid medium. The medium was extracted with organic solvents and derivatized with heptafluorobutryic anhydride for gas chromatography and mass spectrometry. Cultures of 58 of the same strains were tested with the conventional Voges-Proskauer reagents, and results were compared with those of gas-liquid chromatography. When glucose was used as an energy source, N. gonorrhoeae, some N. meningitidis, and N. lactamica produced enough acetoin in 16 h to be detectable by either method, whereas other Neisseria species produce amounts detectable only by gas chromatography. The conventional acetylmethylcarbinol test with the chemically defined medium and maltose as an energy source might be used to develop methods that would differentiate certain members of the genus, including the pathogenic species.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bovre K., Fuglesang J. E., Henriksen S. D. Neisseria elongata. Presentation of new isolates. Acta Pathol Microbiol Scand B Microbiol Immunol. 1972;80(6):919–922. doi: 10.1111/j.0365-5563.1973.tb00020.x. [DOI] [PubMed] [Google Scholar]
- Brooks J. B., Cherry W. B., Thacker L., Alley C. C. Analysis by gas chromatography of amines and nitrosamines produced in vivo and in vitro by Proteus mirabilis. J Infect Dis. 1972 Aug;126(2):143–153. doi: 10.1093/infdis/126.2.143. [DOI] [PubMed] [Google Scholar]
- Brooks J. B., Kellogg D. S., Alley C. C., Short H. B., Handsfield H. H., Huff B. Gas chromatography as a potential means of diagnosing arthritis. I. Differentiation between staphylococcal, streptococcal, gonococcal, and traumatic arthritis. J Infect Dis. 1974 Jun;129(6):660–668. doi: 10.1093/infdis/129.6.660. [DOI] [PubMed] [Google Scholar]
- Brooks J. B., Kellogg D. S., Thacker L., Turner E. M. Analysis by gas chromatography of hydroxy acids produced by several species of Neisseria. Can J Microbiol. 1972 Feb;18(2):157–168. doi: 10.1139/m72-026. [DOI] [PubMed] [Google Scholar]
- Carifo K., Catlin B. W. Neisseria gonorrhoeae auxotyping: differentiation of clinical isolates based on growth responses on chemically defined media. Appl Microbiol. 1973 Sep;26(3):223–230. doi: 10.1128/am.26.3.223-230.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Catlin B. W. Nutritional profiles of Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica in chemically defined media and the use of growth requirements for gonococcal typing. J Infect Dis. 1973 Aug;128(2):178–194. doi: 10.1093/infdis/128.2.178. [DOI] [PubMed] [Google Scholar]
- EDDY B. P. The Voges-Proskauer reaction and its significance: a review. J Appl Bacteriol. 1961 Apr;24:27–41. doi: 10.1111/j.1365-2672.1961.tb00230.x. [DOI] [PubMed] [Google Scholar]
- Vandekerkove M., Faucon R., Audiffren P., Oddou A. Contribution à l'étude pratique du métabolisme des glucides par Neisseria intracellularis. V. Mise en evidence de l'acetyl-methyl-carbinol produit à partir du glucose. Med Trop (Mars) 1965 Jul-Aug;25(4):457–462. [PubMed] [Google Scholar]
- WIXOM R. L. ACETOLACTATE METABOLISM AND THE PRESENCE OF A DIHYDROXY ACID DEHYDRATASE IN MICRO-ORGANISMS. Biochem J. 1965 Feb;94:427–435. doi: 10.1042/bj0940427. [DOI] [PMC free article] [PubMed] [Google Scholar]
- White L. A., Kellogg D. S., Jr An improved fermentation medium for Neisseria gonorrhoeae and other Neisseria. Health Lab Sci. 1965 Oct;2(4):238–241. [PubMed] [Google Scholar]