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. 1976 Jan;3(1):42–46. doi: 10.1128/jcm.3.1.42-46.1976

Evaluation of an adenosine 5'-triphosphate assay as a screening method to detect significant bacteriuria.

D N Alexander, G M Ederer, J M Matsen
PMCID: PMC274223  PMID: 767357

Abstract

The bioluminescent reaction of adenosine 5'-triphosphate (ATP) with luciferin and luciferase has been used in conjunction with a sensitive photometer (Lab-Line's ATP photometer) to detect significant bacteriuria in urine. This rapid method of screening urine specimens for bacteriuria was evaluated by using 348 urine specimens submitted to the clinical microbiology laboratory at the University of Minnesota Hospitals for routine culture using the calibrated loop-streak plate method. There was 89.4% agreement between the culture method and the ATP assay, with 7.0% false positive and 27.0% false negative results from the ATP assay using 10(5) organisms/ml of urine or greater as positive for significant bacteriuria and less than 10(5) organisms/ml as negative for significant bacteriuria.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aledort L. M., Weed R. I., Troup S. B. Ionic effects on firefly bioluminescence assay of red blood cell ATP. Anal Biochem. 1966 Nov;17(2):268–277. doi: 10.1016/0003-2697(66)90205-3. [DOI] [PubMed] [Google Scholar]
  2. Johnson R. A., Hardman J. G., Broadus A. E., Sutherland E. W. Analysis of adenosine 3',5'-monophosphate with luciferase luminescence. Anal Biochem. 1970 May;35(1):91–97. doi: 10.1016/0003-2697(70)90014-x. [DOI] [PubMed] [Google Scholar]
  3. KASS E. H. Asymptomatic infections of the urinary tract. Trans Assoc Am Physicians. 1956;69:56–64. [PubMed] [Google Scholar]
  4. KASS E. H. Bacteriuria and the diagnosis of infections of the urinary tract; with observations on the use of methionine as a urinary antiseptic. AMA Arch Intern Med. 1957 Nov;100(5):709–714. doi: 10.1001/archinte.1957.00260110025004. [DOI] [PubMed] [Google Scholar]
  5. KUNIN C. M. The quantitative significance of bacteria visualized in the unstained urinary sediment. N Engl J Med. 1961 Sep 21;265:589–590. doi: 10.1056/NEJM196109212651207. [DOI] [PubMed] [Google Scholar]
  6. Levin G. V., Usdin E., Slonim A. R. Rapid detection of microorganisms in aerospace water systems. Aerosp Med. 1968 Jan;39(1):14–16. [PubMed] [Google Scholar]
  7. Rasmussen H., Nielsen R. An improved analysis of adenosine triphosphate by the luciferase method. Acta Chem Scand. 1968;22(6):1745–1756. doi: 10.3891/acta.chem.scand.22-1745. [DOI] [PubMed] [Google Scholar]
  8. Stanley P. E., Williams S. G. Use of the liquid scintillation spectrometer for determining adenosine triphosphate by the luciferase enzyme. Anal Biochem. 1969 Jun;29(3):381–392. doi: 10.1016/0003-2697(69)90323-6. [DOI] [PubMed] [Google Scholar]
  9. Van Dyke K., Stitzel R., McClellan T., Szustkiewicz C. An automated procedure for the sensitive and specific determination of ATP. Clin Chem. 1969 Jan;15(1):3–14. [PubMed] [Google Scholar]

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