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. 1976 Feb;3(2):81–85. doi: 10.1128/jcm.3.2.81-85.1976

Gas chromatographic characterization of mycobacteria: analysis of fatty acids and trifluoroacetylated whole-cell methanolysates.

L Larsson, P A Mårdh
PMCID: PMC274238  PMID: 815272

Abstract

Mycobacterium avium, M. bovis strain Bacillus Calmette-Guerin (BCG), M. kansasii, and M. tuberculosis were studied by gas-liquid chromatography. Methylated fatty acids of the mycobacterial lipids and trifluoroacetyl (TFA) derivatives of whole-cell methanolysates were analyzed. Both the fatty acid and the TFA chromatograms showed reproducible differences between the various mycobacteria studied. Chromatograms from different strains of one and the same species showed negligible differences. Fatty acid methyl esters and TFA methyl glycosides are probably the main constituents of the TFA chromatograms. TFA derivatives are easily prepared and the method provides a potential tool for species indentification of mycobacteria.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Acharya P. V., Goldman D. S. Chemical composition of the cell wall of the H37Ra strain of Mycobacterium tuberculosis. J Bacteriol. 1970 Jun;102(3):733–739. doi: 10.1128/jb.102.3.733-739.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ando S., Yamakawa T. Application of trifluoroacetyl derivatives to sugar and lipid chemistry. I. Gas chromatographic analysis of common constituents of glycolipids. J Biochem. 1971 Aug;70(2):335–340. doi: 10.1093/oxfordjournals.jbchem.a129645. [DOI] [PubMed] [Google Scholar]
  3. Azuma I., Ouchida A., Taniyama T., Yamamura Y., Shoji K. The mycolic acids of Mycobacterium rhodochrous and Nocardia corallina. Biken J. 1974 Mar;17(1):1–9. [PubMed] [Google Scholar]
  4. Farshtchi D., Moss C. W. Characterization of bacteria by gas chromatography: comparison of trimethylsilyl derivatives of whole-cell hydrolysates. Appl Microbiol. 1969 Feb;17(2):262–267. doi: 10.1128/am.17.2.262-267.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Farshy D. C., Thomas M. L., Moss C. W. Characterization of treponemes by gas chromatography. Br J Vener Dis. 1970 Dec;46(6):441–444. doi: 10.1136/sti.46.6.441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fontanges R., Blandenet G., Queignec R. Difficultés d'application de la chromatographie en phase gazeuse à l'identification des bactéries. Ann Inst Pasteur (Paris) 1967 Jan;112(1):10–23. [PubMed] [Google Scholar]
  7. Froholm L. O., Jantzen E., Hytta R., Bovre K. Gas chromatography of bacterial whole cell methanolysates. II. A taxonomic evaluation of the method for species of Moraxella. Acta Pathol Microbiol Scand B Microbiol Immunol. 1972;80(5):672–682. [PubMed] [Google Scholar]
  8. Jantzen E., Froholm L. O., Hytta R., Bovre K. Gas chromatography of bacterial whole cell methanolysates. I. The usefulness of trimethylsilyl- and trifluoroacetyl derivatives for strain and species characterization. Acta Pathol Microbiol Scand B Microbiol Immunol. 1972;80(5):660–671. [PubMed] [Google Scholar]
  9. Kanetsuna F. Chemical analyses of mycobacterial cell walls. Biochim Biophys Acta. 1968 Apr 16;158(1):130–143. doi: 10.1016/0304-4165(68)90080-9. [DOI] [PubMed] [Google Scholar]
  10. Lucchesi M., Cattaneo C., De Ritis G. C. The chromatographic separation of fatty acids (C11-C2O) from mycobacteria. Bull Int Union Tuberc. 1967 Jun;39(1):65–70. [PubMed] [Google Scholar]
  11. Misaki A., Yukawa S. Studies on cell walls of Mycobacteria. II. Constitution of polysaccharides from BCG cell walls. J Biochem. 1966 May;59(5):511–520. doi: 10.1093/oxfordjournals.jbchem.a128335. [DOI] [PubMed] [Google Scholar]
  12. Reiner E., Beam R. E., Kubica G. P. Pyrolysis-gas-liquid chromatography studies for the classification of mycobacteria. Am Rev Respir Dis. 1969 May;99(5):750–759. doi: 10.1164/arrd.1969.99.5.750. [DOI] [PubMed] [Google Scholar]
  13. Reiner E., Hicks J. J., Beam R. E., David H. L. Recent studies on mycobacterial differentiation by means of pyrolysis-gas-liquid chromatography. Am Rev Respir Dis. 1971 Nov;104(5):656–660. doi: 10.1164/arrd.1971.104.5.656. [DOI] [PubMed] [Google Scholar]
  14. Reiner E. Identification of bacterial strains by pyrolysis-gas-liquid chromatography. Nature. 1965 Jun 19;206(990):1272–1273. doi: 10.1038/2061272b0. [DOI] [PubMed] [Google Scholar]
  15. Reiner E., Kubica G. P. Predictive value of pyrolysis-gas-liquid chromatography in the differentiation of mycobacteria. Am Rev Respir Dis. 1969 Jan;99(1):42–49. doi: 10.1164/arrd.1969.99.1.42. [DOI] [PubMed] [Google Scholar]
  16. Thoen C. O., Karlson A. G., Ellefson R. D. Comparison by gas-liquid chromatography of the fatty acids acids of Mycobacterium avium and some other nonphotochromogenic mycobacteria. Appl Microbiol. 1971 Oct;22(4):560–563. doi: 10.1128/am.22.4.560-563.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Zanetta J. P., Breckenridge W. C., Vincendon G. Analysis of monosaccharides by gas-liquid chromatography of the O-methyl glycosides as trifluoroacetate derivatives. Application to glycoproteins and glycolipids. J Chromatogr. 1972 Jul 5;69(2):291–304. doi: 10.1016/s0021-9673(00)92897-8. [DOI] [PubMed] [Google Scholar]

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