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. 1976 Feb;3(2):91–95. doi: 10.1128/jcm.3.2.91-95.1976

Plaque assay of Sendai virus in monolayers of a clonal line of porcine kidney cells.

H Ito
PMCID: PMC274240  PMID: 176175

Abstract

The MN strain of Sendai virus formed distinct plaques in monolayers of PS-Y15 cells, an established porcine kidney cell line. The plaque-forming ability was neutralized by specific antibody to the virus. A linear relationship was found between the concentration of virus and the number of plaques. The sensitivity of this assay was about equal to that of the in ovo titration. When applied to the serum neutralization test, the end points obtained were comparable to those of the hemagglutination-inhibition and complement-fixation tests.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Darlington R. W., Portner A., Kingsbury D. W. Sendai virus replication: an ultrastructural comparison of productive and abortive infections in avian cells. J Gen Virol. 1970 Dec;9(3):169–177. doi: 10.1099/0022-1317-9-3-169. [DOI] [PubMed] [Google Scholar]
  2. FUKUMI H., NISHIKAWA F., KITAYAMA T. A pneumotropic virus from mice causing hemagglutination. Jpn J Med Sci Biol. 1954 Aug;7(4):345–363. doi: 10.7883/yoken1952.7.345. [DOI] [PubMed] [Google Scholar]
  3. GRESSER I., ENDERS J. F. The effect of trypsin on representative myxoviruses. Virology. 1961 Apr;13:420–426. doi: 10.1016/0042-6822(61)90273-2. [DOI] [PubMed] [Google Scholar]
  4. HARRIS H., WATKINS J. F. HYBRID CELLS DERIVED FROM MOUSE AND MAN: ARTIFICIAL HETEROKARYONS OF MAMMALIAN CELLS FROM DIFFERENT SPECIES. Nature. 1965 Feb 13;205:640–646. doi: 10.1038/205640a0. [DOI] [PubMed] [Google Scholar]
  5. Inoue Y. K., Yamada M. A. Clonal line of porcine kidney stable cells for assay of Japanese encephalitis virus. J Bacteriol. 1964 May;87(5):1239–1240. doi: 10.1128/jb.87.5.1239-1240.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. NAGATA I., MAENO K., YOSHII S., MATSUMOTO T. PLAQUE FORMATION BY HVJ IN CALF KIDNEY CELLS. (BRIEF REPORT). Arch Gesamte Virusforsch. 1965;15:257–259. doi: 10.1007/BF01257738. [DOI] [PubMed] [Google Scholar]
  7. OKADA Y., TADOKORO J. THE DISTRIBUTION OF CELL FUSION CAPACITY AMONG SEVERAL CELL STRAINS OR CELLS CAUSED BY HVJ. Exp Cell Res. 1963 Dec;32:417–430. doi: 10.1016/0014-4827(63)90182-4. [DOI] [PubMed] [Google Scholar]
  8. SEVER J. L. Application of a microtechnique to viral serological investigations. J Immunol. 1962 Mar;88:320–329. [PubMed] [Google Scholar]
  9. SHIGETA S. PLAQUE FORMATION AND GROWTH CHARACTERISTICS OF SENDAI VIRUS IN CHICK KIDNEY CELL CULTURES. Tohoku J Exp Med. 1964 Jul 25;83:114–120. doi: 10.1620/tjem.83.114. [DOI] [PubMed] [Google Scholar]
  10. Schneeberger E. E., Harris H. An ultrastructural study of inter-specific cell fusion induced by inactivated Sendai virus. J Cell Sci. 1966 Dec;1(4):401–406. doi: 10.1242/jcs.1.4.401. [DOI] [PubMed] [Google Scholar]
  11. Shibuta H., Akami M., Matumoto M. Plaque formation by sendai virus of parainfluenza virus group, type 1 on monkey, calf kidney and chick embryo cell monolayers. Jpn J Microbiol. 1971 Mar;15(2):175–183. doi: 10.1111/j.1348-0421.1971.tb00567.x. [DOI] [PubMed] [Google Scholar]
  12. Shibuta H. Effect of trypsin on the infectivity of Sendai virus grown in several host cells. Jpn J Microbiol. 1972 May;16(3):193–198. doi: 10.1111/j.1348-0421.1972.tb00648.x. [DOI] [PubMed] [Google Scholar]
  13. Shimizu K., Shimizu Y. K., Koama T., Ishida N. Isolation and characterization of two distinct types of HVJ (Sendai virus) spikes. Virology. 1974 Nov;62(1):90–101. doi: 10.1016/0042-6822(74)90305-5. [DOI] [PubMed] [Google Scholar]
  14. van Nunen M. C., van der Veen J. Experimental infection with Sendai virus in mice. Arch Gesamte Virusforsch. 1967;22(3):388–397. doi: 10.1007/BF01242959. [DOI] [PubMed] [Google Scholar]
  15. van der Veen J., Poort Y., Birchfield D. J. Experimental transmission of Sendai virus infection in mice. Arch Gesamte Virusforsch. 1970;31(3):237–246. doi: 10.1007/BF01253758. [DOI] [PubMed] [Google Scholar]

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