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. 1976 Feb;3(2):110–112. doi: 10.1128/jcm.3.2.110-112.1976

Cholesterol requirement of mycoplasmas as determined by microtiter test using polyene antibiotics.

M W Grabowski, S Rottem, M F Barile
PMCID: PMC274244  PMID: 1254708

Abstract

A microtiter metabolic inhibition test was used to determine the effect of filipin and lucensomycin on the growth of representative species of Mycoplasma and Acholeplasma. The cholesterol-requiring species tested were found to be very susceptible to the two antibiotics, whereas the cholesterol nonrequiring species were not.The utilization of this method for differentiation between the Mycoplasma and Acholeplasma species is suggested and its advantages are discussed.

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Selected References

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  1. BYRNE R. J., FRENCH G. R., YANCEY F. S., GOCHENOUR W. S., RUSSELL P. K., RAMSBURG H. H., BRAND O. A., SCHEIDER F. G., BUESCHER E. L. CLINICAL AND IMMUNOLOGIC INTERRELATIONSHIP AMONG VENEZUELAN, EASTERN, AND WESTERN EQUINE ENCEPHALOMYELITIS VIRUSES IN BURROS. Am J Vet Res. 1964 Jan;25:24–31. [PubMed] [Google Scholar]
  2. CASALS J. RELATIONSHIPS AMONG ARTHROPOD-BORNE ANIMAL VIRUSES DETERMINED BY CROSS-CHALLENGE TESTS. Am J Trop Med Hyg. 1963 Jul;12:587–596. doi: 10.4269/ajtmh.1963.12.587. [DOI] [PubMed] [Google Scholar]
  3. CROWLE A. J. A simplified micro double-diffusion agar precipitin technique. J Lab Clin Med. 1958 Nov;52(5):784–787. [PubMed] [Google Scholar]
  4. Calisher C. H., Maness K. S. Arbovirus identification by an agar-gel diffusion technique. Appl Microbiol. 1970 Apr;19(4):557–564. doi: 10.1128/am.19.4.557-564.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cole F. E., Jr, McKinney R. W. Cross-protection in hamsters immunized with group A arbovirus vaccines. Infect Immun. 1971 Jul;4(1):37–43. doi: 10.1128/iai.4.1.37-43.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DULBECCO R., VOGT M., STRICKLAND A. G. A study of the basic aspects of neutralization of two animal viruses, western equine encephalitis virus and poliomyelitis virus. Virology. 1956 Apr;2(2):162–205. doi: 10.1016/0042-6822(56)90017-4. [DOI] [PubMed] [Google Scholar]
  7. Dalrymple J. M., Vogel S. N., Teramoto A. Y., Russell P. K. Antigenic components of group A arbovirus virions. J Virol. 1973 Nov;12(5):1034–1042. doi: 10.1128/jvi.12.5.1034-1042.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Edward D. G. Determination of sterol requirement for Mycoplasmatales. J Gen Microbiol. 1971 Dec;69(2):205–210. doi: 10.1099/00221287-69-2-205. [DOI] [PubMed] [Google Scholar]
  9. Freundt E. A., Andrews B. E., Erno H., Kunze M., Black F. T. The sensitivity of mycoplasmatales to sodium-polyanethol-sulfonate and digitonin. Zentralbl Bakteriol Orig A. 1973 Oct;225(1):104–112. [PubMed] [Google Scholar]
  10. HEARN H. J., Jr Cross-protection between Venezuelan equine encephalomyelitis and eastern equine encephalomyelitis virus. Proc Soc Exp Biol Med. 1961 Jul;107:607–610. doi: 10.3181/00379727-107-26702. [DOI] [PubMed] [Google Scholar]
  11. Hawkes R. A., Marshall I. D. Studies of arboviruses by agar gel diffusion. Am J Epidemiol. 1967 Jul;86(1):28–44. doi: 10.1093/oxfordjournals.aje.a120732. [DOI] [PubMed] [Google Scholar]
  12. Hebert G. A., Pelham P. L., Pittman B. Determination of the optimal ammonium sulfate concentration for the fractionation of rabbit, sheep, horse, and goat antisera. Appl Microbiol. 1973 Jan;25(1):26–36. doi: 10.1128/am.25.1.26-36.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Karabatsos N. Antigenic relationships of group A arboviruses by plaque reduction neutralization testing. Am J Trop Med Hyg. 1975 May;24(3):527–532. doi: 10.4269/ajtmh.1975.24.527. [DOI] [PubMed] [Google Scholar]
  14. LAMPEN J. O., GILL J. W., ARNOW P. M., MAGANA-PLAZA I. INHIBITION OF THE PLEUROPNEUMONIA-LIKE ORGANISM MYCOPLASMA GALLISEPTICUM BY CERTAIN POLYENE ANTIFUNGAL ANTIBIOTICS. J Bacteriol. 1963 Nov;86:945–949. doi: 10.1128/jb.86.5.945-949.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. MCKINNEY R. W., BERGE T. O., SAWYER W. D., TIGERTT W. D., CROZIER D. USE OF AN ATTENUATED STRAIN OF VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS FOR IMMUNIZATION IN MAN. Am J Trop Med Hyg. 1963 Jul;12:597–603. doi: 10.4269/ajtmh.1963.12.597. [DOI] [PubMed] [Google Scholar]
  17. Mussgay M., Weiland E. Preparation of inactivated vaccines against alphaviruses using Semliki Forest virus-white mouse as a model. I. Inactivation experiments and evaluation of double inactivated subunit vaccines. Intervirology. 1973;1(4):259–268. doi: 10.1159/000148854. [DOI] [PubMed] [Google Scholar]
  18. Pedersen C. E., Jr, Marker S. C., Eddy G. A. Comparative electrophoretic studies on the structural proteins of selected group A arboviruses. Virology. 1974 Jul;60(1):312–314. doi: 10.1016/0042-6822(74)90392-4. [DOI] [PubMed] [Google Scholar]
  19. Pedersen C. E., Jr, Slocum D. R., Eddy G. A. Immunological studies on the envelope component of Venezuelan equine encephalomyelitis virus. Infect Immun. 1973 Dec;8(6):901–906. doi: 10.1128/iai.8.6.901-906.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Purcell R. H., Taylor-Robinson D., Wong D. C., Chanock R. M. A color test for the measurement of antibody to the non-acid-forming human Mycoplasma species. Am J Epidemiol. 1966 Jul;84(1):51–66. doi: 10.1093/oxfordjournals.aje.a120627. [DOI] [PubMed] [Google Scholar]
  21. Randall R., Mills J. W. FATAL ENCEPHALITIS IN MAN DUE TO THE VENEZUELAN VIRUS OF EQUINE ENCEPHALOMYELITIS IN TRINIDAD. Science. 1944 Mar 17;99(2568):225–226. doi: 10.1126/science.99.2568.225. [DOI] [PubMed] [Google Scholar]
  22. Razin S., Tully J. G. Cholesterol requirement of mycoplasmas. J Bacteriol. 1970 May;102(2):306–310. doi: 10.1128/jb.102.2.306-310.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Robinson D. M., Berman S., Lowenthal J. P. Mouse potency assay for Western equine encephalomyelitis vaccines. Appl Microbiol. 1972 Jan;23(1):104–107. doi: 10.1128/am.23.1.104-107.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. WEBER M. M., KINSKY S. C. EFFECT OF CHOLESTEROL ON THE SENSITIVITY OF MYCOPLASMA LAIDLAWII TO THE POLYENE ANTIBIOTIC FILIPIN. J Bacteriol. 1965 Feb;89:306–312. doi: 10.1128/jb.89.2.306-312.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. de Kruijff B., Gerritsen W. J., Oerlemans A., Demel R. A., van Deenen L. L. Polyene antibiotic-sterol interactions in membranes of Acholeplasma laidlawii cells and lecithin liposomes. I. Specificity of the membrane permeability changes induced by the polyene antibiotics. Biochim Biophys Acta. 1974 Feb 26;339(1):30–43. doi: 10.1016/0005-2736(74)90330-7. [DOI] [PubMed] [Google Scholar]

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