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. 1976 Feb;3(2):113–118. doi: 10.1128/jcm.3.2.113-118.1976

Preparation and testing of vaccines prepared from the envelopes of Venezuelan, eastern, and western equine encephalomyelitis viruses.

C E Pedersen Jr
PMCID: PMC274245  PMID: 942635

Abstract

Envelope components were separated from Venezuelan, Eastern, and Western equine encephalomyelitis viruses after treatment of the virions with detergent. Vaccines prepared from the envelope component were capable of stimulating mice to produce humoral antibodies. Protective efficacy studies were performed using mono-, di-, and trivalent vaccine combinations. These elicited varying degrees of homologous protection, and Eastern and Venezuelan equine encephalomyelitis envelope products appeared to confer protection to mice challenged with Western equine encephalomyelitis virus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BYRNE R. J., FRENCH G. R., YANCEY F. S., GOCHENOUR W. S., RUSSELL P. K., RAMSBURG H. H., BRAND O. A., SCHEIDER F. G., BUESCHER E. L. CLINICAL AND IMMUNOLOGIC INTERRELATIONSHIP AMONG VENEZUELAN, EASTERN, AND WESTERN EQUINE ENCEPHALOMYELITIS VIRUSES IN BURROS. Am J Vet Res. 1964 Jan;25:24–31. [PubMed] [Google Scholar]
  2. CASALS J. RELATIONSHIPS AMONG ARTHROPOD-BORNE ANIMAL VIRUSES DETERMINED BY CROSS-CHALLENGE TESTS. Am J Trop Med Hyg. 1963 Jul;12:587–596. doi: 10.4269/ajtmh.1963.12.587. [DOI] [PubMed] [Google Scholar]
  3. CROWLE A. J. A simplified micro double-diffusion agar precipitin technique. J Lab Clin Med. 1958 Nov;52(5):784–787. [PubMed] [Google Scholar]
  4. Calisher C. H., Maness K. S. Arbovirus identification by an agar-gel diffusion technique. Appl Microbiol. 1970 Apr;19(4):557–564. doi: 10.1128/am.19.4.557-564.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cole F. E., Jr, McKinney R. W. Cross-protection in hamsters immunized with group A arbovirus vaccines. Infect Immun. 1971 Jul;4(1):37–43. doi: 10.1128/iai.4.1.37-43.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DULBECCO R., VOGT M., STRICKLAND A. G. A study of the basic aspects of neutralization of two animal viruses, western equine encephalitis virus and poliomyelitis virus. Virology. 1956 Apr;2(2):162–205. doi: 10.1016/0042-6822(56)90017-4. [DOI] [PubMed] [Google Scholar]
  7. Dalrymple J. M., Vogel S. N., Teramoto A. Y., Russell P. K. Antigenic components of group A arbovirus virions. J Virol. 1973 Nov;12(5):1034–1042. doi: 10.1128/jvi.12.5.1034-1042.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HEARN H. J., Jr Cross-protection between Venezuelan equine encephalomyelitis and eastern equine encephalomyelitis virus. Proc Soc Exp Biol Med. 1961 Jul;107:607–610. doi: 10.3181/00379727-107-26702. [DOI] [PubMed] [Google Scholar]
  9. Hawkes R. A., Marshall I. D. Studies of arboviruses by agar gel diffusion. Am J Epidemiol. 1967 Jul;86(1):28–44. doi: 10.1093/oxfordjournals.aje.a120732. [DOI] [PubMed] [Google Scholar]
  10. Hebert G. A., Pelham P. L., Pittman B. Determination of the optimal ammonium sulfate concentration for the fractionation of rabbit, sheep, horse, and goat antisera. Appl Microbiol. 1973 Jan;25(1):26–36. doi: 10.1128/am.25.1.26-36.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Karabatsos N. Antigenic relationships of group A arboviruses by plaque reduction neutralization testing. Am J Trop Med Hyg. 1975 May;24(3):527–532. doi: 10.4269/ajtmh.1975.24.527. [DOI] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. MCKINNEY R. W., BERGE T. O., SAWYER W. D., TIGERTT W. D., CROZIER D. USE OF AN ATTENUATED STRAIN OF VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS FOR IMMUNIZATION IN MAN. Am J Trop Med Hyg. 1963 Jul;12:597–603. doi: 10.4269/ajtmh.1963.12.597. [DOI] [PubMed] [Google Scholar]
  14. Mussgay M., Weiland E. Preparation of inactivated vaccines against alphaviruses using Semliki Forest virus-white mouse as a model. I. Inactivation experiments and evaluation of double inactivated subunit vaccines. Intervirology. 1973;1(4):259–268. doi: 10.1159/000148854. [DOI] [PubMed] [Google Scholar]
  15. Pedersen C. E., Jr, Marker S. C., Eddy G. A. Comparative electrophoretic studies on the structural proteins of selected group A arboviruses. Virology. 1974 Jul;60(1):312–314. doi: 10.1016/0042-6822(74)90392-4. [DOI] [PubMed] [Google Scholar]
  16. Pedersen C. E., Jr, Slocum D. R., Eddy G. A. Immunological studies on the envelope component of Venezuelan equine encephalomyelitis virus. Infect Immun. 1973 Dec;8(6):901–906. doi: 10.1128/iai.8.6.901-906.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Robinson D. M., Berman S., Lowenthal J. P. Mouse potency assay for Western equine encephalomyelitis vaccines. Appl Microbiol. 1972 Jan;23(1):104–107. doi: 10.1128/am.23.1.104-107.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

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