Abstract
A rapid medium for the detection of lysine and ornithine decarboxylase and arginine dihydrolase activity of 439 strains of gram-negative, nonfermenting bacteria was evaluated and compared with Moeller decarboxylase medium. Results were obtained in 4 to 24 h using the rapid medium, whereas Moeller medium often required extended (3 to 7 days) incubation. There was 100% agreement in the lysine tests with both media and almost 100% agreement in the ornithine tests. There was 91% agreement in the arginine tests, with the significance of discrepant results discussed. The sensitivity, specificity, and quick results obtained by the rapid test make it a suitable substitute for Moeller medium for the identication of gram-negative, nonfermenting bacteria.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brooker D. C., Lund M. E., Blazevic D. J. Rapid test for lysine decarboxylase activity in Enterobacteriaceae. Appl Microbiol. 1973 Oct;26(4):622–623. doi: 10.1128/am.26.4.622-623.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fay G. D., Barry A. L. Rapid ornithine decarboxylase test for the identification of enterobacteriaceae. Appl Microbiol. 1972 Apr;23(4):710–713. doi: 10.1128/am.23.4.710-713.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gilardi G. L., Hirschl S., Mandel M. Characteristics of yellow-pigmented nonfermentative bacilli (groups VE-1 and VE-2) encountered in clinical bacteriology. J Clin Microbiol. 1975 Apr;1(4):384–389. doi: 10.1128/jcm.1.4.384-389.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gilardi G. L. Nonfermentative gram-negative bacteria encountered in clinical specimens. Antonie Van Leeuwenhoek. 1973;39(2):229–242. doi: 10.1007/BF02578855. [DOI] [PubMed] [Google Scholar]
- LEIFSON E. Staining, shape and arrangement of bacterial flagella. J Bacteriol. 1951 Oct;62(4):377–389. doi: 10.1128/jb.62.4.377-389.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lambert M. A., Moss C. W. Use of gas chromatography for detecting ornithine and lysine decarboxylase activity in bacteria. Appl Microbiol. 1973 Oct;26(4):517–520. doi: 10.1128/am.26.4.517-520.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MØLLER V. Simplified tests for some amino acid decarboxylases and for the arginine dihydrolase system. Acta Pathol Microbiol Scand. 1955;36(2):158–172. doi: 10.1111/j.1699-0463.1955.tb04583.x. [DOI] [PubMed] [Google Scholar]
- Palleroni N. J., Doudoroff M., Stanier R. Y., Solánes R. E., Mandel M. Taxonomy of the aerobic pseudomonads: the properties of the Pseudomonas stutzeri group. J Gen Microbiol. 1970 Feb;60(2):215–231. doi: 10.1099/00221287-60-2-215. [DOI] [PubMed] [Google Scholar]
- Williams G. A., Blazevic D. J., Ederer G. M. Detection of arginine dihydrolase in nonfermentative gram-negative bacteria by use of thin-layer chromatography. Appl Microbiol. 1971 Dec;22(6):1135–1137. doi: 10.1128/am.22.6.1135-1137.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]