Abstract
Since many contaminating nonpathogenic fungi resemble Coccidioides immitis culturally, isolates from patients must be inoculated into laboratory animals to confirem identification as C. immitis. This procedure is time-consuming, expensive, and not generally available in clinical laboratories. When cultures of C. immitis were grown in slide cultures on modified Converse liquid medium in purified agar and incubated at 40 C in a candle jar, all 57 isolates demonstrated inhibition of mycelial growth and conversion of arthrospores to endosporulating spherules in 3 to 5 days. Representative isolates of six species of nonpathogenic fungi that resembled C. immitis culturally either did not grow or failed to produce endosporulating spherules under the same conditions. This procedure is recommended for confirming the identication of a culture as C. immitis.
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- BRESLAU A. M., KUBOTA M. Y. CONTINUOUS IN VITRO CULTIVATION OF SPHERULES OF COCCIDIOIDES IMMITIS. J Bacteriol. 1964 Feb;87:468–472. doi: 10.1128/jb.87.2.468-472.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brosbe E. A. Use of refined agar for the in vitro propagation of the spherule phase of Coccidioides immitis. J Bacteriol. 1967 Jan;93(1):497–498. doi: 10.1128/jb.93.1.497-498.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CONVERSE J. L. Effect of physico-chemical environment of spherulation of Coccidioides immitis in a chemically defined medium. J Bacteriol. 1956 Dec;72(6):784–792. doi: 10.1128/jb.72.6.784-792.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CONVERSE J. L. Growth of spherules of Coccidioides immitis in a chemically defined liquid medium. Proc Soc Exp Biol Med. 1955 Dec;90(3):709–711. doi: 10.3181/00379727-90-22144. [DOI] [PubMed] [Google Scholar]
- EDWARDS P. Q., PALMER C. E. Prevalence of sensitivity to coccidioidin, with special reference to specific and nonspecific reactions to coccidioidin and to histoplasmin. Dis Chest. 1957 Jan;31(1):35–60. doi: 10.1378/chest.31.1.35. [DOI] [PubMed] [Google Scholar]
- FRIEDMAN L., PAPAGIANIS D., BERMAN R. J., SMITH C. E. Studies on Coccidioides immitis: morphology and sporulation capacity of forty-seven strains. J Lab Clin Med. 1953 Sep;42(3):438–444. [PubMed] [Google Scholar]
- LEVINE H. B., COBB J. M., SMITH C. E. Immunity to coccidioi-domycosis induced in mice by purified spherule, arthrospore, and mycelial vaccines. Trans N Y Acad Sci. 1960 Apr;22:436–449. doi: 10.1111/j.2164-0947.1960.tb00711.x. [DOI] [PubMed] [Google Scholar]
- LONES G. W., PEACOCK C. L. Role of carbon dioxide in the dimorphism of Coccidioides immitis. J Bacteriol. 1960 Feb;79:308–309. doi: 10.1128/jb.79.2.308-309.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pappagianis D. Epidemiological aspects of respiratory mycotic infections. Bacteriol Rev. 1967 Mar;31(1):25–34. doi: 10.1128/br.31.1.25-34.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roberts J. A., Counts J. M., Crecelius H. G. Production in vitro of Coccidoides immitis spherules and endospores as a diagnostic aid. Am Rev Respir Dis. 1970 Nov;102(5):811–813. doi: 10.1164/arrd.1970.102.5.811. [DOI] [PubMed] [Google Scholar]