Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1976 Feb;3(2):209–210. doi: 10.1128/jcm.3.2.209-210.1976

Inactivation of viruses in serum with binary ethyleneimine.

H G Bahnemann
PMCID: PMC274262  PMID: 3517

Abstract

The inactivation os six strains from three different groups of viruses with 0.001 M binary ethyleneimine at 37 C proceeded at the same rate in either bovine serum or cell culture medium. The inactivant did not impair the growth-promoting capacity of bovine serum used in cell culture, nor did it affect the antibody activity of guinea pig hyperimmune serum.

Full text

PDF
209

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bahnemann H. G. Binary ethylenimine as an inactivant for foot-and-mouth disease virus and its application for vaccine production. Arch Virol. 1975;47(1):47–56. doi: 10.1007/BF01315592. [DOI] [PubMed] [Google Scholar]
  2. Chang T. H., Elequin F. T. Induction of chromosome aberrations in cultured human cells by ethylenimine and its relation to cell cycle. Mutat Res. 1967 Feb;4(1):83–89. doi: 10.1016/0027-5107(67)90112-1. [DOI] [PubMed] [Google Scholar]
  3. Chu F. C., Johnson J. B., Orr H. C., Probst P. G., Petricciani J. C. Bacterial virus contamination of fetal bovine sera. In Vitro. 1973 Jul-Aug;9(1):31–34. doi: 10.1007/BF02615986. [DOI] [PubMed] [Google Scholar]
  4. Kniazeff A. J. Comments on use of cell cultures in vaccine production. J Am Vet Med Assoc. 1970 Jun 15;156(12):1795–1797. [PubMed] [Google Scholar]
  5. Merril C. R., Friedman T. B., Attallah A. F., Geier M. R., Krell K., Yarkin R. Isolation of bacteriophages from commercial sera. In Vitro. 1972 Sep-Oct;8(2):91–93. doi: 10.1007/BF02615965. [DOI] [PubMed] [Google Scholar]
  6. Molander C. W., Kniazeff A. J., Boone C. W., Paley A., Imagawa D. T. Isolation and characterization of viruses from fetal calf serum. In Vitro. 1971 Nov-Dec;7(3):168–173. doi: 10.1007/BF02617962. [DOI] [PubMed] [Google Scholar]
  7. Nöbel B., Glathe H. Isolierung eines Fremdvirus aus Kälberserum. Arch Exp Veterinarmed. 1974 Mar;28(3):317–322. [PubMed] [Google Scholar]
  8. RAFTERY M. A., COLE R. D. Tryptic cleavage at cysteinyl peptide bonds. Biochem Biophys Res Commun. 1963 Mar 25;10:467–472. doi: 10.1016/0006-291x(63)90381-4. [DOI] [PubMed] [Google Scholar]
  9. STOKER M., MACPHERSON I. SYRIAN HAMSTER FIBROBLAST CELL LINE BHK21 AND ITS DERIVATIVES. Nature. 1964 Sep 26;203:1355–1357. doi: 10.1038/2031355a0. [DOI] [PubMed] [Google Scholar]
  10. Stephan W., Haug E. Unterschiedliche Wirksamkeit von beta-Propiolacton bei der Sterilisation von esterase-aktiven und -inaktiven Seren und Plasmen. Zentralbl Bakteriol Orig. 1970;215(3):381–385. [PubMed] [Google Scholar]
  11. Swack N. S., Fong C. K., Hsiung G. D., Gross P. A. Methods for the detection of viruses in bovine serum. J Clin Microbiol. 1975 Feb;1(2):212–218. doi: 10.1128/jcm.1.2.212-218.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES