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. 1976 Mar;3(3):264–271. doi: 10.1128/jcm.3.3.264-271.1976

Epidemiology of Pseudomonas aeruginosa infections: determination by pyocin typing.

F N Bruun, G J McGarrity, W S Blakemore, L L Coriell
PMCID: PMC274281  PMID: 818110

Abstract

The epidemiology of hospital infections due to Pseudomonas aeruginosa was investigated by pyocin typing. The typing method, which determined the pyocin activity of clinical isolates of P. aeruginosa on 27 indicator strains, was 43.7% reproducible, but elimination of 9 indicator strains doubled the reproducibility and yielded more readable pyocin inhibition zones. Seventy-eight of 1,084 isolates (7.2%) were untypable. In the second part of the study, P. aeruginosa was isolated from 110 patients (5.4% of all admissions) in a 3-month period and typed with the revised method. Twenty pyocin types were identified, 10 of which were obtained from five or more patients. P. aeruginosa was isolated from 45 of 353 environmental samples, including water fountains, ice machines, bar soaps, and germicide solutions for toilet brushes. Twenty percent of the environmental samples were untypable but, among typable strains, the five most common environmental strains were the same as the strains most frequently isolated from patients. The organism was frequently isolated from noses (39%), throats (39%), and stools (29%) of patients with P. aeruginosa infections or colonizations in urine, sputum, surgical wounds, or skin lesions. Six of eight patients had P. aeruginosa in their tracheostomy wounds. Autoinfections by strains already acquired on carrier sites may be significant.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Al-Dujaili A. H., Harris D. M. Pseudomonas aeruginosa infection in hospital: a comparison between 'infective' and 'environmental' strains. J Hyg (Lond) 1975 Oct;75(2):195–201. doi: 10.1017/s0022172400047227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BANNAN E. A., JUDGE L. F. BACTERIOLOGICAL STUDIES RELATING TO HANDWASHING. 1. THE INABILITY OF SOAP BARS TO TRANSMIT BACTERIA. Am J Public Health Nations Health. 1965 Jun;55:915–922. doi: 10.2105/ajph.55.6.915. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bergan T. Epidemiological markers for Pseudomonas aeruginosa. 1. Serogrouping, pyocine typing--and their interrelations. Acta Pathol Microbiol Scand B Microbiol Immunol. 1973 Feb;81(1):70–80. [PubMed] [Google Scholar]
  4. Bobo R. A., Newton E. J., Jones L. F., Farmer L. H., Farmer J. J., 3rd Nursery outbreak of Pseudomonas aeruginosa: epidemiological conclusions from five different typing methods. Appl Microbiol. 1973 Mar;25(3):414–420. doi: 10.1128/am.25.3.414-420.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bruun J. N., Digranes A. Survival of gram-negative bacilli and Candida albicans in hexachlorophene preparations and other disinfectants. Scand J Infect Dis. 1971;3(3):235–238. doi: 10.3109/inf.1971.3.issue-3.10. [DOI] [PubMed] [Google Scholar]
  6. Buck A. C., Cooke E. M. The fate of ingested Pseudomonas aeruginosa in normal persons. J Med Microbiol. 1969 Nov 4;2(4):521–525. doi: 10.1099/00222615-2-4-521. [DOI] [PubMed] [Google Scholar]
  7. DARRELL J. H., WAHBA A. H. PYOCINE-TYPING OF HOSPITAL STRAINS OF PSEUDOMONAS PYOCYANEA. J Clin Pathol. 1964 May;17:236–242. doi: 10.1136/jcp.17.3.236. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Edmonds P., Suskind R. R., Macmillan B. G., Holder I. A. Epidemiology of pseudomonas aeruginosa in a burn hospital: evaluation of serological, bacteriophage, and pyocin typing methods. Appl Microbiol. 1972 Aug;24(2):213–218. doi: 10.1128/am.24.2.213-218.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Falcão D. P., Mendonça C. P., Scrassolo A., De Almeida B. B., Hart L., Farmer L. H., Farmer J. J., 3rd Nursery outbreak of severe diarrhoea due to multiple strains of Pseudomonas aeruginosa. Lancet. 1972 Jul 1;2(7766):38–40. doi: 10.1016/s0140-6736(72)91291-3. [DOI] [PubMed] [Google Scholar]
  10. Farmer J. J., 3rd, Herman L. G. Epidemiological fingerprinting of Pseudomonas aeruginosa by the production of and sensitivity of pyocin and bacteriophage. Appl Microbiol. 1969 Nov;18(5):760–765. doi: 10.1128/am.18.5.760-765.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Farmer J. J., 3rd Mnemonic for reporting bacteriocin and bacteriophage types. Lancet. 1970 Jul 11;1(7663):96–96. doi: 10.1016/s0140-6736(70)92663-2. [DOI] [PubMed] [Google Scholar]
  12. Gillies R. R., Govan J. R. Typing of Pseudomonas pyocyanea by pyocine production. J Pathol Bacteriol. 1966 Apr;91(2):339–345. doi: 10.1002/path.1700910207. [DOI] [PubMed] [Google Scholar]
  13. Jacob F., Blobel H., Scharmann W. Die Typisierung von Pseudomonas aeruginosa mit titrierten Pyocinen. Zentralbl Bakteriol Orig A. 1973 Oct;224(4):472–477. [PubMed] [Google Scholar]
  14. Jones L. F., Zakanycz J. P., Thomas E. T., Farmer J. J., 3rd Pyocin typing of Pseudomonas aeruginosa: a simplified method. Appl Microbiol. 1974 Feb;27(2):400–406. doi: 10.1128/am.27.2.400-406.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lowbury E. J., Thom B. T., Lilly H. A., Babb J. R., Whittall K. Sources of infection with Pseudomonas aeruginosa in patients with tracheostomy. J Med Microbiol. 1970 Feb;3(1):39–56. doi: 10.1099/00222615-3-1-39. [DOI] [PubMed] [Google Scholar]
  16. McGarrity G. J., Coriell L. L. Bacterial contamination of children's soap bubbles. Am J Dis Child. 1973 Feb;125(2):224–226. doi: 10.1001/archpedi.1973.04160020056010. [DOI] [PubMed] [Google Scholar]
  17. Merrikin D. J., Terry C. S. Variability of pyocine type and pyocine sensitivity in some strains of Pseudomonas aeruginosa. J Appl Bacteriol. 1972 Dec;35(4):667–672. doi: 10.1111/j.1365-2672.1972.tb03748.x. [DOI] [PubMed] [Google Scholar]
  18. Mulholland S. G., Bruun J. N. A study of hospital urinary tract infections. J Urol. 1973 Aug;110(2):245–248. doi: 10.1016/s0022-5347(17)60177-0. [DOI] [PubMed] [Google Scholar]
  19. Mulholland S. G., McGarrity G. J., Ross O. A., Greenhalgh P. J., Blakemore W. S. Experience with detailed surveillance of nosocomial infection. Surg Gynecol Obstet. 1975 Jun;140(6):941–945. [PubMed] [Google Scholar]
  20. PYRAH L. N., GOLDIE W., PARSONS F. M., RAPER F. P. Control of Pseudomonas pyocyanea infection in a urological ward. Lancet. 1955 Aug 13;269(6885):314–317. doi: 10.1016/s0140-6736(55)92306-0. [DOI] [PubMed] [Google Scholar]
  21. Wysocki J. P., Mulholland G., McGarrity G. J., Ximenes M., Blakemore W. S. The role of wounds in the epidemiology of nosocomial infections due to Pseudomonas aeruginosa. Invest Urol. 1974 Mar;11(5):370–373. [PubMed] [Google Scholar]
  22. Zabransky R. J., Day F. E. Pyocine typing of clinical strains of Pseudomonas aeruginosa. Appl Microbiol. 1969 Feb;17(2):293–296. doi: 10.1128/am.17.2.293-296.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

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