Abstract
The effect of cetylpyridinium chloride (CPC) on pathogenic fungi and Nocardia asteroides was studied. Sputa seeded with each of 11 organisms (Aspergillus flavus; Aspergillus fumigatus, Blastomyces dermatitidis, Candida albicans, Coccidioides immitis, Cryptococcus neoformans, Geotrichum candidum, Histoplasma capsulatum; Nocardia asteroides, Paracoccidioides brasiliensis, and Sporothrix schenckii) were treated with CPC and kept for 2, 5 and 9 days. The CPC reagent used (0.5% CPC and 0.5% sodium chloride) is one the Mycobacteriolgoy Branch at the Center for Disease Control added to sputa before shipping them to laboratories for recovery of mycobacteria. None of the organisms tested survived this treatment, and none was recovered on mycological or mycobacteriological media. Seeded sputa containing these organisms were also tested with a second CPC reagent (0.02% CPC and 0.5% sodium chloride) and held for 2, 5 and 9 days. A few colonies of A. flavus, A. fumigatus, and N. asteroides were recovered from these samples. Neither the morphology of the fungi nor their stainability by the fluorescent antibody method was affected by treatment with the reagent containing 0.5% CPC. However, the background material in smears from the 0.5% CPC-treated samples retained the conjugate, and this made weakly fluorescing organisms more difficult to detect. The 0.5% CPC treatment did not alter the morphology of N. asteroides or its ability to be stained with Kinyoun acid-fast stain.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Grover S., Junnarkar R. V. Pulmonary mycoses. A study of five interesting cases. Indian J Med Sci. 1966 Oct;20(10):689–697. [PubMed] [Google Scholar]
- HUSSEY L. M. FINAL ARTICLE OF PULMONARY FUNGEOUS INFECTIONS. West Med Med J West. 1965 Jun;6:181–185. [PubMed] [Google Scholar]
- KAPLAN W., CLIFFORD M. K. PRODUCTION OF FLUORESCENT ANTIBODY REAGENTS SPECIFIC FOR THE TISSUE FORM OF COCCIDIOIDES IMMITIS. Am Rev Respir Dis. 1964 May;89:651–658. doi: 10.1164/arrd.1964.89.5.651. [DOI] [PubMed] [Google Scholar]
- KAPLAN W., IVENS M. S. Fluorescent antibody staining of Sporotrichum schenckii in cultures and clinical materials. J Invest Dermatol. 1960 Sep;35:151–159. doi: 10.1038/jid.1960.99. [DOI] [PubMed] [Google Scholar]
- KAPLAN W., KAUFMAN L. SPECIFIC FLUORESCENT ANTIGLOBULINS FOR THE DETECTION AND IDENTIFICATION OF BLASTOMYCES DERMATITIDIS YEAST-PHASE CELLS. Mycopathol Mycol Appl. 1963 Jun 15;19:173–180. doi: 10.1007/BF02051245. [DOI] [PubMed] [Google Scholar]
- KAUFMAN L., KAPLAN W. Preparation of a fluorescent antibody specific for the yeast phase of Histoplasma capsulatum. J Bacteriol. 1961 Nov;82:729–735. doi: 10.1128/jb.82.5.729-735.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kaplan W. Direct fluorescent antibody tests for the diagnosis of mycotic diseases. Ann Clin Lab Sci. 1973 Jan-Feb;3(1):25–29. [PubMed] [Google Scholar]
- Kaufman L., Blumer S. Occurrence of serotypes among Histoplasma capsulatum strains. J Bacteriol. 1966 Apr;91(4):1434–1439. doi: 10.1128/jb.91.4.1434-1439.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Neimeister R. P., Patterson N. T., Cocklin J. H., Harada G. F. The isolation of pathogenic fungi from sodium hydroxide-processed sputum specimens from patients suspected to have tuberculosis. Am J Clin Pathol. 1971 Aug;56(2):201–203. doi: 10.1093/ajcp/56.2.201. [DOI] [PubMed] [Google Scholar]
- Pidcoe V., Kaufman L. Fluorescent-antibody reagent for the identification of Cryptococcus neoformans. Appl Microbiol. 1968 Feb;16(2):271–275. doi: 10.1128/am.16.2.271-275.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Richards O. W., Waters P. A new interference exciter filter for fluorescence microscopy of fluorescein-tagged substances. Stain Technol. 1967 Nov;42(6):320–321. [PubMed] [Google Scholar]
- Smithwick R. W., Stratigos C. B., David H. L. Use of cetylpyridinium chloride and sodium chloride for the decontamination of sputum specimens that are transported to the laboratory for the isolation of Mycobacterium tuberculosis. J Clin Microbiol. 1975 May;1(5):411–413. doi: 10.1128/jcm.1.5.411-413.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]