Abstract
Streptococci from clinical isolates were studied for their ability to produce pigment in stab cultures in Columbia agar. Serological grouping of these organisms was done by counterimmunoelectrophoresis using Burroughs-Wellcome antisera. In this group of isolates, 66 of the 68 organisms grouped as B by serological testing produced pigment in the Columbia agar stab cultures. Pigment was not produced by any of the other 36 streptococci studied (11 group A, 9 group C, 4 group D, and 12 nongroupable). The use of the Columbia agar stab culture is recommended as a rapid and simple test for recognition of group B streptococci. The counterimmunoelectrophoresis test is also suggested as a convenient, rapid, and sensitive method for grouping the streptococci.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Dajani A. S. Rapid identification of beta hemolytic streptococci by counterimmunoelectrophoresis. J Immunol. 1973 Jun;110(6):1702–1705. [PubMed] [Google Scholar]
- Darling C. L. Standardization and evaluation of the CAMP reaction for the prompt, presumptive identification of Streptococcus agalactiae (Lancefield group B) in clinical material. J Clin Microbiol. 1975 Feb;1(2):171–174. doi: 10.1128/jcm.1.2.171-174.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Edwards E. A., Larson G. L. Serological grouping of hemolytic streptococci by counter-immunoelectrophoresis. Appl Microbiol. 1973 Dec;26(6):899–903. doi: 10.1128/am.26.6.899-903.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Facklam R. R., Padula J. F., Thacker L. G., Wortham E. C., Sconyers B. J. Presumptive identification of group A, B, and D streptococci. Appl Microbiol. 1974 Jan;27(1):107–113. doi: 10.1128/am.27.1.107-113.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fallon R. J. The rapid recognition of Lancefield group B haemolytic streptococci. J Clin Pathol. 1974 Nov;27(11):902–905. doi: 10.1136/jcp.27.11.902. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hill H. R., Riter M. E., Menge S. K., Johnson D. R., Matsen J. M. Rapid identification of group B streptococci by counterimmunoelectrophoresis. J Clin Microbiol. 1975 Feb;1(2):188–191. doi: 10.1128/jcm.1.2.188-191.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hwang M. N., Ederer G. M. Rapid hippurate hydrolysis method for presumptive identification of group B streptococci. J Clin Microbiol. 1975 Jan;1(1):114–115. doi: 10.1128/jcm.1.1.114-115.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pollock H. M., Dahlgren B. J. Distribution of streptococcal groups in clinical specimens with evaluation of bacitracin screening. Appl Microbiol. 1974 Jan;27(1):141–143. doi: 10.1128/am.27.1.141-143.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]