Skip to main content
. 2009 Sep;15(9):1716–1728. doi: 10.1261/rna.1724409

FIGURE 7.

FIGURE 7.

The snoRNA (snR35) requirement can be satisfied with a different snoRNA containing the corresponding Ψ guide elements. (A) Structure of the hybrid snoRNA created by replacing the Ψ guide elements in snR36 snoRNA with those from snR35. The substituted nucleotides in the hybrid snoRNA are shown in bold and the predicted interactions between the mutant snoRNA and pre-rRNA are given. (B) The hybrid RNA can fully complement loss of the snR35 snoRNA. Growth properties were compared on solid medium for the (−)3b test strain transfromed with expression plasmids for hybrid and parental snoRNAs. (C) The impaired polysome pattern was restored to normal by the mutant snoRNA. Polysome patterns are shown for two mutant strains (−1a and −3b) expressing or not the hybrid snoRNA. (D) A two-nucleotide deletion in snR35 inactivates its modification function. The nucleotides deleted are shown in bold. (E) The modification-defective mutant snoRNA (snR35m) does not restore cell growth. Growth properties were compared for (−)3b test cells expressing a plasmid-borne deletion mutant snR35 (snR35m), or control guide snoRNAs (snR56 and snR70). (F) The impaired polysome pattern in (−)3b cells could not be restored to normal by expressing the inactive snR35 (snR35m) or natural snR36. Control, Ys602 cells with an empty vector.