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. 1976 Apr;3(4):443–448. doi: 10.1128/jcm.3.4.443-448.1976

Evaluation of the Bacti-Lab streptococci culture systems for selective recovery and identification of group A streptococci.

P W Freeburg, J M Buckingham
PMCID: PMC274321  PMID: 816808

Abstract

Twenty-nine lots of Bacti-Lab streptococci culture plates containing nalidixic acid, neomycin, and amphotericin B were inoculated with 25 strains of group A, B, C, F, and G strepotocci, 13 strains of nonstrepotcoccal organisms representative of the throat flora, and 198 fresh clinical thorat specimens to determine the accuracy of the plates in identifying group A streptococci. With the Bacti-Lab test plates 98.87% of the beta-hemolytic streptococcal strains tested produced growth 95.51% yielding more than 50 colonies. Of the nonstreptococcal beta-hemolytic and non-beta-hemolytic organism tested, 79.54% were inhibited by the selective media. When bacitracin disks were used for presumptive identification group A streptococci from clinical thorat specimens, 12.00% false positive and 0.00% false negative results were obtained as measured by the direct flourescent-antibody and Lancefield grouping methods.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blanchette L. P., Lawrence C. Group A streptococcus screening with neomycin blood agar. Am J Clin Pathol. 1967 Oct;48(4):441–443. doi: 10.1093/ajcp/48.4_ts.441. [DOI] [PubMed] [Google Scholar]
  2. Chitwood L. A., Jennings M. B., Riley H. D., Jr Time, cost, and efficacy study of identifying group A streptococci with commercially available reagents. Appl Microbiol. 1969 Aug;18(2):193–197. doi: 10.1128/am.18.2.193-197.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ederer G. M., Herrmann M. M., Bruce R., Matsen J. M., Chapman S. S. Rapid extraction method with pronase B for grouping beta-hemolytic streptococci. Appl Microbiol. 1972 Feb;23(2):285–288. doi: 10.1128/am.23.2.285-288.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Facklam R. R., Padula J. F., Thacker L. G., Wortham E. C., Sconyers B. J. Presumptive identification of group A, B, and D streptococci. Appl Microbiol. 1974 Jan;27(1):107–113. doi: 10.1128/am.27.1.107-113.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Freeburg P. W. Rapid fluorescent-antibody stain technique with group A streptococci. Appl Microbiol. 1970 Jun;19(6):940–942. doi: 10.1128/am.19.6.940-942.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HOLLINGER N. F., LINDBERG L. H. Delayed recovery of streptococci from throat swabs. Am J Public Health Nations Health. 1958 Sep;48(9):1162–1169. doi: 10.2105/ajph.48.9.1162. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. MAXTED W. R. The use of bacitracin for identifying group A haemolytic streptococci. J Clin Pathol. 1953 Aug;6(3):224–226. doi: 10.1136/jcp.6.3.224. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. MOODY M. D., PADULA J., LIZANA D., HALL C. T. EPIDEMIOLOGIC CHARACTERIZATION OF GROUP A STREPTOCOCCI BY T-AGGLUTINATION AND M-PRECIPITATION TESTS IN THE PUBLIC HEALTH LABORATORY. Health Lab Sci. 1965 Jul;2:149–162. [PubMed] [Google Scholar]
  9. STREAMER C. W., WILLIAMS P. M., WANG W. L., JOHNSON R. S., McGUIRE C. D., ABELOW I. J., GLASER R. J. Identification of group A streptococci. Bacitracin disc and fluorescent antibody techniques compared with the Lancefield precipitin method. Am J Dis Child. 1962 Aug;104:157–160. [PubMed] [Google Scholar]
  10. Vincent W. F., Gibbons W. E., Gaafar H. A. Selective medium for the isolation of streptococci from clinical specimens. Appl Microbiol. 1971 Nov;22(5):942–943. doi: 10.1128/am.22.5.942-943.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

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