Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1976 May;3(5):480–485. doi: 10.1128/jcm.3.5.480-485.1976

Radioimmunoassay for measuring antibodies specific for group B streptococcal types Ia, Ib, Ic, II, and III.

H W Wilkinson, W L Jones
PMCID: PMC274335  PMID: 58870

Abstract

The object of this study was to develop a test that would measure antibodies directed against group B streptococcal types Ia, Ib, Ic, II, and III. The type-specific carbohydrate antigens were purified, labeled with 125I, and used to develop a radioimmunoassay. This procedure should be particularly useful in testing human sera for group B type-specific antibodies, since it requires very small quantities of antigens and measures primary type antigen-antibody interactions.

Full text

PDF
480

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baker C. J., Barrett F. F., Gordon R. C., Yow M. D. Suppurative meningitis due to streptococci of Lancefield group B: a study of 33 infants. J Pediatr. 1973 Apr;82(4):724–729. doi: 10.1016/s0022-3476(73)80606-7. [DOI] [PubMed] [Google Scholar]
  2. Baker C. J., Barrett F. F. Group B streptococcal infections in infants. The importance of the various serotypes. JAMA. 1974 Nov 25;230(8):1158–1160. [PubMed] [Google Scholar]
  3. Baker C. J., Barrett F. F. Transmission of group B streptococci among parturient women and their neonates. J Pediatr. 1973 Dec;83(6):919–925. doi: 10.1016/s0022-3476(73)80524-4. [DOI] [PubMed] [Google Scholar]
  4. CURTIS S. N., KRAUSE R. M. ANTIGENIC RELATIONSHIPS BETWEEN GROUPS B AND G STREPTOCOCCI. J Exp Med. 1964 Oct 1;120:629–637. doi: 10.1084/jem.120.4.629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Eickhoff T. C., Klein J. O., Mortimer E. A., Jr, Wehrle P. F. Letter: The issue of prophylaxis of neonatal group B streptococcal infections. J Pediatr. 1973 Dec;83(6):1097–1100. doi: 10.1016/s0022-3476(73)80569-4. [DOI] [PubMed] [Google Scholar]
  6. FARR R. S. A quantitative immunochemical measure of the primary interaction between I BSA and antibody. J Infect Dis. 1958 Nov-Dec;103(3):239–262. doi: 10.1093/infdis/103.3.239. [DOI] [PubMed] [Google Scholar]
  7. Franciosi R. A., Knostman J. D., Zimmerman R. A. Group B streptococcal neonatal and infant infections. J Pediatr. 1973 Apr;82(4):707–718. doi: 10.1016/s0022-3476(73)80604-3. [DOI] [PubMed] [Google Scholar]
  8. Gotschlich E. C. A simplification of the radioactive antigen-binding test by a double label technique. J Immunol. 1971 Sep;107(3):910–911. [PubMed] [Google Scholar]
  9. Gotschlich E. C., Rey M., Triau R., Sparks K. J. Quantitative determination of the human immune response to immunization with meningococcal vaccines. J Clin Invest. 1972 Jan;51(1):89–96. doi: 10.1172/JCI106801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hey D. J., Hall R. T., Burry V. F., Thurn A. N. Neonatal infections caused by group B streptococci. Am J Obstet Gynecol. 1973 May 1;116(1):43–47. doi: 10.1016/0002-9378(73)90881-8. [DOI] [PubMed] [Google Scholar]
  11. Lancefield R. C., McCarty M., Everly W. N. Multiple mouse-protective antibodies directed against group B streptococci. Special reference to antibodies effective against protein antigens. J Exp Med. 1975 Jul 1;142(1):165–179. doi: 10.1084/jem.142.1.165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lerner P. I. Meningitis caused by Streptococcus in adults. J Infect Dis. 1975 May;131 (Suppl):S9–16. doi: 10.1093/infdis/131.supplement.s9. [DOI] [PubMed] [Google Scholar]
  13. Quirante J., Ceballos R., Cassady G. Group B beta-hemolytic streptococcal infection in the newborn. I. Early onset infection. Am J Dis Child. 1974 Nov;128(5):659–665. doi: 10.1001/archpedi.1974.02110300069009. [DOI] [PubMed] [Google Scholar]
  14. Steere A. C., Aber R. C., Warford L. R., Murphy K. E., Feeley J. C., Hayes P. S., Wilkinson H. W., Facklam R. R. Possible nosocomial transmission of group B streptococci in a newborn nursery. J Pediatr. 1975 Nov;87(5):784–787. doi: 10.1016/s0022-3476(75)80311-8. [DOI] [PubMed] [Google Scholar]
  15. Wilkinson H. W., Eagon R. G. Type-specific antigens of group B type Ic streptococci. Infect Immun. 1971 Nov;4(5):596–604. doi: 10.1128/iai.4.5.596-604.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wilkinson H. W., Facklam R. R., Wortham E. C. Distribution by serological type of group B streptococci isolated from a variety of clinical material over a five-year period (with special reference to neonatal sepsis and meningitis). Infect Immun. 1973 Aug;8(2):228–235. doi: 10.1128/iai.8.2.228-235.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wilkinson H. W. Immunochemistry of purified polysaccharide type antigens of group B streptococcal types Ia, Ib, and Ic. Infect Immun. 1975 Apr;11(4):845–852. doi: 10.1128/iai.11.4.845-852.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES